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Bacteria strain of antagonistic plant pathogenic bacteria and application of bacteria strain

A technology of plant pathogenic bacteria and strains, applied in the field of microorganisms, can solve problems such as prevention and control of drug residues and environmental pollution, and achieve the effect of efficient prevention and control

Active Publication Date: 2016-11-23
BEIJING FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented new type of microorganism called Pymobactrerium permeabilis (PB) produces an effective ingredient that prevents harmful organisms like insects and other pests from infecting crops worldwide. These beneficial properties make this agent useful for controlling various types of plants such as cotton flies, tomatoes, citrus flowers, rice roots, wheat strawberries, cereals, vegetables, etc., without causing damage by these harmful agents.

Problems solved by technology

This patented technical problem addressed in this patent relates to controlling vegetal diseases like downy mildew (LMS), root rot, stem blight, scabies, leather rust, black stalk rottage, crop loss, forest fires, drought, poorly managed agriculture, and threat poses challenges due to its ability to infect multiple generations without being able to develop resistance against existing treatments. Biologically controlled substances offer promise because their effectiveness depends upon specific conditions within each generation.

Method used

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  • Bacteria strain of antagonistic plant pathogenic bacteria and application of bacteria strain
  • Bacteria strain of antagonistic plant pathogenic bacteria and application of bacteria strain
  • Bacteria strain of antagonistic plant pathogenic bacteria and application of bacteria strain

Examples

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Embodiment 1

[0062] Embodiment 1 Isolation, screening and identification of Paenibacillus profundus (Paenibacillus profundus) XZQ-20 of the present invention

[0063] 1. Isolation and screening of Paenibacillus profundus XZQ-20

[0064] The bacterial strain of the present invention is collected from poplar rhizosphere soil on the campus of Beijing Forestry University in Haidian District, Beijing.

[0065] 1A) Take 5g of the soil at the place where the thickness of poplar rhizosphere soil layer is 5-25cm, place it in a triangular flask filled with 45mL of sterile water, shake it at 28°C at 180rpm for 30min on a shaker, let it stand, and the obtained bacterial suspension liquid (ie 10 -1 The soil dilution solution, that is, the soil solution diluted 10 times); Then use 10 times of gradient dilution method to dilute successively to obtain 10 -3 、10 -4 、10 -5 Concentration gradient diluent; then take 0.1mL from each concentration gradient suspension and apply it on the LB medium plate with...

Embodiment 2

[0086] Embodiment 2 Exploration of the biological characteristics of Paenibacillus profundus (Paenibacillus profundus) XZQ-20 of the present invention

[0087]1A) Pick a loop (inoculation loop) from the Paenibacillus profundus XZQ-20 strain activated on the slant, and inoculate it into 100mL LB liquid medium (each 1000mL contains tryptone 10g, yeast extract 5g, NaCl 10g, pH 7.0-7.2) in a 250mL Erlenmeyer flask, then cultured at 30°C and 200rpm with shaking for 1d to prepare the seed liquid of Paenibacillus profundus XZQ-20 strain, wherein the seed liquid of Paenibacillus profundus XZQ-20 strain OD 600 The value is about 1.564.

[0088] 1B) Inoculate the Paenibacillus profundus XZQ-20 strain seed liquid into the 100mL Erlenmeyer flask containing 40mL of different salt (NaCl) concentrations and pH values ​​of the NB culture solution according to the inoculation amount of 1% (V / V), at 30°C After 2 days of shaking culture at 200rpm, 0.4mL of Paenibacillus profundus XZQ-20 strain...

Embodiment 3

[0093] Embodiment 3 Determination of protease-producing ability of Paenibacillus profundus (Paenibacillus profundus) XZQ-20 bacterial strain of the present invention

[0094] 1A) Pick a circle of activated Paenibacillus profundus XZQ-20, and plant it in the center of a plate filled with skimmed milk medium (each 1000mL contains 50g skimmed milk powder, 15g agar, pH 7.2-7.4) , and then place the plate in a constant temperature incubator at 30°C for upside-down culture, and observe whether the skimmed milk around the colony and on the plate is decomposed and becomes transparent after 1, 3, 5, and 7 days respectively. The appearance of a transparent circle means that the strain can produce protease, and the absence of a transparent circle means that the strain cannot produce protease.

[0095] The assay results show that the antagonistic bacteria Paenibacillus profundus XZQ-20 strain can form an obvious transparent circle ( Figure 4 ), showing that Paenibacillus profundus XZQ-2...

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Abstract

The invention discloses a bacteria strain of antagonistic plant pathogenic bacteria and application of the bacteria strain. The antagonistic bacteria are Paenibacillus profundus XZQ-20, and the microbial preservation number of the Paenibacillus profundus XZQ-20 is CGMCC No. 12556. The Paenibacillus profundus XZQ-20 is separated from rhizosphere soil of poplar, can produce protease and inhibit the growth of plant pathogenic bacteria such as Lonsdalea quercina, Pseudomonas syringae, Erwinia carotovora and Xanthomonas juglandis, and can be used for preventing and treating plant diseases caused by the plant pathogenic bacteria above. The antagonistic bacteria with biological preventing and treating potential are high in preventing and treating efficiency, wide in preventing and treating range, good in environment safety and promising in development and application prospect.

Description

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Claims

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Application Information

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Owner BEIJING FORESTRY UNIVERSITY
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