A kind of open tissue culture method of Polygonatum polyflora

A Polygonatum polyflora, tissue culture technology, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, to achieve the effect of simplifying tissue culture links and reducing tissue culture costs

Active Publication Date: 2022-05-17
ZHEJIANG SUB TROPICS CROP INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, open tissue culture has been successfully performed on dozens of plants, but Polygonatum polyflora open tissue culture has not been reported yet

Method used

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  • A kind of open tissue culture method of Polygonatum polyflora
  • A kind of open tissue culture method of Polygonatum polyflora
  • A kind of open tissue culture method of Polygonatum polyflora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 refers to Figure 1-3 , an open tissue culture method of Polygonatum polyflora, comprising the steps of:

[0027] 1. Disinfection of the culture bag: the culture bag adopts a special self-supporting self-sealing bag (with a breathable film), after cleaning it with tap water, soak it with 0.1% sodium hypochlorite, press the culture bag with a heavy object and completely submerge it, soak for 2~ 3h. After soaking, pick up the culture bag, dry it, and filter out the water.

[0028] 2. The configuration of the medium: prepare the basic medium according to the preparation method of the conventional medium, the basic medium is MS+0.3% sucrose+7.2% agar+6-BA1.0~3.0 mg / L+NAA0.2~0.5 mg / L, add different types of hormones and concentrations according to the needs of different growth stages of Polygonatum multiflora tissue culture, add water to constant volume, cover and boil for 2-3 minutes, adjust the pH to 5.8-6.0, add YJ104 bacteriostatic agent to inhibit bacter...

Embodiment 2

[0036] An open method for cultivating Polygonatum polyflora tissue culture seedlings, comprising the steps of:

[0037] In this embodiment, except the following operations are different, other operations are the same as those in Embodiment 1, and will not be repeated here.

[0038] The concentration of YJ104 antibacterial agent added in each medium was 40 mg / L.

Embodiment 3

[0040] An open method for cultivating Polygonatum polyflora tissue culture seedlings, comprising the steps of:

[0041] In this embodiment, except the following operations are different, other operations are the same as those in Embodiment 1, and will not be repeated here.

[0042] The concentration of YJ104 antibacterial agent added in each medium was 50 mg / L.

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Abstract

The invention relates to an open tissue culture method of Polygonatum polyflora, which utilizes the bacteriostatic effect of YJ104 bacteriostatic agent and adds YJ104 antibacterial agent to the tissue culture medium of Polygonatum polyflora to achieve inhibition of Polygonatum polyflora tissue culture process. The growth and reproduction of fungi and bacteria, so as to realize the proliferation and rooting culture of Polygonatum multiflora under the condition of bacteria. The method has the advantages of simple operation, time-saving and labor-saving, greatly reduced cost, and has laid an important foundation for the development of the industrialized seedling raising technology of Polygonatum multiflora.

Description

technical field [0001] The invention relates to an open tissue culture method of Polygonatum polyflora. Background technique [0002] Polygonatum cyrtonema (Polygonatum cyrtonema) is a perennial herb of the genus Polygonatum in the Liliaceae family. Polygonatum multiflora is a tonic medicinal material that is traditionally used for both medicine and food. With the continuous expansion of market demand, coupled with artificial overharvesting, its wild resources have decreased sharply, and it can no longer meet the growing market demand. Although artificial planting is one of the effective ways to solve the current shortage of Polygonatum multiflora resources, long-term artificial cultivation will also bring about the phenomenon of quality decline and aggravation of diseases and insect pests; difficulty. Therefore, constructing a tissue culture and rapid propagation system of Polygonatum multiflora and improving the reproduction coefficient is an effective way to solve the s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 姜武陈家栋段晓婧陶正明
Owner ZHEJIANG SUB TROPICS CROP INST
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