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Regeneration culture medium for blueberry tissue culture as well as culture method and application of regeneration culture medium

A regeneration medium and tissue culture technology, applied in the fields of application, plant regeneration, horticultural methods, etc., can solve the problems of limited reproduction scale, low reproduction coefficient and emergence rate, and difficult rooting, and achieve high induction efficiency and increase induction rate , the effect of high differentiation rate

Active Publication Date: 2017-07-28
JINHUA VOCATIONAL TECH COLLEGE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the increasing demand for blueberries in the market recently, traditional propagation methods such as cuttings and layering have low reproduction coefficient and emergence rate, difficulty in rooting, and limited propagation scale, which is difficult to meet the needs of rapid promotion of fine varieties.

Method used

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  • Regeneration culture medium for blueberry tissue culture as well as culture method and application of regeneration culture medium
  • Regeneration culture medium for blueberry tissue culture as well as culture method and application of regeneration culture medium
  • Regeneration culture medium for blueberry tissue culture as well as culture method and application of regeneration culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1－4

[0142] A regeneration medium for blueberry tissue culture, including a primary medium, a proliferation medium and a leaf adventitious bud induction medium. The formulations of the medium in each stage of Examples 1-4 are shown in Table 1.

[0143] Table 1 embodiment 1-4 the formula of each stage culture medium

[0144]

[0145] Among them, the improved WPM medium includes element A liquid, element B liquid, element C liquid, element D liquid, element E liquid and organic matter;

[0146] The composition and content of element A liquid are: 400mg / L (NH 4 ) 2 SO 4 , 684mg / L calcium sugar alcohol;

[0147] The composition and content of element B liquid are: 6.2mg / L H 3 BO 3 , 170mg / L KH 2 PO 4 , 0.25mg / L NaMoO 4 2H 2 O;

[0148] The composition and content of element C liquid are: 190mg / L KNO 3 ;

[0149] The composition and content of element D liquid are: 370mg / L MgSO 4 ·7H 2 O, 22.3mg / L MnSO 4 ·H 2 O, 8.6mg / LZnSO 4 ·7H 2 O, 0.25mg / L CuSO4 ·5H 2 O;

[01...

experiment example 1

[0162] A culture method for in vitro regeneration of blueberry leaves, comprising the following steps:

[0163] (1) Selection and disinfection treatment of explants: select blueberry vigorous growth season strong, no disease and insect pests, good semi-lignified "Brigitta" shoots as explants, and carry out disinfection treatment on the explants;

[0164] The disinfection process is as follows: remove the leaves of the collected branches, keep the petioles, and cut them into 6cm stems with axillary buds; rinse the stems with running water for 30 minutes, soak them in a detergent solution with a mass concentration of 0.1% for 5 minutes, and then wash them with running water. Rinse with water for 30 minutes; then soak in alcohol with a volume ratio of 75% on the ultra-clean workbench for 20 seconds, then soak in sodium hypochlorite with a volume fraction of 30% for 30 minutes, rinse with sterile water for 3 times, each time for 2 minutes, drain the water, and set aside .

[0165...

experiment example 2－8

[0172] Different from Experimental Example 1, the blueberry tissue culture regeneration media used in Experimental Examples 2-8 are the regeneration media provided in Examples 1, 2, 4 and Comparative Examples 1-4 respectively.

[0173] The specific culture method is the same as that of Experimental Example 1.

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Abstract

The invention belongs to the technical field of tissue culture of plants, and particularly relates to a regeneration culture medium for blueberry tissue culture as well as a culture method and application. The regeneration culture medium for blueberry tissue culture comprises an initial culture medium, an enrichment culture medium and a leaf adventitious bud induction culture medium, wherein the initial culture medium is prepared from an improved WPM culture medium, 6-BA, saccharose and plant gel, and the pH is 5.2 to 5.4; the enrichment culture medium is prepared from an improved WPM culture medium, ZT, saccharose and plant gel, and the pH is 5.2 to 5.4; the leaf adventitious bud induction culture medium is prepared from an improved WPM culture medium, TDZ, saccharose and plant gel, and the pH is 5.2 to 5.4; and the improved WPM culture medium is obtained by replacing CaCl2.2H2O in a WPM culture medium with sugar alcohol calcium. The regeneration culture medium has the advantages of strong pertinence, high applicability and low cost, and can effectively increase the inductivity of a callus and the differentiation rate of adventitious buds.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a regeneration medium, a culture method and an application of blueberry tissue culture. Background technique [0002] Blueberry belongs to Rhododendronaceae Vaccinium, and it is an emerging fruit tree species with high economic value and broad development prospects. Blueberry fruit is rich in nutrition and can be eaten fresh or processed. It is low in energy and contains a variety of antioxidant components, especially its antioxidant capacity is the first among all fruits and vegetables. Blueberry fruit not only has the unique effect of enhancing human immunity, anti-cancer and anti-cancer, but also can significantly reduce the incidence of cardiovascular disease, diabetes, Parkinson's disease, etc.; therefore, blueberry is known as the "golden berry" (FAO) one of the five major health foods recommended by human beings. [0003] The planting and researc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 朱友银宗宇李永强陈文荣郭卫东刘向蕾梅淑芳应炎标
Owner JINHUA VOCATIONAL TECH COLLEGE
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