Tissue culture method for inducing populus yunnanensis dode tetraploid in combination with colchicine

A technology of colchicine and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of poor repeatability, difficult operation, large workload, etc., and achieve stable polyploidy characteristics, poor repeatability, The effect of heavy workload

Inactive Publication Date: 2013-02-13
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mashkina et al. (Mashkina O S, Burdaeva L M, Belozerova M M, et al. Method of obtaining diploid pollen of woody species[J]. Lesovedenie, 1989a, 1:19-25.) used autumn in the first mitosis of Populus alba zygotes. Treat it with narcissine to obtain tetraploid plants, but it needs to process a large amount of materials every day, with heavy workload, poor repeatability, many chimeras and low induction rate
Zhang Zhiyi et al. (Zhang Zhiyi, Li Fenglan. Study on Poplar Chromosome Doubling Technology and Triploid Breeding (Ⅰ)-Pollen Chromosome Doubling Technology [J]. Journal of Beijing Forestry University, 1992, 14(3):52-58.), Kang Xiangyang (Kang Xiangyang, Zhang Pingdong, Gao Peng, et al. Discovery of a new way of colchicine-induced poplar triploid[J]. Journal of Beijing Forestry University, 2004,26(1):1-4.), Wang Jun (Wang Jun .Research on Polyploid Induction Technology of Qingyang School Species[D].Beijing: Ph.D. Dissertation of Beijing Forestry University. 2009.) Using poplar flower buds or tidbits as materials, applying colchicine to induce gamete or zygote chromosome doubling to produce 2n gametes , the polyploid material is obtained after fertilization, and there is no chimera material, and the induction effect is good, but the method is difficult and technically demanding, and it is not easy to operate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, collect the biennial branches of healthy and healthy trees without disease and insect pests in the Populus yunnanensis forest. After all the leaves on the branches are removed, they are placed in room temperature for about 30 days in hydroponics, and after new leaves grow, the petioles are cut off with scissors, which are used as materials for polyploid induction of Populus yunnanensis.

[0015] Step 1. After all the leaves on the branches are removed, they are placed in room temperature for 30 days of hydroponics, and after new leaves grow, the petioles are cut off with scissors, as materials for polyploid induction of Populus yunnanensis;

[0016] Step 2: Put the collected poplar petiole into a beaker, add 2% sodium hypochlorite, shake for 6-8 minutes, rinse with sterile water for 3 times, add 70% alcohol for disinfection for 40 seconds, and then add 0.1% HgCl 2 Disinfect for 7 minutes, and finally rinse 5 times with sterile water;

[0017] Step 3. Prep...

Embodiment 2

[0022] Embodiment 2. Gather the biennial branches of the populus yunnanensis, which grow robustly and have no damage by diseases and insect pests. After all the leaves on the branches are removed, they are placed in room temperature for about 30 days in hydroponics, and after new leaves grow, the petioles are cut off with scissors, which are used as materials for polyploid induction of Populus yunnanensis.

[0023] Step 1. After all the leaves on the branches are removed, they are placed in room temperature for 30 days of hydroponics, and after new leaves grow, the petioles are cut off with scissors, as materials for polyploid induction of Populus yunnanensis;

[0024] Step 2: Put the collected poplar petiole into a beaker, add 2% sodium hypochlorite, shake for 6-8 minutes, rinse with sterile water for 3 times, add 70% alcohol for disinfection for 40 seconds, and then add 0.1% HgCl 2 Disinfect for 7 minutes, and finally rinse 5 times with sterile water;

[0025] Step 3. Prep...

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PUM

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Abstract

The invention relates to the technology of plant tissue culture, and in particular relates to a tissue culture method for inducing populus yunnanensis dode tetraploid in combination with colchicine. The method taking petiole of populus yunnanensis dode as a tissue culture material in the process of tissue culture is characterized by comprising the following steps of: adding colchicine into a populus yunnanensis dode petiole differential medium to induce populus yunnanensis dode to generate polyploidy, wherein the treatment time is 20-30 days; carrying out differentiation culture again in a populus yunnanensis dode petiole differential medium in which colchicine is not added after the treatment; cutting the part which is obvious in variation on the differentiated cluster buds; repeatedly cultivating on the differential medium for 5-6 times, so that the populus yunnanensis dode petiole differential medium is stable in polyploidization variation; and performing rooting on the clump seedlings which are stably varied, so that the tissue culture seedlings of the tetraploid populus yunnanensis dode can be obtained. The method provided by the invention is the tissue culture method for inducing the populus yunnanensis dode tetraploid in combination with the colchicine, which is easy and simple to operate and can effectively reduce the chimera.

Description

technical field [0001] The invention relates to plant tissue culture technology, especially the tissue culture method of Populus yunnanensis. Background technique [0002] Yunnan poplar ( Populus yunnanensis Dode) is a member of the genus Populus (Salicaceae) in the family Salicaceae ( Populus ) Tacamahaca (section Tacamahaca) tree species, tree, more than 20 meters high, produced in Yunnan, Guizhou and Sichuan, born in mountains at an altitude of 1300-3200m. It has the characteristics of fast growth, strong adaptability, early maturity, and easy reproduction. This tree species is also a unique native tree species in Southwest my country, and it is a rare and extremely valuable poplar germplasm resource distributed in low-latitude and high-altitude areas in the country and even in the world. However, Populus yunnanensis has the disadvantages of being susceptible to poplar black spot, poplar canker, susceptible to stem borer and has many branches, and its excellent chara...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 辛培尧何承忠李根前姜华田斌孙正海唐军荣王大玮辛静
Owner SOUTHWEST FORESTRY UNIVERSITY
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