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Anoectochilus formosanus sound seedling culture method

A cultivation method and technology of clematis, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of poor medicinal and health care effects, reduce processing difficulty, promote healthy and orderly development, and strengthen seedlings Obvious effect

Inactive Publication Date: 2018-06-01
容县明曦铁皮石斛种植场(微型企业)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of tissue culture of clematis, it is inevitable to produce a large number of discarded leaves and stems of clematis. These wastes are usually disposed of directly and harmlessly, and some merchants use them to dry and sell them as high-quality clematis products. , but its medicinal and health effects are extremely poor

Method used

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  • Anoectochilus formosanus sound seedling culture method
  • Anoectochilus formosanus sound seedling culture method
  • Anoectochilus formosanus sound seedling culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Take mature clematis seeds for disinfection treatment, rinse with tap water and detergent for 3 minutes, then disinfect with 75% alcohol for 30 seconds, then disinfect with 35% hydrogen peroxide for 10 minutes, and finally rinse with sterile water three times before sowing in the seed medium Protocorms are germinated in medium culture, and seedlings are obtained after protocorms are differentiated and proliferated.

[0036] Culture medium preparation: Wash the discarded clematis leaves and / or stems and homogenize them with a beater to obtain a slurry, take the agar and mix it with cold water, mix it with the above slurry and other components in the table below to make a culture medium base, the total amount is 10000ml. Divide the culture medium into culture bottles, 30ml per bottle. Then place the culture bottle containing the culture medium in a sterilizer for sterilization. After the sterilization is exhausted, the culture bottle is left to stand in a closed steriliz...

Embodiment 2

[0041] The difference from Example 1 is: 250000 mg / L of potatoes, 50000 mg / L of clematis leaves and / or stems, 25% of potatoes, and 5% of clematis leaves and / or stems.

Embodiment 3

[0043] The difference from Example 1 is: 180000mg / L of potato, 30000mg / L of clematis leaves and / or stems, 18% of potatoes, and 3% of clematis leaves and / or stems.

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Abstract

The invention discloses an anoectochilus formosanus sound seedling culture method. According to the anoectochilus formosanus sound seedling culture method, anoectochilus formosanus seedlings obtainedby tissue culture are transferred into a sound seedling culture medium and are subjected to sound seedling culture for 90 to 100 days; the sound seedling culture medium contains potatoes and waste anoectochilus formosanu leaves and / or stems. By adopting the anoectochilus formosanus sound seedling culture method, the effect of invigorating anoectochilus formosanus seedlings soundings can be remarkably improved and the medical property of anoectochilus formosanus also can be improved; circulating tissue culture is realized and the cultivation cost is reduced.

Description

technical field [0001] The invention relates to a method for cultivating strong seedlings of clematis. Background technique [0002] Clematis (also known as golden clematis) is a plant of the genus Orchidaceae. Among the folks, there are golden thread, golden earrings, black ginseng, golden thread tiger head banana, golden thread into the bone disappear, money grass, golden thread stone pine and so on. [0003] The seeds of clematis are tiny, consisting of immature embryos and several layers of seed coat cells, with low natural germination and reproduction rates. Previously, the supply of goods on the market mainly came from wild digging, and the supply of products has been in short supply. In recent years, with the in-depth research on its pharmacodynamics and clinical application, the understanding of the medicinal value of clematis has been further improved, the shortage of its market supply has become more severe, and wild resources have been continuously destructively ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 涂湘炎曾少兰吴华球
Owner 容县明曦铁皮石斛种植场(微型企业)
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