Open type bacteriostatic culture medium and preparation method thereof

An open, culture medium technology, applied in botany equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problems of high rate of bacterial and fungal contamination, poor antibacterial effect, etc., to achieve easy promotion, antibacterial The effect of good bacteria effect and simple operation process

Active Publication Date: 2020-05-12
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the general antibacterial agent has poor antibacterial effect, and the rate of bacterial contamination and fungal contamination is high.

Method used

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  • Open type bacteriostatic culture medium and preparation method thereof
  • Open type bacteriostatic culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. The bacteriostatic agent for the open antibacterial medium, which includes: cefixime 0.2g / L, streptomycin 0.2g / L, carbendazim 0.2g / L, and mancozeb 0.2g / L, the concentration is the final concentration when used.

[0020] 2. Open antibacterial medium: based on 1L of basic medium, add 2g of agar and 7g of starch to the basic medium, heat to dissolve, add sodium hypochlorite with a final concentration of 0.1ml / L when the temperature drops to 40°C, and the The antibacterial agent (0.2g cefixime, 0.2g streptomycin, 0.2g carbendazim, and 0.2g mancozeb);

[0021] 3. Inoculate the explants of Pinellia sinensis into the open antibacterial medium and cultivate them.

Embodiment 2

[0023] Bacteriostatic agents for open bacteriostatic medium, including: cefixime 0.1g / L, streptomycin 0.1g / L, carbendazim 0.1g / L, and mancozeb 0.1g / L , the concentration is the final concentration when used. All the other steps are the same as in Example 1.

Embodiment 3

[0025] Bacteriostatic agents for open bacteriostatic medium, including: cefixime 0.3g / L, streptomycin 0.3g / L, carbendazim 0.3g / L, and mancozeb 0.3g / L , the concentration is the final concentration when used. All the other steps are the same as in Example 1.

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Abstract

The invention discloses a bacteriostatic agent for an open type bacteriostatic culture medium, the open type bacteriostatic culture medium and a preparation method of the open type bacteriostatic culture medium. The bacteriostatic agent for the open type bacteriostatic culture medium comprises 0.1-0.3 g/L of cefixime, 0.1-0.3 g/L of streptomycin, 0.1-0.3 g/L of carbendazim and 0.1-0.3 g/L of mancozeb, wherein the concentration refers to the final concentration during use. The open type bacteriostatic culture medium comprises the bacteriostatic agent and also comprises a basic culture medium and a curing agent. The bacteriostatic agent has a good bacteriostatic effect, the whole operation process is simpler, the tissue culture cost is reduced, indoor operation can be allowed, and the accessthreshold is low. The operation is extensive, promotion in rural areas is facilitated, and mass production is facilitated.

Description

technical field [0001] The invention relates to the technical field of medium preparation, in particular to an open antibacterial medium and a preparation method thereof. Background technique [0002] The preparation of the traditional culture medium is cumbersome, and requires samples of a large number of elements, trace elements, organic and iron salts to be fixed and dissolved, and some hormones resistant to high temperature and high pressure are added to adjust the ph. The transfer of explants can only be carried out after disinfection and sterilization. The entire operation process is relatively cumbersome, energy-consuming, and requires high operating specifications. It is difficult for ordinary operators to meet the requirements. [0003] "Plant open tissue culture" is under the action of bacteriostatic agent, so that the plant tissue culture is separated from the strict aseptic operating environment, no high pressure sterilization and ultra-clean workbench are requir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张建李欣郭丽红赵振军王迪汪雅诗曹运梅杨朝东
Owner YANGTZE UNIVERSITY
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