Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of antibacterial agent for open tissue culture of Fritillaria sichuanensis and its application method

A tissue culture and bacteriostatic technology, which is applied in the field of bacteriostatic agents for open tissue culture of Fritillaria fritillary, can solve problems such as high cost and low survival rate, and achieves cost reduction, differentiation rate improvement, and broad-spectrum sterilization ability. Effect

Inactive Publication Date: 2016-04-20
SICHUAN AGRI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims at the above-mentioned deficiencies of the high cost and low survival rate of Fritillaria chinensis tissue culture in the prior art, and provides a kind of antibacterial agent for open tissue culture of Fritillaria chinensis, which has broad-spectrum bactericidal ability and It can protect the normal growth of plants and make the large-scale production of fritillaria seedlings possible

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of antibacterial agents:

[0039] The composition and content of antibacterial agent I are: antibacterial agent I with a total volume of 1000ml contains: loofah water 50mg, honeysuckle extract 10mg, mugwort leaf extract 10mg, cork extract 10mg, pepper extract 15mg, comfrey extract 15mg, 10mg of Coptis chinensis extract, 10mg of dandelion extract, 15mg of capillary root extract, 15mg of Daqingye extract, 100mg of amoxicillin-sulbactam, 70mg of PVP (polyvinylpyrrolidone), 0.7mg of chlorine dioxide, and 35mg of vitamin C;

[0040] The composition and content of antibacterial agent II are: antibacterial agent II with a total volume of 1000ml contains: 50mg of loofah water, 10mg of honeysuckle extract, 10mg of mugwort leaf extract, 15mg of Phellodendron phellodendri extract, 15mg of Zanthoxylum bungeanum extract, and comfrey extract 10mg, Coptis chinensis extract 5mg, Dandelion extract 10mg, Capillary wormwood extract 15mg, Daqingye extract 15mg, amoxicillin sulb...

Embodiment 2

[0057] Preparation of antibacterial agents:

[0058] The composition and content of bacteriostatic agent I are: bacteriostatic agent I with a total volume of 1000ml contains: 60mg of loofah water, 13mg of honeysuckle extract, 15mg of Artemisia argyi leaf extract, 12mg of Cortex Phellodendri extract, 10mg of Zanthoxylum bungeanum extract, and comfrey extract 12mg, 8mg of Coptis chinensis extract, 13mg of dandelion extract, 13mg of capillary root extract, 12mg of Daqingye extract, 130mg of amoxicillin-sulbactam, 150mg of PVP (polyvinylpyrrolidone), 4mg of chlorine dioxide, and 40mg of vitamin C;

[0059] The composition and content of antibacterial agent II are: antibacterial agent II with a total volume of 1000ml contains: 60mg of loofah water, 13mg of honeysuckle extract, 15mg of mugwort leaf extract, 12mg of Phellodendron phellodendri extract, 10mg of Zanthoxylum bungeanum extract, and comfrey extract 12mg, 8mg of Coptis chinensis extract, 13mg of dandelion extract, 13mg of c...

Embodiment 3

[0075] Preparation of antibacterial agents:

[0076] The composition and content of antibacterial agent I are: antibacterial agent I with a total volume of 1000ml contains: loofah water 70mg, honeysuckle extract 15mg, mugwort leaf extract 20mg, cork extract 15mg, pepper extract 5mg, comfrey extract 10mg, 5mg of Coptis chinensis extract, 15mg of dandelion extract, 10mg of capillary root extract, 12mg of Daqingye extract, 200mg of amoxicillin-sulbactam, 200mg of PVP (polyvinylpyrrolidone), 7mg of chlorine dioxide, and 50mg of vitamin C;

[0077] The composition and content of antibacterial agent II are: antibacterial agent II with a total volume of 1000ml contains: 70mg of loofah water, 15mg of honeysuckle extract, 20mg of mugwort leaf extract, 10mg of Phellodendron phellodendri extract, 5mg of Zanthoxylum bungeanum extract, and comfrey extract 15mg, 10mg of Coptidis extract, 15mg of Dandelion extract, 10mg of capillary extract, 10mg of Daqingye extract, 300mg of amoxicillin-sul...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a bacteriostatic agent for open type tissue culture of unibract fritillary bulb, and a using method of the bacteriostatic agent. The bacteriostatic agent comprises bacteriostatic agent I and bacteriostatic agent II, has broad-spectrum sterilization capability and is capable of protecting the normal growth of plants, so that the large-scale production of unibract fritillary bulb seedlings can be realized; after the bacteriostatic agent for the open type tissue culture of the unibract fritillary bulb is adopted, the problem of pollution caused by human error and environment in the traditional inoculation operation can be avoided, the pollution rate is greatly reduced, the cultivation success rate of the unibract fritillary bulb reaches 93%, the tissue culture cost is saved by about 60%, and the cost is greatly lowered.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacteriostatic agent for open tissue culture of Fritillaria sichuanensis and a method for using the bacteriostatic agent to carry out open tissue culture. Background technique [0002] Fritillaria fritillary belongs to Liliaceae, mainly distributed in the southeastern margin of the Qinghai-Tibet Plateau, western Sichuan, Diqing Prefecture, Yunnan, Lijiang, Longnan, Gansu, northeastern and southern Tibet, and parts of Qinghai. Fritillaria sichuan is a good medicine for the treatment of exogenous wind-heat cough, chronic cough due to lung deficiency, less phlegm and dry throat; Sichuan is the authentic production area of ​​Fritillaria sichuan, and high-quality Fritillaria sichuan is mainly distributed in Ganzi Tibetan Autonomous Prefecture and Aba Tibetan Autonomous Prefecture. 3500-4500m high mountain and plateau area, the climate of this area is cold, mainly ethnic mino...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/36A01N3/00A01H4/00A01N59/00A01N43/90
Inventor 段晓宇唐敏叶萌张健
Owner SICHUAN AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com