Antibacterial culture method for genetically-transformed tobacco

A technology of genetic transformation and cultivation method, which is applied in the field of antibacterial cultivation after genetic transformation of tobacco, can solve the problems of heavy workload and cumbersome work procedures, and achieve the effects of reducing workload, reducing tissue culture costs, and strong practicability

Inactive Publication Date: 2014-07-30
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

The above work procedures are cumbersome and heavy workload

Method used

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  • Antibacterial culture method for genetically-transformed tobacco

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Embodiment 1

[0035] Embodiment 1: A kind of antibacterial culture method after genetic transformation of tobacco

[0036] A bacteriostatic culture method after genetic transformation of tobacco, comprising the following steps:

[0037] 1. Screening and preparation of antibacterial agents: select 4 kinds of medicaments with 3 concentrations each, and use L 9 (3) 4 Arrange the orthogonal table, prepare the medium, inoculate the infected tobacco leaves, and count the pollution rate and material necrosis rate after 15 days. The results show that sodium hypochlorite is the main factor affecting the pollution of the medium and material necrosis. High concentration mainly causes material necrosis; the differences among the levels of the other three factors have not reached the significant level after the significance test, see Table 5.

[0038] Table 5 Effects of different agents and concentrations on tissue culture

[0039]

[0040] According to the K value in Table 5 and the size of the R...

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Abstract

The invention relates to an antibacterial culture method for genetically-transformed tobacco. The antibacterial culture method comprises the steps of: bacteriostat preparation; culture vessel sterilization; agrobacterium tumefacien transformation; culture medium preparation; co-culture; screening culture; rooting culture; resistive seedling detection. Due to the adoption of the antibacterial culture method for the genetically-transformed tobacco, provided by the invention, a culture vessel and a culture medium are both not needed to be sterilized at high temperature and high pressure, so that the workload and energy consumption are reduced, the culture link of the genetically-transformed tobacco is simplified, and the culture cost of the genetically-transformed tobacco is reduced; the antibacterial culture method for the genetically-transformed tobacco, provided by the invention, is simple in operation, strong in practicability and good in popularization, and only a bacteriostat is added after culture mediums are prepared according to different formulas; in addition, compared with the conventional method, the antibacterial culture method for the genetically-transformed tobacco can be used for reducing the cost by more than 10%.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a bacteriostatic culture method after genetic transformation of tobacco, which belongs to the field of biotechnology. Background technique [0002] With the rapid development of biotechnology and molecular biology, research on tobacco transgenics has become more and more in-depth and perfect. At present, research on genetically modified tobacco has improved the ability of tobacco to resist diseases, insect pests, adversity, herbicides, and heavy metal pollution, and has also played an important role in improving the quality and safety of tobacco. As tobacco is easy to carry out tissue culture, and it is easy to obtain regenerated and transformed tobacco plants, as a model plant, tobacco occupies a pivotal position in the study of biological functional genomes. [0003] Plant tissues, cells or protoplasts need to be screened after genetic transformation to distinguish transformant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84A01H5/00A01H4/00
Inventor 许莉萍林庆良高世武陈晓英
Owner FUJIAN AGRI & FORESTRY UNIV
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