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Open tissue culture method of polygonatum cyrtonema

A Polygonatum polyflora, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, to achieve the effect of reducing tissue culture costs and simplifying tissue culture links

Active Publication Date: 2021-11-23
ZHEJIANG SUB TROPICS CROP INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, open tissue culture has been successfully performed on dozens of plants, but Polygonatum polyflora open tissue culture has not been reported yet

Method used

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  • Open tissue culture method of polygonatum cyrtonema
  • Open tissue culture method of polygonatum cyrtonema
  • Open tissue culture method of polygonatum cyrtonema

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 refers to Figure 1-3 , an open tissue culture method of Polygonatum polyflora, comprising the steps of:

[0027] 1. Disinfection of the culture bag: the culture bag adopts a special self-supporting self-sealing bag (with a breathable film), after cleaning it with tap water, soak it with 0.1% sodium hypochlorite, press the culture bag with a heavy object and completely submerge it, soak for 2~ 3h. After soaking, pick up the culture bag, dry it, and filter the water.

[0028] 2. The configuration of the medium: prepare the basic medium according to the preparation method of the conventional medium, the basic medium is MS+0.3% sucrose+7.2% agar+6-BA1.0~3.0 mg / L+NAA0.2~0.5 mg / L, add different types of hormones and concentrations according to the needs of different growth stages of Polygonatum multiflora tissue culture, add water to constant volume, cover and boil for 2-3 minutes, adjust the pH to 5.8-6.0, add YJ104 bacteriostatic agent to inhibit bacteria ...

Embodiment 2

[0036] An open method for cultivating Polygonatum polyflora tissue culture seedlings, comprising the steps of:

[0037] In this embodiment, except the following operations are different, other operations are the same as those in Embodiment 1, and will not be repeated here.

[0038] The concentration of YJ104 antibacterial agent added in each medium was 40 mg / L.

Embodiment 3

[0040] An open method for cultivating Polygonatum polyflora tissue culture seedlings, comprising the steps of:

[0041] In this embodiment, except the following operations are different, other operations are the same as those in Embodiment 1, and will not be repeated here.

[0042] The concentration of YJ104 antibacterial agent added in each medium was 50 mg / L.

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Abstract

The invention relates to an open tissue culture method of polygonatum cyrtonema. According to the method, under the action of the bacteriostatic action of a YJ104 bacteriostatic agent and by addition of the YJ104 bacteriostatic agent into a polygonatum cyrtonema tissue culture medium, the growth and propagation of fungi and bacteria in the polygonatum cyrtonema tissue culture process are inhibited, so that the proliferation and rooting culture of the polygonatum cyrtonema under the bacterial condition are realized. The method is simple to operate, time-saving and labor-saving, greatly reduces the cost, and lays an important foundation for the development of an industrialized seedling production technology of the polygonatum cyrtonema.

Description

technical field [0001] The invention relates to an open tissue culture method of Polygonatum polyflora. Background technique [0002] Polygonatum cyrtonema (Polygonatum cyrtonema) is a perennial herb of the genus Polygonatum in the Liliaceae family. Polygonatum multiflora is a tonic medicinal material that is traditionally used for both medicine and food. With the continuous expansion of market demand, coupled with artificial overharvesting, its wild resources have decreased sharply, and it can no longer meet the growing market demand. Although artificial planting is one of the effective ways to solve the current shortage of Polygonatum multiflora resources, long-term artificial cultivation will also bring about the phenomenon of quality decline and aggravation of diseases and insect pests; difficulty. Therefore, constructing a tissue culture and rapid propagation system of Polygonatum multiflora and improving the reproduction coefficient is an effective way to solve the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 姜武陈家栋段晓婧陶正明
Owner ZHEJIANG SUB TROPICS CROP INST
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