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Open-type culture method for apple dwarfing stock tissue culture seedlings at rooting stage and culture medium adopted by method

An open culture and rooting medium technology, which is applied to the open culture method and the medium used in the rooting stage of apple dwarf rootstock tissue culture seedlings, can solve the problems of detachment, aseptic environment, pollution and the like in the tissue culture process, and achieves the Improve the efficiency of tissue culture, facilitate transportation, and simplify the effect of tissue culture

Inactive Publication Date: 2019-06-18
北京市海淀区植物组织培养技术实验室 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It solves the pollution problem, makes the tissue culture process out of the strict aseptic environment, fundamentally simplifies the tissue culture process, and it is completely possible to conduct open tissue culture, but the key is to find a bacteriostatic agent that can be added to the medium

Method used

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  • Open-type culture method for apple dwarfing stock tissue culture seedlings at rooting stage and culture medium adopted by method
  • Open-type culture method for apple dwarfing stock tissue culture seedlings at rooting stage and culture medium adopted by method
  • Open-type culture method for apple dwarfing stock tissue culture seedlings at rooting stage and culture medium adopted by method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Open culture method of apple dwarf rootstock tissue culture seedling rooting stage:

[0026] (1) select apple dwarf rootstock P3 tissue culture seedling and apple dwarf rootstock MARK tissue culture seedling;

[0027] (2) the apple dwarf rootstock P3 tissue culture seedling that step (1) is selected and the apple dwarf rootstock MARK tissue culture seedling are transferred to the rooting medium that contains 0.01% fairy water and carry out rooting culture; The composition is 1 / 2MS with IBA2.0mg / L, sugar 30g / L and agar 6g / L; the rooting medium is not sterilized at high temperature; the container for the rooting medium is an aviation cup.

[0028] See the open culture growth of apple dwarf rootstock P3 tissue culture seedlings figure 1 shown. See the open culture growth of apple dwarf rootstock MARK tissue culture seedlings figure 2 shown.

Embodiment 2

[0029] Example 2: Open culture method of apple dwarf rootstock tissue culture seedling rooting stage:

[0030] The process of the present embodiment is the same as that of embodiment 1, the difference is that the concentration of fairy water is 0.015%.

[0031] See the open culture growth of apple dwarf rootstock P3 tissue culture seedlings image 3 shown. See the open culture growth of apple dwarf rootstock MARK tissue culture seedlings Figure 4 shown.

[0032] The open culture method of the present invention and the beneficial effects of the medium used are further described by specific test examples below.

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Abstract

The invention discloses an open-type culture method for apple dwarfing stock tissue culture seedlings at the rooting stage and a culture medium adopted by the method, and belongs to the technical field of plant tissue culture. The culture method comprises the steps of (1) selecting apple dwarfing stock P3 tissue culture seedlings and apple dwarfing stock MARK tissue culture seedlings; (2) transferring the apple dwarfing stock P3 tissue culture seedlings and the apple dwarfing stock MARK tissue culture seedlings both of which are selected in step (1) into a rooting culture medium containing 0.01-0.015% of Shenxian water for rooting culture, wherein the culture temperature is 25 + / - 2 DEG C, and the illumination time is 12 hours per day, and the rooting culture medium is formed by adding 2.0mg / L IBA, 30 g / L sugar and 6 g / L agar into 1 / 2 MS and not sterilized at high temperature. The method has the following advantages that by adding the bacteriostatic agent Shenxian water into the rooting culture medium, the normal growth of the tissue culture seedlings is ensured, the functions of sterilization and bacterial resistance are achieved, and no harm can be caused to the rooting processof the tissue culture seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to an open culture method and a culture medium used in the rooting stage of apple dwarf rootstock tissue culture seedlings. Background technique [0002] The complicated technical links in the traditional tissue culture process are all formulated around pollution prevention. Pollution control has become the primary technology in tissue culture. Failure to reduce the pollution rate to an acceptable range means that the process cannot continue , and there are many factors that affect pollution, such as the type of explant, the season, time, and pretreatment method of the material, the type and concentration of the disinfectant, the disinfection time and disinfection method, the sterilization of the medium and the vessel, and the operator. , working environment requirements, etc. are closely related to pollution. Therefore, the pollution problem is very complic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘兰英张秦张军民李春玲
Owner 北京市海淀区植物组织培养技术实验室
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