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Simple tissue culture method for broussonetia papyrifera

A simple and tissue culture technology, applied in the field of plant tissue culture, can solve the problems of high manual operation cost, high energy consumption of aseptic disinfection, etc., and achieve the effect of reducing tissue culture cost, reducing workload and energy consumption, and simple operation

Inactive Publication Date: 2012-07-18
FUJIAN AGRI & FORESTRY UNIV
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AI Technical Summary

Problems solved by technology

Conventional mulberry tissue culture methods require the inoculation process and the culture process to be carried out in sterile medium, which consumes a lot of energy for aseptic disinfection and high manual operation costs

Method used

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Examples

Experimental program
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Embodiment 1

[0035] Embodiment 1: A kind of convenient tissue culture method of mulberry tree

[0036] A kind of convenient tissue culture method of mulberry tree, comprising the following steps:

[0037] 1. Preparation of bacteriostatic agent: The preparation method of bacteriostatic agent No. 1: add 20 ml of distilled water to the bottle of Eperon fungicide A to dilute it, mix it with the drug of bottle B, and dilute the volume to 100 ml with distilled water for antibacterial The preparation method of agent No. 1 and bacteriostatic agent No. 2: add 20 ml of distilled water to the bottle of Yibailong fungicide A to dilute it, mix it with the drug in bottle B, then add 15 ml of sodium hypochlorite solution, and dilute to 100 ml with distilled water. Bacteriostat No. 2;

[0038] 2. Disinfection of the culture container: Soak the culture bottle and bottle cap in an aqueous solution with a volume ratio of antibacterial agent No. 1 to water of 0.4% (V / V) for 10 hours, and save it for later us...

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Abstract

The invention relates to a simple tissue culture method for broussonetia papyrifera. The simple tissue culture method for the broussonetia papyrifera comprises the following steps: preparing a bacteriostatic agent, sterilizing a culture vessel, preparing a culture medium, performing induction culture, performing proliferation culture, performing rooting culture and transplanting a bottled plantlet. By the simple tissue culture method for the broussonetia papyrifera, the culture vessel and the culture medium are not required to be sterilized at high temperature and high pressure, so that the workload and the energy consumption are reduced, the tissue culture link of the broussonetia papyrifera is simplified, and tissue culture cost of the broussonetia papyrifera is lowered; the simple tissue culture method for the broussonetia papyrifera is easy to operate, high in practicality and good in popularization, and the culture medium is prepared according to different formulas and the bacteriostatic agent is added; and compared with the conventional tissue culture method for the broussonetia papyrifera, by the simple tissue culture method for the broussonetia papyrifera, the cost can be reduced by more than 10%.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a simple tissue culture method of mulberry tree, which belongs to the field of biotechnology. Background technique [0002] mulberry tree ( Broussonetia papyrifera L ) belongs to Moraceae, which is a perennial broad-leaved tree with the characteristics of fast growth, strong adaptability, wide distribution, high heat and short rotation period. Marigold mulberry is a special economic forest tree species with less investment, short cycle, quick effect and long benefit. It is mainly distributed in the Yellow River, Yangtze River and Pearl River basins in my country. The phloem fiber content of the mulberry tree is high, the fiber is long and thin, and the quality is good. It is a high-quality raw material for the production of rice paper and coinage paper; the leaves are rich in protein, amino acids, vitamins, carbohydrates and trace elements. It is a pure natural food for processin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 阙友雄林庆良许莉萍高世武
Owner FUJIAN AGRI & FORESTRY UNIV
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