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Bacteriostatic composition for blueberry open tissue culture and preparation method thereof

A plant tissue culture and composition technology is applied in the field of bacteriostatic compositions to achieve the effects of reducing the pollution rate, reducing the cost of tissue culture, and simplifying the link of tissue culture

Inactive Publication Date: 2021-08-03
KUNMING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the deficiencies of the existing blueberry tissue culture methods, and aims to provide a safe, environment-friendly and non-toxic bacteriostatic composition for blueberry open tissue culture, which can effectively control the pollution problem in the culture process , reduce the cost of tissue culture, simplify the process of tissue culture, and improve the quality of blueberry seedlings

Method used

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  • Bacteriostatic composition for blueberry open tissue culture and preparation method thereof
  • Bacteriostatic composition for blueberry open tissue culture and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 prepares antibacterial composition

[0030] Weigh each component (unit: g) according to the following mass: garlic 8, belladonna 12, Tongcheng tiger 13; the preparation method is as follows: after the weighed garlic is ground, add 100mL of water to mix and filter with gauze, 4000r / min Centrifuge in a centrifuge for 5 minutes, and take the supernatant to obtain allicin; mix the weighed belladonna and tiger sage, add 200 mL of water to decoct for 1 hour, and take the filtrate for later use; mix the obtained two liquids evenly to obtain the antibacterial composition.

Embodiment 2

[0031] Embodiment 2 prepares antibacterial composition

[0032] Each component was weighed according to the following mass (unit: g): garlic 10, belladonna 15, Tongcheng tiger 9; the preparation method was the same as in Example 1.

Embodiment 3

[0033] Embodiment 3 prepares antibacterial composition

[0034] Each component was weighed according to the following mass (unit: g): Garlic 6, Belladonna 11, Tongcheng Tiger 15; the preparation method was the same as in Example 1.

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Abstract

The invention belongs to the field of antibacterial agents for blueberry open plant tissue culture. Specifically, it relates to an antibacterial composition for blueberry open plant tissue culture and a preparation method thereof. The present invention first finds through the component screening test that the antibacterial composition obtained by mixing garlic, belladonna and tongcheng tiger together can not only effectively and stably inhibit bacteria, but also significantly reduce the pollution rate, promote root growth, increase the number of test tubes Seedling height and fresh weight. The present invention further finds that the proportioning of garlic, belladonna and Tongcheng tiger according to the mass ratio of 6-10:11-15:9-15 has a better synergistic effect, especially according to the ratio of 8:12:13 After the proportioning, the three have a very significant synergistic effect, which can effectively control the pollution problem in the traditional tissue culture process, reduce the cost of tissue culture, simplify the tissue culture process, and improve the quality of seedlings.

Description

technical field [0001] The invention relates to an antibacterial composition applied to blueberry open tissue culture, and further relates to a preparation method of the antibacterial composition, which belongs to the field of antibacterial agents for blueberry open plant tissue culture. Background technique [0002] Blueberry (Blueberry), also known as lingonberry, is a shrub fruit tree of Ericaceae (Ericaceae) genus Vaccinium spp. The fruit is a small blue-purple berry covered with a layer of white fruit powder. It is rich in various vitamins and trace elements needed by the human body. Its nutritional value is much higher than that of ordinary fruits. In recent years, the social demand for blueberries has been increasing day by day. The supply of blueberry seedlings cannot meet the market demand. The slow speed of conventional seedling propagation technology and the easy degradation of varieties have become the main restrictive factors for industrial development. [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 陈泽斌靳松夏体渊苏源余磊徐胜光王定康景贵鹏杨亮
Owner KUNMING UNIV
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