Open type method for cultivating toxin-free seedlings of sugarcanes

An open culture and sugarcane technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as difficult handling of substances and handling combinations, and achieve the goal of reducing the cost of tissue culture, simple operation, and simplifying the process of tissue culture. Effect

Inactive Publication Date: 2012-04-11
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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Problems solved by technology

It is difficult to find suitable treatment substances and treatment combinations for specific plant species

Method used

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  • Open type method for cultivating toxin-free seedlings of sugarcanes

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Embodiment 1

[0037] Embodiment 1, a kind of method of open culture sugarcane ROC22 virus-free seedling

[0038] A method for open cultivation of sugarcane ROC22 virus-free seedlings, comprising the following steps:

[0039] 1. Hot water treatment of seed stems: select plants without diseases and insect pests, take the double-bud stem section with full cane buds in the middle of the cane stem, wash it and soak it with 850 times diluted carbendazim for 30 minutes, wash it with clean water and put it in a constant temperature water bath , 50°C hot water treatment for 30 minutes, during which the cane stems were continuously stirred to make the cane stems heated evenly; then, the cane stems were taken out and covered with peat soil. under cultivation;

[0040] 2. Preparation of bacteriostatic agent: use Eperon fungicide to prepare bacteriostatic agent No. 1 and bacteriostatic agent No. 2; preparation method of bacteriostatic agent No. 1: add 10 ml of distilled water to the bottle of Eperon fu...

Embodiment 2

[0047] Embodiment two, a kind of method of open culture sugarcane FN15 virus-free seedlings

[0048] A kind of method of open cultivation sugarcane FN15 virus-free seedling comprises the following steps:

[0049] 1. Kind stem hot water treatment: refer to the kind stem hot water treatment of embodiment one;

[0050] 2. the preparation of antibacterial agent: the preparation of antibacterial agent with reference to embodiment one;

[0051] 3. Container disinfection: refer to the container disinfection of Example 1;

[0052] 4. Inoculation operation: refer to the inoculation operation of Example 1;

[0053] 5. Induction culture: refer to the induction culture in Example 1;

[0054] 6. Proliferation culture: inoculate the clustered buds induced by explants into MS+6-BA1.0 mg / L+KT 1.5 mg / L+bacteriostatic agent No. 2 3 ml / L+sucrose 30 g / L+agar powder 3.5 g / L for proliferation In the culture medium, transfer once every 15 days; the temperature of the culture room is 25-28 °C, th...

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Abstract

The invention relates to an open type method for cultivating toxin-free seedlings of sugarcanes, comprising the following steps: processing the stems with hot water; preparing the anti-bacterial agent; disinfecting the ware; inoculating; executing the inducing cultivation, the propagation cultivation and the rooting cultivation; and transplanting from seedling bottles. The open type method for cultivating the toxin-free seedlings of the sugarcanes of the invention needs not to sterilize the culture medium at high temperature, inoculates in a natural and open environment, does not need the clean benches, radically simplifies the cultivation ring, and reduces the cultivation cost. Compared with the traditional cultivation, the open cultivation at least reduces over 20% of cost. Because of simple operation, the method can be implemented after preparing the different culture mediums according to prescriptions and adding the anti-bacterial agent. The investment is little, the practicability is strong, and the popularization is good, so the method can be popularized in sugarcane planters.

Description

technical field [0001] The invention relates to a method for cultivating plant seedlings, in particular to an open method for cultivating virus-free sugarcane seedlings, which belongs to the field of biotechnology. Background technique [0002] Sugar is the four major agricultural products related to the national economy and the people's livelihood. Sucrose accounts for 92% of my country's total sugar production. Therefore, sucrose production is related to the safety of my country's sugar, and sucrose production depends on the production of raw sugarcane. The quality of seedlings directly affects the production and production of raw cane. quality. [0003] Sugarcane is a crop that uses the vegetative body-cane stem for asexual reproduction. The breeding cycle is as long as 10 years. Traditional seedlings carry a variety of pathogenic bacteria, including viral sugarcane mosaic virus, yellow leaf disease virus, etc., and bacterial Perennial root dwarf pathogens and white strea...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 林庆良许莉萍
Owner FUJIAN AGRI & FORESTRY UNIV
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