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Method for inducing new strain of tetraploid dendrobium with seeds protocorm

A protocorm-like and tetraploid technology is applied in the field of seed protocorm-like cultivation of new tetraploid Dendrobium dendrobii orchid, which can solve the problem that no polyploid Dendrobium dendrobii orchid has been reported yet, and improve the frequency of polyploid mutagenesis. , the effect of low input and high output

Inactive Publication Date: 2008-02-20
NANKAI UNIV
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Problems solved by technology

However, so far, the orchids of Dendrobium at home and abroad are all diploid species, and there is no report of polyploid Dendrobium orchids.

Method used

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  • Method for inducing new strain of tetraploid dendrobium with seeds protocorm

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Embodiment

[0027] At the beginning of October 2003, the autumn Dendrobium orchid was purchased from Yanzhou Sanyi Company in Shandong Province, and the same plant was artificially pollinated on the 10th and 16th of that year. On June 22, 2004 (246 days), cut off the capsules, and in the ultra-clean workbench, first sterilize with 75% alcohol for 1 minute, then sterilize with 0.1% mercury liter for 10 minutes, rinse 5 times with sterile distilled water, In the direction of the longitudinal axis, the capsules were cut open with a sterile scalpel, and the seeds of Dendrobium nodii were inoculated in 1 / 2 MS liquid medium, and cultured with intermittent shaking at 20-25°C. After cultivating for 60 days, transfer to 1 / 2MS liquid medium containing 0.02% Oryzalin (amisululine), mutagenesis treatment for 150 to 180 hours, and then transfer to 1 / 2MS solid medium, at a photoperiod of 14 hours / Day, light intensity 3000Lux, continue to cultivate under the condition of room temperature 20~25 ℃, until...

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Abstract

The invention relates to a method for cultivating the novel variety of tetraploid dendrobium by use of the seed protocorm (PLBs). After the artificial pollination, the immature dendrobium seed in the development stage is carried out non-symbiotic sproution cultivation for 60 days in the 1 / 2MS liquid culture medium, which is then transferred into the 1 / 2MS liquid culture medium containing 0.02 percent Oryzalin for process and then transferred into the 1 / 2MS solid culture medium for continuous cultivation; after that, till the dendrobium seedling is shaped, the dendrobium is not planted, transplanted and managed by a conventional method until the dendrobium blooms. The invention overcomes the defect of generally low mutagenesis frequency of the polyploid and has better appreciation; moreover, the method is easy and practical, which increases the mutagenesis frequency (by over 60 percent) of the polyploidy in a large scale and provides enough polyploidy (over hundreds of plants) obtained one time to be used for the seed selection with high application value. Besides, the invention is applicable to the large-scale production with the advantages of small investment and high output.

Description

technical field [0001] The invention relates to a method for cultivating new varieties of orchids, in particular to a method for cultivating new tetraploid dendrobium orchids by using protobulbs (PLBs) of seeds. Background technique [0002] Autumn Dendrobium orchid (Dendrobium sp.), also known as Butterfly Dendrobium, is mostly a hybrid variety bred from the parent of the tropical native species Dendrobium Phalaenopsis native to New Guinea. Autumn Dendrobium is a new type of high-grade cut orchid variety, which has high appreciation value and a large market demand, and occupies an important position in the international and domestic flower industry. In recent years, many species of Dendrobium orchids have been introduced into mainland my country. But, so far, the orchids of Dendrobium dendrobii both at home and abroad are diploid species, and there is no report on the orchids of Dendrobium dendrobium polyploid. The most direct effect after polyploidy is the enlargement of...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01H1/02A01H4/00C12Q1/68A01G1/00
Inventor 李秀兰陈力陈成彬宋文芹陈瑞阳
Owner NANKAI UNIV
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