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Centella asiatica polyploid and inducing breeding method thereof

A polyploid, Centella asiatica technology, applied in the field of Centella asiatica polyploid and its induced breeding, can solve the problems of high toxicity of colchicine, high price of colchicine, and high operation requirements of experimenters, and achieves high performance. Stability and reproducibility, improving the success rate of induction, the effect of high success rate of induction

Active Publication Date: 2016-07-27
GUILIN SANJIN PHARMACEUTICALS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, colchicine-induced polyploids have insurmountable shortcomings: colchicine is highly toxic and pollutes the environment; colchicine is expensive and expensive to use in experiments, so it is necessary to seek low-pollution, low-cost, low-toxicity induction Induced chromosome doubling
This application includes a method for inducing and cultivating Centella asiatica species in the laboratory, which needs to be completed in the cultivation room, and has the disadvantages of high requirements for experimenters, cumbersome steps, and high labor costs
Moreover, the content of the active substance in the prepared polyploid variety was not detected in the patent application, and the experimental results were inaccurate

Method used

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  • Centella asiatica polyploid and inducing breeding method thereof
  • Centella asiatica polyploid and inducing breeding method thereof
  • Centella asiatica polyploid and inducing breeding method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for breeding Centella asiatica polyploidy, comprising the following steps:

[0037] 1. Transplant wild Centella asiatica into the culture medium; the temperature for transplanting wild Centella asiatica is 12-15°C; the humidity is 80%, the medium is the cultivation substrate of medicinal dregs, and compound fertilizer is added as the base fertilizer. The medicinal dregs cultivation medium added with compound fertilizer contains available N1000~1200mg / kg, available P300~350mg / kg, available K6000~6500mg / kg, organic matter 300~350g / kg, exchangeable Ca25~35cmol / kg, exchangeable Mg1 .5~3cmol / kg, effective Zn40~50mg / kg, effective Fe300~320mg / kg, effective B0.1~0.4mg / kg; pH is 6.0~6.5.

[0038] 2. prepare inducer solution; described inducer solution is that concentration is 30mg / L sulfamethasulfone, and is added with 2wt% dimethyl sulfoxide; 3. absorbent cotton is wrapped in centella asiatica overgrown leaf growth point, with inducer soak the absorbent cotton with th...

Embodiment 2

[0041] A method for breeding Centella asiatica polyploidy, comprising the following steps:

[0042] 1. Transplant wild Centella asiatica into the culture medium; the temperature for transplanting wild Centella asiatica is 12-16° C.; the humidity is 70-75%. The culture medium is the dregs cultivation substrate, and compound fertilizer is added as the base fertilizer. The medicinal dregs cultivation medium added with compound fertilizer contains available N1000~1200mg / kg, available P300~350mg / kg, available K6000~6500mg / kg, organic matter 300~350g / kg, exchangeable Ca25~35cmol / kg, exchangeable Mg1 .5~3cmol / kg, effective Zn40~50mg / kg, effective Fe300~320mg / kg, effective B0.1~0.4mg / kg; pH is 6.0~6.5.

[0043] 2. Prepare an inducer solution; the inducer solution has a concentration of 40mg / L amisulfame, and is added with 2wt% dimethyl sulfoxide. 3. Wrap the absorbent cotton on the growing point of Centella asiatica clumped leaves, soak the absorbent cotton with an inducer solution;...

Embodiment 3

[0046] A method for breeding Centella asiatica polyploidy, comprising the following steps:

[0047] 1. Transplant wild Centella asiatica into the culture medium; the temperature for transplanting wild Centella asiatica is 12-18°C; the humidity is 75%, the medium is the cultivation substrate of medicinal dregs, and compound fertilizer is preferably added as the base fertilizer. The medicinal dregs cultivation substrate added with compound fertilizer contains 1000-1200mg / kg of available N, 300-350mg / kg of P, 6000-6500mg / kg of K, 300-350g / kg of organic matter, 25-35cmol / kg of exchangeable Ca, Exchangeable Mg1.5~3cmol / kg, effective Zn40~50mg / kg, effective Fe300~320mg / kg, effective B0.1~0.4mg / kg; pH is 6.0~6.5.

[0048] 2. Prepare an inducer solution; the inducer solution has a concentration of 50 mg / L amisulfame, and is added with 3wt% dimethyl sulfoxide. 3. Wrap the absorbent cotton on the growing point of Centella asiatica bushy leaves, soak the absorbent cotton with inducer so...

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Abstract

The invention relates to the field of plant breeding and concretely relates to a centella asiatica polyploid and an inducing breeding method thereof. The inducing breeding method comprises transplanting wild centella asiatica to a medium, preparing an inducer solution containing 30-50mg / L of orizalina and 1-3wt% of dimethyl sulfoxide, wrapping centella asiatica fasciated leaf growth points through degreased cotton, soaking the degreased cotton with the inducer solution, carrying out treatment under natural illumination conditions for 2-4 days, carrying out detection through a chromosome microscope to determine a tetraploid sample and carrying out asexual expanding propagation and preservation. The method realizes centella asiatica in-vivo induction, has a high induction conversion rate and greatly improves medicinal effective substance content of the centella asiatica polyploidy.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a centella asiatica polyploid and a method for inducing breeding thereof. Background technique [0002] Centella asiatica is a plant of the genus Centella asiatica of the Umbelliferae family. There are about 20 species in the world. Only one species of Centella asiatica is produced in my country, which is an important Chinese herbal medicine. Thousands of years of history. Widely distributed in various counties and cities in Guangxi, it is one of the Zhuang medicines with Guangxi national characteristics. [0003] The medicinal materials of Centella asiatica mainly come from the mining of wild resources, and the quality controllability is poor. Digging will inevitably result in the destruction of Centella asiatica habitat and wild populations. Therefore, the implementation of GAP (Good Agricultural Practice) of Chinese medicinal materials is of great significance to ensure the qua...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 邹节明蒋水元钟小清
Owner GUILIN SANJIN PHARMACEUTICALS CO LTD
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