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A kind of sweet potato chemical disinfection tissue culture method

A chemical disinfection and tissue culture technology, applied in the biological field, can solve the problems of high manual operation cost, high cost, high energy consumption, etc., and achieve the effect of reducing workload and energy consumption, strong practicability and simple operation.

Inactive Publication Date: 2017-01-11
FUJIAN AGRI & FORESTRY UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the conventional sweet potato stem tip tissue culture is carried out under sterile conditions, corresponding facilities and equipment and conventional consumables need to be configured in each link of the tissue culture, the cost is high, and the energy consumption is large. At the same time, the whole process must be performed under sterile conditions. Lower operation, high cost of manual operation

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  • A kind of sweet potato chemical disinfection tissue culture method
  • A kind of sweet potato chemical disinfection tissue culture method

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Embodiment 1

[0033] Embodiment 1: A kind of sweet potato chemical disinfection tissue culture method

[0034] A chemical disinfection tissue culture method for sweet potato, comprising the following steps:

[0035] 1. Disinfection of the culture container: Soak the culture bottle, bottle cap and stainless steel plate for inoculation in an aqueous solution of 100 mg / L sodium hypochlorite + 10 mg / L nisin + 100 mg / L calcium propionate for not less than 10 hours, and save for later use;

[0036]2. Medium preparation: shoot tip induction medium is MS+0.5mg / L 6-BA+0.2mg / L IAA+50mg / L sodium hypochlorite+1.5mg / L penicillin+20mg / L calcium propionate+20g / L sucrose +3.5g / L agar powder, pH5.8; proliferation medium: 1 / 2MS+50mg / L sodium hypochlorite+1.5mg / L penicillin+20mg / L calcium propionate+20g / L sucrose+3.5g / L agar powder , pH5.8; rooting medium is 1 / 2MS+0.5mg / L NAA+50mg / L sodium hypochlorite+1.5mg / L penicillin+20mg / L calcium propionate+20g / L sucrose+3.5g / L agar powder, pH5 .8; when preparing the ...

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Abstract

The invention relates to a chemical disinfection tissue culture method for Ipomoea batatas Lam.. The method comprises the following steps: culturing container disinfection, medium preparation, stem tip induction culturing, multiplication culturing, rooting culturing, and bottle seedling transplanting. According to the method, chemical reagents are used for achieving the aim of fungi killing or fungi inhibition; in the process of preparing culture media, culture containers and the culture media do not need to be subjected to high-temperature and high-pressure disinfection, so that the work amount and the energy consumption can be reduced, and the step of sweet potato tissue culture is simplified; the operation is easy as the media are prepared according to different media formulas; the practicability is high, the generalization performance is high, and the sweet potato tissue culture cost can be effectively reduced and can be reduced by 10% or more generally.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a sweet potato chemically sterilized tissue culture method, which belongs to the field of biotechnology. Background technique [0002] Sweet potato (Ipomoea batatas Lam.) is an annual herb of Convolvulaceae, which is a kind of vegetative propagation crop. It is widely planted in our country. So far in my country's southern provinces such as Guangdong, Fujian and other provinces, it is still the second largest food crop after rice. Sweet potatoes contain all seven types of nutrients necessary for the human body, including carbohydrates, proteins, vitamins, and dietary fiber, especially rich in dietary fiber, carotene, vitamins A, B, C, E, and potassium, iron, copper, selenium, and calcium. And more than 10 kinds of trace elements, the nutritional value is very high, and it is called the health food with the most balanced nutrition by nutritionists. It is also recommended by the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 袁照年林庆良
Owner FUJIAN AGRI & FORESTRY UNIV
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