A kind of open tissue culture method of Polygonatum polyflora
A Polygonatum polyflora, tissue culture technology, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, to achieve the effect of simplifying tissue culture links and reducing tissue culture costs
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Embodiment 1
[0026] Embodiment 1 refers to Figure 1-3 , an open tissue culture method of Polygonatum polyflora, comprising the steps of:
[0027] 1. Disinfection of the culture bag: the culture bag adopts a special self-supporting self-sealing bag (with a breathable film), after cleaning it with tap water, soak it with 0.1% sodium hypochlorite, press the culture bag with a heavy object and completely submerge it, soak for 2~ 3h. After soaking, pick up the culture bag, dry it, and filter out the water.
[0028] 2. The configuration of the medium: prepare the basic medium according to the preparation method of the conventional medium, the basic medium is MS+0.3% sucrose+7.2% agar+6-BA1.0~3.0 mg / L+NAA0.2~0.5 mg / L, add different types of hormones and concentrations according to the needs of different growth stages of Polygonatum multiflora tissue culture, add water to constant volume, cover and boil for 2-3 minutes, adjust the pH to 5.8-6.0, add YJ104 bacteriostatic agent to inhibit bacter...
Embodiment 2
[0036] An open method for cultivating Polygonatum polyflora tissue culture seedlings, comprising the steps of:
[0037] In this embodiment, except the following operations are different, other operations are the same as those in Embodiment 1, and will not be repeated here.
[0038] The concentration of YJ104 antibacterial agent added in each medium was 40 mg / L.
Embodiment 3
[0040] An open method for cultivating Polygonatum polyflora tissue culture seedlings, comprising the steps of:
[0041] In this embodiment, except the following operations are different, other operations are the same as those in Embodiment 1, and will not be repeated here.
[0042] The concentration of YJ104 antibacterial agent added in each medium was 50 mg / L.
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