A method for multiplying homozygous mice with ifn-γ gene deficiency and its application

Abstract

The invention discloses a method for multiplying IFN-γ gene-deficient homozygous mice and its application. The method for multiplying is to firstly mate purchased IFN-γ gene-deficient heterozygotes, and then breed the pure mice in the progeny. A large number of IFN-γ gene-deficient homozygous mice can be obtained by mating the male and female homozygotes in the progeny, realizing the expansion of IFN-γ gene-deficient homozygous mice, and obtaining IFN-γ gene-deficient homozygous mice have normal constitution than the directly purchased IFN-γ gene-deficient homozygous mice, which can be better used for the establishment of Cryptosporidium infection model in vitro. Very low doses can cause rapid and chronic infection, and The mouse mortality rate in the process is low, which can meet the requirement of a long research period for Cryptosporidium infection. Moreover, the FN-γ gene-deficient homozygous mice obtained after the multiplication can be directly purely mated to produce offspring, achieving a large number of multiplication without complicated pure cross mating.

Description

technical field [0001] The invention relates to the technical field of animal experiment model breeding, and more specifically, to a method for multiplying homozygous mice with IFN-γ gene deficiency and its application. Background technique [0002] Cryptosporidium is a single-celled parasite that can infect a variety of animals, including humans, and is a member of the apicomplexan phylum. Gastrointestinal disease caused by Cryptosporidium, known as cryptosporidiosis, is one of the common causes of diarrhea in children worldwide and can have long-term adverse effects on children's growth and development, as well as those with AIDS and the immunocompromised life-threatening. In 1907, the famous parasitologist Tyzzer found a large number of worms when he sliced ​​the mucous membrane of mice, and named it C. muris. In 1912, Tyzzer found similar worms in the small intestine of mice, but thought it was another worm species. , because this species is smaller than C. muris and t...

AI Technical Summary

Problems solved by technology

However, in the prior art, the purchased IFN-γ gene-deficient homozygous mice are generally used for infection experiments. Compared with normal mice, the directly purchased IFN-γ gene-deficient homozygous mice develop slower and have weaker constitutions. , cannot reproduce effectively, and may trigger a stress response when inoculating Cryptosporidium oocysts, resulting in the death of mice, or death due to weak constitution during the Cryptosporidium infection experiment, which cannot meet the long-term requirements for Cryptosporidium infection. Study Cycle Requirements

On the other hand, due to the need for a large number of IFN-γ gene-deficient homozygous mice for experiments, and the high price of homozygotes leads to high experimental costs

At present, there is no report on the successful expansion of IFN-γ gene-deficient homozygous mice

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