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Propagation method for halocnemum strobilaceum

A technology of salt-knotted wood and assimilated branches, which is applied in the biological field and can solve the problems of difficult management of seedlings, long germination time, small seeds, etc.

Inactive Publication Date: 2016-05-11
山西师范大学
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the small size of the seeds, Saltwood has the disadvantages of long germination time, asynchronous germination, and difficult management of sowing and propagating seedlings.

Method used

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  • Propagation method for halocnemum strobilaceum
  • Propagation method for halocnemum strobilaceum
  • Propagation method for halocnemum strobilaceum

Examples

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Embodiment 1

[0048] Embodiment 1, the propagation of salt knot wood

[0049] 1. Seed germination of Saltwood

[0050] Inoculate the seeds of S. chinensis after routine disinfection (70% ethanol disinfection for 1 minute with 0.1% mercuric chloride for 8-10 minutes) in the germination medium and germinate and cultivate them for 4-5 months, and germinate and cultivate them every month. The resulting seedlings were transferred to fresh germination medium to obtain seedlings.

[0051] The formula of the above-mentioned germination medium is as follows: mix NaCl, sucrose, agar and MS liquid medium to obtain a germination medium, wherein the concentrations of NaCl, sucrose and agar are respectively 200mmol L -1 , 20g·L -1 , 8g·L -1 , pH value is 5.8.

[0052] The conditions for the above germination culture were temperature at 25°C and light intensity at 120 μmol·m -2 ·s -1 , light time 12h·d -1 .

[0053] 2. Induction culture of assimilated branch axillary buds

[0054] The 0.5 cm long a...

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Abstract

The invention discloses a propagation method for halocnemum strobilaceum. The propagation method for the halocnemum strobilaceum includes the following steps that 1, a halocnemum strobilaceum explant is subjected to induction culture in an induction medium, and an axillary bud clustered seedling obtained after induction culture is obtained; 2, an assimilation branch of the axillary bud clustered seedling obtained after induction culture is cut out and subjected to subculture multiplication culture in the induction medium, and an axillary bud clustered seedling obtained after subculture multiplication culture is obtained; 3, an assimilation branch of the axillary bud clustered seedling obtained after subculture multiplication culture is cut out and subjected to rooting culture in a rooting medium, and a seedling obtained after rooting culture is obtained; 4, the seedling obtained after rooting culture is sequentially hardened and transplanted, and propagation of the halocnemum strobilaceum is achieved. It is proved through experiments that the method lays a foundation for research on high-quality germplasm preservation and rapid propagation and further genetic transformation and molecular biology.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for propagating and propagating chrysophyllum. Background technique [0002] Saltwood is a halophyte belonging to the Chenopodiaceae genus Saltwood, distributed in saline-alkaline lands from northern Africa to Mediterranean Europe to western Asia (Qu et al. 2008). my country is mainly distributed in the Junggar Basin, Tarim Basin, and Hexi Corridor in Xinjiang. It is a perennial semi-shrub dominant plant species growing in saline deserts, and has extremely wide adaptability to saline environments (Gao Ruiru et al. 2007, 2009). There is a certain amount of oil in the seeds of Knuckles, which has edible value; the plant also contains alkali, which is a raw material for making alkali; in addition, the crude protein content is also high, which can be used as autumn feed for animals in desert areas (Kong Lingshao et al. ,1995). Its stems and leaves are succulent and shortened int...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 刘维仲陈惠遆卫国
Owner 山西师范大学
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