Propagation method for homozygous mice with IFN-gamma gene defect and application of propagation method

Abstract

The invention discloses a propagation method for homozygous mice with an IFN-gamma gene defect and an application of the propagation method. The propagation method comprises the steps of firstly, carrying out mating on purchased heterozygotes with an IFN-gamma gene defect; carrying out mating on homozygotes and heterozygotes in offspring; and carrying out mating on male and female homozygotes in the offspring to obtain a large number of homozygous mice with the IFN-gamma gene defect. Propagation of the homozygous mice with the IFN-gamma gene defect is achieved; and meanwhile, the obtained homozygous mice with the IFN-gamma gene defect have more normal constitution than directly purchased homozygous mice with the IFN-gamma gene defect, can be better applied to establishment of a model for cryptosporidium infection in vitro, can cause rapid chronic infection at very low doses, are low in mortality in the process, and can meet the requirement for a relatively long research cycle for cryptosporidium infection. Furthermore, the homozygous mice with the IFN-gamma gene defect obtained by propagation can be directly subjected to homozygous-homozygous mating to generate the offspring, so that mass propagation is achieved and complex homozygous-heterozygous mating is not required any more.

Description

technical field [0001] The invention relates to the technical field of animal experiment model breeding, and more specifically, to a method for multiplying homozygous mice with IFN-γ gene deficiency and its application. Background technique [0002] Cryptosporidium is a single-celled parasite that can infect a variety of animals, including humans, and is a member of the apicomplexan phylum. Gastrointestinal disease caused by Cryptosporidium, known as cryptosporidiosis, is one of the common causes of diarrhea in children worldwide and can have long-term adverse effects on children's growth and development, as well as those with AIDS and the immunocompromised life-threatening. In 1907, the famous parasitologist Tyzzer found a large number of worms when he sliced ​​the mucous membrane of mice, and named it C. muris. In 1912, Tyzzer found similar worms in the small intestine of mice, but thought it was another worm species. , because this species is smaller than C. muris and t...

AI Technical Summary

Problems solved by technology

However, in the prior art, the purchased IFN-γ gene-deficient homozygous mice are generally used for infection experiments. Compared with normal mice, the directly purchased IFN-γ gene-deficient homozygous mice develop slower and have weaker constitutions. , cannot reproduce effectively, and may trigger a stress response when inoculating Cryptosporidium oocysts, resulting in the death of mice, or death due to weak constitution during the Cryptosporidium infection experiment, which cannot meet the long-term requirements for Cryptosporidium infection. Study Cycle Requirements

On the other hand, due to the need for a large number of IFN-γ gene-deficient homozygous mice for experiments, and the high price of homozygotes leads to high experimental costs

At present, there is no report on the successful expansion of IFN-γ gene-deficient homozygous mice

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