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Culture method for prolonging storage period of kiwi fruit tissue culture seedlings

A culturing method and a technology for tissue culture seedlings are applied in the field of culturing for prolonging the preservation period of kiwifruit tissue culture seedlings, which can solve the problems of increasing the probability of culture material variation, costing a lot of manpower, material resources and time, and being unfavorable for the preservation of original germplasm, and reducing the Nutrient consumption, high application value, easy operation effect

Active Publication Date: 2019-03-08
POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Kiwifruit tissue culture seedlings are generally subcultured once a month, and can be stored for up to 2 months. Frequent subculture requires a lot of manpower, material resources and time; at the same time, as the number of subcultures increases, the probability of variation of culture materials also increases. increase, which is not conducive to the preservation of the original germplasm

Method used

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  • Culture method for prolonging storage period of kiwi fruit tissue culture seedlings

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Embodiment 1

[0020] A culture method for prolonging the shelf life of kiwifruit tissue culture seedlings, comprising the following steps:

[0021] (1) According to MS+6-BA 1.0 mg / L+NAA 0.5 mg / L+GA 3 0.1 mg / L+ agar 7g / L+ sucrose 15 g / L to prepare a special subculture medium; adjust the pH to 6.0 before the medium is sterilized;

[0022] (2) Select non-polluted, vigorously growing kiwifruit tissue culture seedlings to inoculate in the above medium;

[0023] (3) The inoculated kiwifruit tissue culture seedlings were stored in a culture room with a culture temperature of 25±1°C, a light time of 12 h / d, and a light intensity of 1500-2000 lx;

[0024] (4) The delayed-growing tissue-cultured seedlings can be kept for 5 months. During the period, when the leaves of the tissue-cultured seedlings are found to be dry, replace the new medium in time. The survival rate of the cultured tissue culture seedlings reached 86%.

Embodiment 2

[0026] A culture method for prolonging the shelf life of kiwifruit tissue culture seedlings, comprising the following steps:

[0027] (1) According to MS+6-BA 1.0 mg / L +NAA 1.0 mg / L+GA 3 0.1 mg / L+ agar 7g / L+ sucrose 20 g / L to prepare a special subculture medium; adjust the pH to 6.0 before the medium is sterilized;

[0028] (2) Select non-polluted, vigorously growing kiwifruit tissue culture seedlings to inoculate in the above medium;

[0029] (3) The inoculated kiwifruit tissue culture seedlings were stored in a culture room with a culture temperature of 25±1°C, a light time of 12 h / d, and a light intensity of 1500-2000 lx;

[0030] (4) The delayed-growing tissue-cultured seedlings can be kept for 4.5 months. During this period, when the leaves of the tissue-cultured seedlings are found to be dry, replace the new medium in time. The survival rate of the cultured tissue culture seedlings reached 83%.

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Abstract

The invention discloses a culture method for prolonging the storage period of kiwi fruit tissue culture seedlings. A certain amount of sucrose is added on the basis of the conventional subculture medium of kiwi fruit tissue culture seedlings, so that the storage time of the tissue culture seedlings can be effectively prolonged. The problem of short storage period of the existing kiwi fruit tissueculture seedlings is solved. Through the storage of the kiwi fruit tissue culture seedlings performed according to the method provided by the invention, the growth of the tissue culture seedlings canbe generally delayed for 4 to 5 months; the survival rate of the tissue culture seedlings can reach 80 percent or higher.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture method for prolonging the storage period of kiwifruit tissue culture seedlings. Background technique [0002] Kiwi fruit is rich in nutrients and contains a variety of vitamins, fats and proteins. Among them, the content of vitamin C is 100mg-400mg / 100g, which is much higher than that of oranges, pears and apples. It is known as the "King of Vitamin C". In addition to its edible value, kiwifruit has beautiful and fragrant flowers, which also have high ornamental value. my country is the center of the distribution of kiwifruit resources. The germplasm resources are abundant and widely distributed, but they have been in the wild or semi-wild state for a long time. Due to the deterioration of the ecological environment, some species are already endangered, so they urgently need to be collected and preserved. [0003] Commonly used preservation met...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 高敏霞陈义挺冯新赖瑞联陈文光
Owner POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI
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