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Method for cultivating triploid dendrobium huoshanense by crossbreeding method of tetraploid plants and diploid plants

A technology for cross-breeding of Dendrobium huoshanense, applied in the directions of botanical equipment and methods, application, and plant genetic improvement, etc., can solve the problem that triploid Dendrobium huoshanica has not been publicly reported.

Active Publication Date: 2015-03-11
WEST ANHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, only the cultivation methods of triploid Dendrobium officinale and Dendrobium spring have been published and authorized. So far, there has been no public report on the triploid Dendrobium Huoshanense (chromosomal karyotype index is 2n=3x=57) and its cultivation methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0027] (1) The plants of variety A and variety B are planted and cultivated according to conventional methods;

[0028] (2) Carry out aseptic sowing to the original plant seeds of different varieties in solid medium, obtain the diploid protocorm of variety A and variety B plants, the solid medium is 1 / 2MS medium with macroelements halved, add 0.2 g / L 2, 4-D and 0.2g / L NAA, adjust the sucrose concentration to 5%, add 10% coconut milk, culture temperature in the range of temperature difference between day and night at 25-18 ℃, light time 10-12 h, light Intensity 2000-3000 Lux, color temperature 4500-5500 K;

[0029] (3) Prepare diploid protoplasts of variety A and variety B plants for cell fusion, the preparation method of protoplasts is enzymatic hydrolysis, and the cell fusion method adopts electric shock method;

[0030] (4) Continue culturing the fused cells to the callus, take the callus cell staining and pressing, microscopically check the chromosomal ploidy, and detect t...

Embodiment 2

[0036] (1) The plants of variety A and variety B are planted and cultivated according to conventional methods;

[0037] (2) Carry out aseptic sowing to the original plant seeds of different varieties in solid medium, obtain the diploid protocorm of variety A and variety B plants, the solid medium is 1 / 2MS medium with macroelements halved, add 0.2 g / L 2,4-D and 0.2g / L NAA, adjust the sucrose concentration to 6%, add 15% potato juice, cultivate the temperature in the range of temperature difference between day and night at 25-18 ℃, light time 10-12 h, light Intensity 2000-3000 Lux, color temperature 4500-5500 K;

[0038] (3) Prepare diploid protoplasts of variety A and variety B plants, and carry out cell fusion. The preparation method of protoplast is enzymatic preparation, and the cell fusion method adopts electric shock method;

[0039] (4) Continue culturing the fused cells to the callus, take the callus cell staining and pressing, microscopically check the chromosomal ploi...

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Abstract

The invention discloses a method for cultivating triploid dendrobium huoshanense by a crossbreeding method of tetraploid plants and diploid plants. The method comprises the following steps: (1) dendrobium huoshanense species which are from different producing areas and have characteristics of good stress resistance, rapid growth, thick stalk, long stem, many tillers, low fiber content and high active ingredient content are preferably selected as polyploidy cultivated original plant parents; (2) seeds of the different species of original plants undergo aseptic sowing in a solid medium so as to obtain diploid protocorms; (3) protoplasts of the different species of diploid protocorms are prepared and cell fusion is carried out; (4) detected tetraploid plants undergo asexual tissue culture so as to obtain tetraploid plant seedlings; (5) the tetraploid plant seedlings undergo hardening-seedling acclimatization and cultivation to flowering; (6) the tetraploid plants and diploid plants undergo artificial pollination and crossbreeding, and fructification is completed to obtain triploid dendrobium huoshanense seeds; and (7) the triploid seeds undergo aseptic sowing and culture so as to obtain a lot of triploid seedlings.

Description

technical field [0001] The invention relates to a biological breeding method for medicinal plants of the genus Dendrobium orchidaceae, in particular to a method for cultivating triploid Dendrobium Huoshanense by using the hybrid breeding method of tetraploid plants and diploid plants. Background technique [0002] Dendrobium huoshanense (scientific name: Dendrobium huoshanense C.Z.Tang et S.J.Cheng) is known internationally as the "giant panda in the medicine world". years of history. Native to my country's Anhui Dabie Mountains and other places, it is the first protected variety among more than 200 endangered and scarce varieties in the national major modern Chinese medicine project. It is the best product among medicinal Dendrobium, and it is also a rare and precious Chinese herbal medicine unique to Huoshan County with geographical indications of origin. Both "Shen Nong's Materia Medica" and "Compendium of Materia Medica" have records. The polysaccharides and alkaloids...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 邓辉陈存武陈乃富张莉戴军
Owner WEST ANHUI UNIV
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