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Tissue culture method for improving genetic transformation efficiency of petunia hybrida

A technology of genetic transformation efficiency and genetic transformation, applied in the field of tissue culture to improve the genetic transformation efficiency of petunia, can solve the problems of uneven genetic transformation efficiency, different leaf size and thickness, etc.

Pending Publication Date: 2021-09-03
河南省农业科学院园艺研究所
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Problems solved by technology

However, in the existing petunia genetic transformation operations, the genetic transformation materials used are mainly leaves, and the limitation of material types makes the actual transformation efficiency and transformation effect significantly related to the growth status of leaves; on the other hand, due to the selection of leaves The lack of a unified standard, the difference in leaf size and thickness, etc., resulting in uneven subsequent genetic transformation efficiency

Method used

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  • Tissue culture method for improving genetic transformation efficiency of petunia hybrida
  • Tissue culture method for improving genetic transformation efficiency of petunia hybrida
  • Tissue culture method for improving genetic transformation efficiency of petunia hybrida

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Embodiment

[0059] Based on a large number of actual experiments, limited by the genotype reasons of some petunias (the specific reasons need to be further studied), some petunia species / variety (such as R27, M1, R149, P. inflata species or varieties) are not suitable for direct use in genetic transformation. In order to explore how to solve this technical problem, the inventors took genotypes M1 and R27 as examples, and obtained the F1 generation (M1×R27, M1 is the female parent, R27 is the male parent) through conventional hybridization, and then further selected the genotypes according to the traits. Based on the individual offspring of the research, and through asexual reproduction methods such as tissue culture or cuttings, genetic transformation materials with stable and uniform characteristics can be obtained, which can lay a certain foundation for improving the efficiency of genetic transformation. During the technical research process, the standardized tissue culture method prov...

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Abstract

The application belongs to the technical field of horticulture, and particularly relates to a standardized tissue culture method for improving genetic transformation efficiency of petunia hybrida. The tissue culture method is suitable for tissue culture application of petunia during transgenic genetic transformation, and specifically comprises the steps of explant selection, dip dyeing liquid preparation, leaf dip dyeing and co-culture, screening culture, rooting culture, acclimatization and transplantation and the like. In the application, by taking specific plasmid construction as an example, key factors of related tissue culture technologies influencing genetic transformation efficiency in a petunia genetic transformation process are discussed and optimized, so that a certain technical foundation can be laid for standardized operation and standardized tissue culture system construction in the related genetic transformation process; and a certain technical foundation can be laid for petunia hybridoma related genetic transformation system construction and new petunia hybridoma variety breeding, so that the method has relatively good scientific research value and practical significance.

Description

technical field [0001] The application belongs to the technical field of horticulture, and in particular relates to a standardized tissue culture method for improving the genetic transformation efficiency of petunia. Background technique [0002] Petunias ( Petunia hybrida (J. D. Hooker) Vilmorin ) is a widely used flower bed and flower mirror plant, and it is also an ideal model plant for studying ornamental traits (flower color, flower fragrance, flower shape, etc.), and has the following advantages: (1) The life cycle is short, about 3 months. Complete the whole process from seed germination to flowering, and can grow about 4 generations in a year; (2) Asexual reproduction such as cuttings and tissue culture is simple, and the reproduction efficiency is high; (3) Petunia P.inflata and P. axillaris The genome sequencing work has been completed, which provides very favorable conditions for carrying out comparative genome research and is a good material for studying ...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/29A01H4/00A01H5/00A01H6/00
CPCC12N15/8205C12N15/8218C07K14/415A01H4/002A01H4/008
Inventor 张和臣符真珠王慧娟高杰李艳敏高凯王二强王利民袁欣郑谊李涵蒋卉冯乃曦张严凡成濮生
Owner 河南省农业科学院园艺研究所
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