A kind of method for rapid propagation of sealwort
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A Polygonatum chinensis, rapid technology, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of unsatisfactory proliferation coefficient and rooting coefficient, few research reports on proliferation culture and rooting culture, etc. The effect of rooting and strengthening seedlings, expanding materials, and saving tissue culture costs
Inactive Publication Date: 2021-02-19
SHAOXING UNIVERSITY
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Rhizomes, leaves, anthers, rhizome seeds, and seeds of Polygonatum polyflora can be used as explants, but there are few research reports on proliferation culture and rooting culture, and the proliferation coefficient and rooting coefficient are not ideal.
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[0021] A method for rapid propagation of Polygonatum, comprising the following steps:
[0022] 1. Obtaining and disinfection of explants: get Rhizoma Polygonatum seeds for disinfection; in the step ①, get Rhizoma Rhizoma Rhizoma seeds and soak and rinse them under flowing tap water for 1-2 hours and then carry out disinfection treatment. Isolation, its interior is in a sterile state, and it is very easy to disinfect the surface.
[0023] Disinfect the surface with 75% alcohol for 5 minutes on the ultra-clean workbench, then disinfect with 15% hydrogen peroxide for 30 minutes, and rinse with sterile water 3 times.
[0024] The reason why seeds are superior to other explants is that from the perspective of material collection and explant disinfection, stems, petioles or tubers are relatively close to the soil, and are more seriously polluted by miscellaneous bacteria. Once the disinfection is not clean, it is easy to cause medium pollution. The explants die, but the seeds are d...
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Abstract
The invention discloses a method for the rapid propagation of Rhizoma Polygonatum, which comprises the following steps: ① obtaining and disinfecting explants; ② cultivating seeds; ③ synchronously cultivating bud propagation and rooting; ④ inducing callus culturing; ⑤ inducing callus Cultivation of seedlings; ⑥ cultivation of strong seedlings. The present invention aims at inducing and culturing the seedlings grown from Polygonatum seeds as explants, selects the best hormone ratio to make them develop into complete plants, provides a new technology for the tissue culture of Polygonatum, and rapidly propagates virus-free Polygonatum seedlings , to establish a production system for highly efficient industrialized Polygonatum tissue culture seedlings, to provide a large number of high-quality seedlings for my country's Polygonatum industry, and to make up for the shortage of Polygonatum seedlings market.
Description
technical field [0001] The invention relates to the technical field of modern agricultural seedling cultivation, in particular to a method for rapid propagation of Polygonatum. Background technique [0002] Polygonatum cyrtonema Hua (Polygonatum cyrtonema Hua) is a perennial herb of the genus Polygonatum in the Liliaceae family. Polygonatum multiflora is used as medicine with rhizomes. In addition to active substances such as Polygonatum polysaccharides, steroidal saponins, and flavonoids, it also contains alkaloids, lignans, anthraquinones, and various essential amino acids and trace elements. Immune stimulation, immune promotion, anti-radiation, anti-tumor, anti-aging, hypoglycemic, hypolipidemic, anti-fatigue, hypoxia resistance, etc., used clinically to treat diabetes, coronary heart disease, hyperlipidemia, tuberculosis, lymphatic tuberculosis , leukopenia, diarrhea, constipation, insomnia and other diseases, has high health care and medicinal value. In recent years, ...
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