Culture medium for tissue culture of valeriana jatamansi and culture method

A technology of spider incense and culture medium, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of restricting the rapid scale of artificial cultivation, long breeding cycle, low reproductive rate, etc., and achieve high industrial promotion value, The effect of reducing the cost of tissue culture and improving the efficiency of tissue culture

Active Publication Date: 2019-01-18
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the continuous development and application of the pharmacological effects of spider incense and the development and application of daily chemical products and food, the market demand for spider incense is expanding day by day. However, spider incense is basically supplied to the market by mining wild resources, and limited wild resources have been over-harvested. Digging and the destruction of the ecological environment lead to the shortage of spider incense resources, which limits the application of the spider incense function. At present, only a small amount of cultivation has begun in Yunnan, Guizhou and other places, and the current main propagation method is to dig wild resources for division propagation
On the one hand, the total biomass of wild resources is limited. On the other hand, the reproductive coefficient of ramets is 2-5, and the reproductive rate is low. Generally, the reproductive cycle is 2-3 years. The long reproductive cycle limits the rapid scale of artificial cultivation in a short period of time. change

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Establishment of aseptic system: Take the underground rhizome of spider incense, rinse the soil with tap water, rinse with detergent solution for 30 minutes, rinse with tap water 3 times, scrape off the epidermis, rinse with tap water for 80 minutes, transfer to the ultra-clean table, use 75 Disinfected with% alcohol for 20 seconds, rinse with sterile water twice, disinfect with 100mg / L chlorine dioxide solution for 30 minutes, rinse with sterile water 3 times, disinfect with 0.1% mercury solution + 2 drops of Tween-80 for 10 minutes, rinse with sterile water Wash 7 times.

[0027] 2. Induction and cultivation of clump buds: Inoculate the detoxified subterranean rhizomes with buds of spider odorifera into modified MS+KT 5.0mg / L+IBA 0.01mg / L+active carbon 0.5g / L+white sugar 30g / L+agar 3.8g / L In the induction proliferation medium, the pH was adjusted to 5.8, the temperature was 25±1℃, the light intensity was 2500lx, and the single day light time was 12h for 60 days of alt...

Embodiment 2

[0033] 1. Establishment of aseptic system: Take the underground rhizome of spider incense, rinse the soil with tap water, rinse with detergent solution for 30 minutes, rinse with tap water 3 times, scrape off the epidermis, rinse with tap water for 60 minutes, transfer to the ultra-clean table, use 75 % Alcohol disinfection for 30 seconds, sterile water rinse for 3 times, 100mg / L chlorine dioxide solution disinfection for 30 minutes, sterile water rinse for 4 times, 0.1% mercury solution + 2 drops of Tween-80 disinfection for 15 minutes, sterile water rinse Wash 6 to 8 times.

[0034] 2. Induction and cultivation of clumping buds: Inoculate the detoxified subterranean rhizomes with buds of spider odorifera into modified MS+KT10.0mg / L+IBA 0.2mg / L+active carbon 0.5g / L+white sugar 30g / L+agar 3.8g / L In the induction proliferation medium, the pH was adjusted to 5.8, the temperature was 25±2°C, the light intensity was 2000lx, and the single day light time was 12h for 60 days of alterna...

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PUM

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Abstract

The invention discloses a culture medium for tissue culture of valeriana jatamansi and a culture method. The culture medium for the tissue culture has the advantages of simple components, low cost andeasiness for obtaining; the induction rate, multiplication coefficient and rooting rate of the valeriana jatamansi can be remarkably improved; the tissue culture efficiency can be effectively improved and the production method is reduced; the culture medium has market-oriented application value.

Description

Technical field [0001] The invention relates to the field of Chinese medicine tissue culture, in particular to a spider incense tissue culture medium and a culture method. Background technique [0002] Valeriana jatamansi Jones (Valeriana jatamansi Jones), also known as horseshoe incense, is a perennial root herb belonging to Valerian in the Patriniaceae family, and its rhizome is used as medicine. It has the effects of regulating qi and relieving pain, eliminating food and relieving diarrhea, dispelling wind and removing dampness, and calming nerves. It is mainly used for abdominal distension and pain, food accumulation, diarrhea, dysentery, rheumatic arthralgia, waist and knee pain, insomnia, etc. It has important medicinal value Resource plants. The volatile oil contained in spider incense has a special aroma. The main components are isovaleric acid, 3-methylvaleric acid, camphor, borneol, chlorophyllene, olivine, etc. It is used as an additive in cigarettes, and it has antib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 张金渝蒋元省赵露琴杨维泽杨绍兵曾祥飞邓清李纪潮余正强沈孝明苏杰
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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