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Application of PhCLV3 and PhCLV1 genes in regulating ornamental value of petunia plants

A petunia, ornamental technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problem of application prospect to be explored, and achieve the effect of good application prospect, high flowering density and improving ornamental value.

Active Publication Date: 2022-08-02
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At this stage, the function of CLV-WUS in the growth and development of petunia has not been reported yet.
In addition, with the continuous deepening of research on petunia flower color and petal double petals, individual petunia plant type-related genes EVERGREEN have been identified (Castel et al., 2010; Rebocho et al., 2008), but Its application prospect remains to be explored

Method used

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  • Application of PhCLV3 and PhCLV1 genes in regulating ornamental value of petunia plants
  • Application of PhCLV3 and PhCLV1 genes in regulating ornamental value of petunia plants
  • Application of PhCLV3 and PhCLV1 genes in regulating ornamental value of petunia plants

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Experimental program
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Effect test

Embodiment 1

[0029] Example 1: Preparation of petunia targeting PhCLV3 and PhCLV1 gene targets

[0030] According to the gene sequences of PhCLV3 and PhCLV1, 20bp was selected as the target in the coding regions of the two genes, and the two genes were knocked out in a targeted manner, using the CRISPR-P v2.0 online network tool (http: / / cbi.hzau.edu.cn / CRISPR2 / ) to select sgRNA targets. First, use the p_U6p vector as a template to amplify the p_U6p-Target-gRNA expression cassette, and use target primers such as PhCLV3-g1-F containing the target site and Bsal1 restriction site and the public primer U-R to amplify the gRNA fragment (primer sequences are as follows ). The amplified sgRNA_U6 fragment was ligated into p51, p61 and other series of intermediate vectors through the first Golden Gate reaction system, and the positive clones were identified by p-F / R primers; the intermediate vector plasmid with the correct band was passed through the second The secondary Golden Gate edge ligation...

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Abstract

The invention discloses an application of PhCLV3 and PhCLV1 genes in regulating and controlling the ornamental value of petunia plants. The invention finds that the PhCLV3 and PhCLV1 genes are knocked out by using a gene editing technology, a single mutant with lost functions shows that the number of petals is increased, the phenotype of double mutants with lost functions of the two genes is more violent, scarce head-shaped flower buds are presented, the petals are double petals, the petal area is increased, the flowering density is increased or the plant height is reduced, and the number of the petals is increased. The ornamental value of petunia as a flower plant is greatly improved, and it is indicated that the PhCLV3 and PhCLV1 genes have important regulation and control effects in the growth and development process of petunia. Therefore, the method has a good application prospect in the aspect of character improvement of petunia hybrida flowers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to the application of PhCLV3 and PhCLV1 genes in regulating the ornamental properties of petunia plants. Background technique [0002] The petunia is native to South America and is the most common flower plant in the nightshade family. Under suitable conditions, petunia can bloom all year round, and it has become one of the most popular horticultural flowers in the world because of the diversity of color and flower organ morphology. In the United States, the cultivation and consumption of petunia ranks first among herbaceous flower plants, and many varieties have won flower bed plant awards. Petunia is one of the most popular flowers in Japan. The introduction of petunia varieties in my country is relatively late, mainly in the 1980s. Today's commercialized petunia was cultivated by scientists through conventional breeding in the past two centuries. A series of inbred germp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/02A01H6/82
CPCC07K14/415C12N15/827C12N15/8261C12N15/8216Y02A40/146
Inventor 许操唐伶俐邹玉盼
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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