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Tissue-culturing rapid propagation method of Actinidia macrosperma

A kiwifruit and tissue culture technology is applied in the field of tissue culture and rapid propagation of large-seeded kiwifruit, which can solve problems such as the tissue culture research report of large-seeded kiwifruit, which can eliminate the invasion of diseases and insect pests and pesticide residues, and the equipment is simple. Small footprint effect

Inactive Publication Date: 2006-09-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the kiwifruit species that have been successfully tissue-cultured include Chinese kiwifruit (A. kolomikta) and multi-flowered kiwifruit (A.latifolia), etc. (Zhang Yuanji, 1996; Margarida OM, 1991; Kovae J, 1993; Jia Shuzhong, 1991), no research report on tissue culture of large-seeded kiwifruit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Selection of explants: Take the branches of large-seeded kiwifruit from Hangzhou and midsummer, wash them with tap water, rinse with running water for half an hour, cut into 1-2cm stem segments with buds, put them in a triangular flask, 75% alcohol Treated for 30sec, rinsed with sterile water, disinfected with 0.1% mercuric chloride for 12min, rinsed with sterile water for 4-5 times, and inoculated into the induction medium under aseptic conditions, with a contamination rate of 38%. Taking the branches from Hangzhou and winter, and disinfecting them according to the same disinfection method, the pollution rate is as high as 87%.

Embodiment 2

[0040] Take the branches of big-seeded kiwifruit from Fuyang Mountain in Hangzhou in midsummer, wash them with tap water, rinse with running water for half an hour, cut them into 1-2cm long sections with buds and stems, treat with 75% alcohol for 30sec, and then rinse with sterile water After sterilizing with 0.1% mercuric chloride for 12min, rinsed with sterile water for 4-5 times, inoculated in induction medium MS+BA 5μmol / L+NAA 1μmol / L under sterile conditions. The culture temperature is 24-26℃, the daily light time is 16h, and the light intensity is 35μmolm -2 s -1 , cultured for 10-15d. The germinated shoots obtained on the induction medium were cut off and transferred to the growth medium MS+BA 2μmol / L+GA 3μmol / L+ZT 4μmol / L, the culture temperature was 24-26℃, the daily light time was 16h, and the light intensity was 35μmolm -2 s -1 , cultured for 15-20d. The multiplication of buds reached 11.6 times, the average height of plants reached 9.28cm, but the leaves were y...

Embodiment example 3

[0042] Take the branches and leaves of the big-seeded kiwifruit from Hangzhou in midsummer, wash them with tap water, rinse with running water for half an hour, cut them into segments with buds, put them in a triangular flask, treat with 75% alcohol for 30 seconds, and then use no After rinsing with bacteria water, disinfect with 0.1% mercuric chloride for 12 minutes, rinse with sterile water for 4-5 times, and inoculate them in induction medium MS+BA 5μmol / L+NAA 1μmol / L under sterile conditions. The culture temperature is 24-26℃, the daily light time is 16h, and the light intensity is 35μmolm -2 s -1 , cultured for 10-15 days. The germinated shoots obtained on the induction medium were cut off and transferred to the proliferation medium MS+BA 6μmol / L+NAA 1μmol / L, the culture temperature was 24-26°C, the daily light time was 16h, and the light intensity was 35μmolm -2 s -1 , cultured for 15-20 days. The bud multiplication was 4.27, and the average seedling height was 3.85c...

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PUM

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Abstract

The invention provides a method of rapid breeding of kiwi fruit with large seed by tissue culture. The invention comprises steps of selecting and treating explant, inducing culture medium, root culturing and replanting, etc. The invention takes MS culture medium as basis and adds BA, NAA, GA, ZT and other external plant growth regulator to induce the growth of axillary bud of the said kiwi fruit and strengthen sprout and root. The root growth rate is as high as 100%, the average bud height is 6.28 cm and the average replanting survival rate is 91% after 35 days culture. The invention solves the technique problem of rapid breeding of said kiwi fruit and provides a method of short period, high breeding rate and low cost for the industrial production of kiwi fruit with large seed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a tissue culture rapid propagation method for large-seeded kiwifruit. Background technique [0002] Plants are a natural treasure house of medicines. People have a long history of using medicinal plants. About 75% of the world's population uses plants as a source of medicine for treating and preventing diseases (Xing Jianmin, 2001). Humans have discovered many drugs with high physiological activity from plants, and the drugs derived from plants account for more than 25% of the total amount of drugs (Zheng Guangzhi, 1987). Our country's traditional Chinese herbal medicine has a history of thousands of years, and it is still widely used in our country and many countries and regions. However, because the traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a large amount of wild plant resources, when the amount of collection and consumption...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G17/00A01G7/00C12N5/04
Inventor 姜维梅赵云鹏王晓韵李树坤傅承新
Owner ZHEJIANG UNIV
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