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Method for culturing monopetalous petunia hybrida test-tube flowers

The technology of a test-tube flower and a cultivation method, which is applied in the field of plant tissue culture, can solve the problems that petunia test-tube flowers have not been reported, can not obtain test-tube flowers, and are difficult to grow roots, etc., to achieve rich new varieties, rich colors, and production The effect of simple method

Inactive Publication Date: 2015-01-07
甘肃省农业科学院生物技术研究所
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  • Application Information

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Problems solved by technology

This method aims to overcome the problems of self-fertilization of petunias and the preservation of the excellent characters of cuttings that are difficult to root, and finally potted plants. This method cannot obtain test-tube flowers.
[0004] In summary, the preparation method of Petunia test-tube flowers has not been reported in the prior art at present

Method used

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  • Method for culturing monopetalous petunia hybrida test-tube flowers
  • Method for culturing monopetalous petunia hybrida test-tube flowers
  • Method for culturing monopetalous petunia hybrida test-tube flowers

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Embodiment 1

[0018] A method for cultivating single petunia test-tube flowers, the specific operation process is as follows:

[0019] 1. Purchase petunia seeds of the "dream" pink veined variety in the market;

[0020] 2. Sterilization and germination of external body:

[0021] Put 40-50 petunia seeds in a sterile triangular flask with sterile gloves, pour 30ml of 75% alcohol, shake and sterilize for 1min, pour out the alcohol waste liquid, add 50ml of sterile water to rinse, and pour out the waste water , add 50ml of 0.1% mercuric chloride solution and sterilize by shaking for 5-7min, pour out the mercuric chloride waste liquid, add 100ml of sterile water to shake and wash, repeat four times, pour out the final waste liquid, and use tweezers to remove the sterilized Seeds are placed on the surface of MS medium to germinate, the culture temperature is 22-24°C, the light intensity is 2000lx, and the photoperiod is 10hrs / d (the seeds are very small and require fine manipulation, when pourin...

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Abstract

The invention discloses a method for culturing monopetalous petunia hybrida test-tube flowers. The method comprises the following steps: sterilizing seeds of a long-day monopetalous petunia hybrida variety serving as an explant; putting the seeds on the surface of an MS medium and germinating; cutting stems with 2-3 leaves after sprouts grow out, inserting the stems into a subculture medium for continuous subculture; cutting stem tips with 4-6 leaves from subculture seedlings, and inoculating to a flower bud medium; and generating flower buds and blooming by cultural environmental induction to finally form test-tube flowers. The method has the beneficial effects that: the plant size, flower bud induction and flower bud blooming of petunia hybrida in test tubes are manually controlled by virtue of a tissue culture technology so as to finally obtain test-tube flowers; the test-tube flowers have the characteristics that flowers are of various colors, the flowering phase can last for one month, and blooming is continuous; and the method is simple and reliable, has production practicality, and can increase new varieties of test-tube flowers.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for cultivating single petunia test-tube flowers. Background technique [0002] The use of flowers and plants to beautify the environment is the pursuit of human beings for a comfortable life. With the continuous improvement of people's demand for appreciation of flowers, test tube flowers have appeared on the market. They are famous for their small plants and cultivated in delicate glass bottles. It is Plants are miniaturized and cultured in sterile glass bottles using tissue culture technology, and the beautifully shaped test tube glass bottles provide growth space for plants in the bottles. The inoculated plants grow and bloom in a small space. The test-tube flowers have the advantages of no need for fertilization and management, and a long flowering period for viewing. They are popular with urban people and are also called "miniature flowers". In 2004, the "Finge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 厚毅清赵瑛罗俊杰王方郭嘉玮欧巧明张运晖裴怀弟陈子萱李淑洁苏子桐
Owner 甘肃省农业科学院生物技术研究所
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