Fluorescence in situ hybridization (FISH) method for fish chromosomes

A fluorescence in situ hybridization, chromosome technology, applied in fluorescence/phosphorescence, biochemical equipment and methods, microbial determination/inspection, etc. clear image effect
CN101974622AInactive Publication Date: 2011-02-16DALIAN OCEAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN OCEAN UNIV
Publication Date
2011-02-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a fluorescence in situ hybridization (FISH) method for fish chromosomes. In the method, on the basis that a metaphase split phase specimen with a clear image and good chromosome spreading is obtained, a probe is marked by FISH technology and a nick translation method by taking Biotin-16-dUTP as a marker, a hybridization signal is amplified at two stages and a human 5.8S+28SrDNA probe is clearly positioned on a polyploid fish chromosome nucleolus organizer region (NOR). The chromosome ploidy and a karyotype of a polyploid are compared and analyzed by observing the chromosomal localization of ribosome 5.8S+28SrDNA on a polyploid fish, namely, the polyploid fish is proved to be a genetic polyploidy or an evolution polyploidy and an autopolyploid or an allopolyploid byanalyzing.
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Description

Technical field:

[0001] The invention relates to a fluorescent in situ hybridization method for fish chromosomes, in particular to a method with high sensitivity, strong specificity and accurate positioning, which is beneficial for analyzing whether polyploid fish are genetic polyploids or evolutionary polyploids, and whether they are polyploid fish Fluorescence in situ hybridization method for autopolyploid or allopolyploid fish chromosomes. Background technique:

[0002] In aquatic animals, natural polyploidy is common. Among the karyotypes of freshwater fishes reported in China, polyploid types were found in many kinds of fish. Polyploid fish have incomparable advantages over diploid fish because of their growth advantage, population yield and disease resistance. Therefore, many polyploid species have become important economic fish or important breeding objects . At present, studies on the number and karyotype of fish chromosomes have been reported in corresponding lit...

Claims

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