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A kind of rapid tissue culture method of wolfberry black fruit

A black fruit wolfberry and tissue culture technology, which is applied in the field of plant tissue culture, can solve the problems of explant tissue culture for detailed description, involving tissue culture, etc., and achieve the effects of alleviating resource shortage, shortening the cycle, and improving efficiency

Inactive Publication Date: 2016-08-17
四川天府贝瑞生态农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Haoren Taben et al. (2005) used the stem segment with axillary buds to differentiate and root Lycium barbarum and callus to induce adventitious bud proliferation. They only involved tissue culture and did not study its rapid culture; patent application number 201310094393.2 involves a method for rapid propagation of high-quality Lycium barbarum seedlings. It is necessary to select high-quality Lycium barbarum seeds, disinfect with 75% alcohol, and rinse with sterile water for 3 to 4 times; then disinfect with 0.1% mercury chloride, and rinse with sterile water for 3 After ~4 times, inoculate the seeds in MS medium to cultivate aseptic seedlings. After culturing for 1 month, cut off the stems of the aseptic seedlings, cut them into 0.5cm long and short stems, and insert them into MS+KH 2 PO 4 13.5mg / L+NAA0.1mg / L medium to continue culture, no detailed instructions on tissue culture of explants

Method used

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  • A kind of rapid tissue culture method of wolfberry black fruit
  • A kind of rapid tissue culture method of wolfberry black fruit
  • A kind of rapid tissue culture method of wolfberry black fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: a kind of fast tissue culture method of Lycium barbarum, it comprises the following steps:

[0031] (1) Sterilization of explants: Take the young stems and stem tips of the adult black fruit Lycium barbarum branches and wash them with running water for 3 minutes, sterilize them with 75% alcohol for 7 seconds, wash them with sterile water for 3 times, and then wash them with 0.1% mercuric chloride. The solution was sterilized for 13 minutes, and washed 4 times with sterile water;

[0032] (2) Induction culture: Inoculate the sterilized young stem segments and shoot tips into MS + 0.5mg / L 6-BA + 0.1mg / LNAA medium, under the light intensity of 1500LX, and the light time of 16h / day, Cultivate in a culture room at 24°C for 25 days, and the axillary buds will elongate to 3cm on average;

[0033] (3) Rooting culture: Cut off the axillary buds, inoculate them into 1 / 2MS + 0.1mg / L NAA medium, and cultivate them in a culture room with a light intensity of 3000LX, a...

Embodiment 2

[0037] Embodiment 2: a kind of fast tissue culture method of Lycium barbarum, it comprises the following steps:

[0038] (1) Sterilization of explants: Take the young stems and shoot tips of the adult black fruit Lycium barbarum branches and wash them with running water for 5 minutes, sterilize them with 75% alcohol for 4 seconds, wash them twice with sterile water, and then wash them with 0.1% mercuric chloride The solution was sterilized for 17 minutes, and washed 6 times with sterile water;

[0039] (2) Induction culture: inoculate the sterilized young stem segments and shoot tips into MS + 0.5mg / L 6-BA + 0.1mg / LNAA medium, under the light intensity of 1500LX, and the light time of 12h / day, Cultivate in a culture room at 26°C for 45 days, and the axillary buds will elongate to 5cm on average;

[0040] (3) Rooting culture: Cut off the axillary buds, inoculate them into 1 / 2MS + 0.1mg / L NAA medium, and cultivate them in a culture room with a light intensity of 3000LX, a light...

Embodiment 3

[0044] Embodiment 3: a kind of fast tissue culture method of Lycium barbarum, it comprises the following steps:

[0045] (1) Sterilization of explants: Take the young stems and shoot tips of the adult black fruit Lycium barbarum branches and wash them with running water for 4 minutes, sterilize them with 75% alcohol for 5 seconds, wash them twice with sterile water, and wash them with 0.1% mercuric chloride The solution was sterilized for 14 minutes, and washed 5 times with sterile water;

[0046] (2) Induction culture: Inoculate the sterilized young stem segments and shoot tips into MS + 0.5mg / L 6-BA + 0.1mg / LNAA medium, under the light intensity of 1500LX, and the light time of 14h / day, Cultivate in a culture room at 25°C for 35 days, and the axillary buds will elongate to 4cm on average;

[0047] (3) Rooting culture: Cut off the axillary buds, inoculate them into 1 / 2MS + 0.1mg / L NAA medium, and cultivate them in a culture room with a light intensity of 3000LX, a light time...

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Abstract

The invention discloses a rapid tissue culture method for lycium ruthenicum murr, belonging to the technical field of plant tissue culture. The rapid tissue culture method comprises the following steps: explants sterilization, induction culture, rooting culture, subculture, acclimatization and transplantation. According to the rapid tissue culture method, the rooting culture can be carried out during the subculture, so that the tissue culture is high, the operation is simple and convenient, the period is short, and complete plants can be rapidly acquired; the tissue culture process is not influenced by environmental factors.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a tissue culture method, in particular to a rapid tissue culture method of wolfberry black fruit. Background technique [0002] Lycium ruthenicum Murr (Lycium ruthenicum Murr.) is a plant of the genus Lycium in the family Solanaceae, a halophytic medicinal plant, and a thorny shrub. Black fruit wolfberry is sweet and flat in nature. It is not only rich in protein, wolfberry polysaccharides, amino acids, vitamins, minerals, trace elements and other nutrients, but also rich in black fruit pigments - natural proanthocyanidins. Among them, red fruit wolfberry does not contain proanthocyanidins. The proanthocyanidin content of wolfberry blackberry exceeds that of blueberry, and it is the natural wild plant with the highest proanthocyanidin content found so far. At the same time, Lycium barbarum has many advantages such as drought resistance, cold resistance, saline-alkali ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 丁龙梅栗丹晏强罗丽君
Owner 四川天府贝瑞生态农业有限公司
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