Tissue culture inoculating method for dendrobium officinale

Abstract

The invention discloses a tissue culture inoculating method for dendrobium officinale. The tissue culture inoculating method for the dendrobium officinale comprises the following steps: selection of dendrobium officinale pods, sowing of seed embryos, preparation of sterile seed embryo mixed liquid, preparation of a germinating and rooting culture medium, and inoculation and culture of the seed embryos. The method disclosed by the invention is a simple and effective tissue culture inoculating method. According to the method, the germinating and rooting culture medium is inoculated to the cultured seed embryos through once induction, so that repeated culture rotation is not required; moreover, the germinating and rooting culture medium prepared in the method disclosed by the invention is mainly based on a natural antibacterial additive, and does not have harmful chemical component additive, so that the problems that the debugging time is long, the workload is long, the pollution rate is high, and the stability of produced seed seedlings is poor in conventional artificial one-by-one inoculation can be effectively solved; the pollution rate in the tissue culture process of the dendrobium officinale can be greatly reduced, and the tissue culture cost of the dendrobium officinale can be reduced; the tissue culture inoculating efficiency and the transplanting survival rate of the dendrobium officinale are greatly improved; the production efficiency is 3 to 4 times that of the conventional method; the transplanting survival rate can reach more than or equal to 92 to 93 percent.

Description

technical field [0001] The invention belongs to the technical field of plant bioengineering, and in particular relates to a tissue culture inoculation method of dendrobium officinale. Background technique [0002] Dendrobium officinale ( Dendrobium officinale ) is a perennial herb of the genus Dendrobium orchidaceae. Wild Dendrobium candidum is mainly distributed in Zhejiang, Anhui, Jiangxi, Guangxi and other places. The seeds of Dendrobium candidum have a unique structure, no endosperm, and the natural reproduction rate is extremely low. Coupled with people's frantic excavation of wild resources, wild resources of Dendrobium candidum are on the verge of extinction. With the development of modern biological tissue culture technology, the artificial breeding technology of Dendrobium officinale seedlings has gradually matured. At present, the industrialized Dendrobium officinale seedling breeding generally adopts the pods of Dendrobium officinale as the provenance. After the...

AI Technical Summary

Problems solved by technology

In the transfer process of single seedling transfer, strong seedling and rooting, it is necessary to manually transfer the seedlings one by one to the new medium with inoculation tweezers, which consumes a lot of labor and raw materials during the transfer process. , the process is cumbersome and inefficient, resulting in high production costs

In addition, during the transfer process, if the strength is not properly controlled, the seedlings will be physically damaged when the seedlings are clamped, resulting in slow growth of the seedlings, yellowing of the leaves, and overall chlorosis and death, which will seriously affect the survival rate of the seedlings.