A kind of method for propagating and propagating salt knot wood

A technology for assimilating juniper trees and assimilating branches is applied in the biological field and can solve the problems of small seeds, difficult management of seedlings for sowing and reproduction, and asynchrony of germination.

Inactive Publication Date: 2018-04-17
山西师范大学
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AI Technical Summary

Problems solved by technology

Due to the small size of the seeds, Saltwood has the disadvantages of long germination time, asynchronous germination, and difficult management of sowing and propagating seedlings.

Method used

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  • A kind of method for propagating and propagating salt knot wood
  • A kind of method for propagating and propagating salt knot wood
  • A kind of method for propagating and propagating salt knot wood

Examples

Experimental program
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Embodiment 1

[0048] Example 1. Expansion of salt knot wood

[0049] 1. Seed germination of salt knot wood

[0050] Inoculate the seeds of S. sylvestris after conventional disinfection (70% ethanol disinfection for 1 minute and 0.1% mercury for 8-10 minutes) in a germination medium to germinate and cultivate for 4-5 months, and germinate and cultivate every month The obtained seedlings are transferred to a fresh germination medium to obtain seedlings.

[0051] The formula of the above germination medium is as follows: Mix NaCl, sucrose, agar and MS liquid medium to obtain a germination medium, wherein the concentrations of NaCl, sucrose, and agar are 200mmol·L respectively -1 , 20g·L -1 , 8g·L -1 , The pH is 5.8.

[0052] The conditions for the above germination culture are the temperature of 25℃ and the light intensity of 120μmol·m -2 ·S -1 ,Light time 12h·d -1 .

[0053] 2. Assimilation branch axillary bud induction culture

[0054] A 0.5 cm long assimilation branch was cut from the seedlings obtai...

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Abstract

The invention discloses a propagation method for halocnemum strobilaceum. The propagation method for the halocnemum strobilaceum includes the following steps that 1, a halocnemum strobilaceum explant is subjected to induction culture in an induction medium, and an axillary bud clustered seedling obtained after induction culture is obtained; 2, an assimilation branch of the axillary bud clustered seedling obtained after induction culture is cut out and subjected to subculture multiplication culture in the induction medium, and an axillary bud clustered seedling obtained after subculture multiplication culture is obtained; 3, an assimilation branch of the axillary bud clustered seedling obtained after subculture multiplication culture is cut out and subjected to rooting culture in a rooting medium, and a seedling obtained after rooting culture is obtained; 4, the seedling obtained after rooting culture is sequentially hardened and transplanted, and propagation of the halocnemum strobilaceum is achieved. It is proved through experiments that the method lays a foundation for research on high-quality germplasm preservation and rapid propagation and further genetic transformation and molecular biology.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a method for multiplying salt knot wood. Background technique [0002] Salicaria is a kind of halophyte in the genus Salicaria of Chenopodiaceae, distributed on saline-alkali soils from northern Africa to Mediterranean Europe to western Asia (Qu et al. 2008). my country is mainly distributed in Xinjiang Junggar Basin, Tarim Basin and Hexi Corridor. It is a dominant perennial semi-shrub species that grows in saline deserts. It has extremely wide adaptability to the saline environment (Gao Ruiru et al. 2007, 2009). The seeds of knot wood contain a certain amount of oil, which is of edible value; the plant also contains alkali, which is the raw material for making alkali; in addition, the crude protein content is also high, which can be used as autumn feed for animals in desert areas (Kong Lingshao et al. , 1995). Its stems and leaves become fleshy and shortened into branches. It is also cal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 刘维仲陈惠遆卫国
Owner 山西师范大学
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