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Special culture medium for improving tissue culture propagation speed of haworthia succulent plants and tissue culture method

A technology for succulents and culture medium, which is applied in the field of special medium for increasing the reproduction speed of tissue culture of succulents belonging to the genus Twelve succulents, can solve the problems of low reproduction rate of tissue culture and the like, so as to increase the reproduction speed, improve the speed of product launch, and reduce the The effect of tissue culture cost

Inactive Publication Date: 2015-11-04
泓柯(天津)农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of low tissue culture propagation rate in the above-mentioned background technology, the present invention provides a special medium for increasing the tissue culture propagation rate of Twelve succulents, the medium components are: basic medium and low Dosage of 2,4-D; the basal medium components are: MS20g, carrageenan 1g, 6-BA (6-benzylaminoadenine) 0.1mg, KT (chlorfenuron) 0.5mg

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Selection and disinfection of explants:

[0021] The leaves of the well-grown plants to be cultured are washed with clear water, soaked in a carbendazim solution with a mass content of about 5% for 10-15 minutes, and then rinsed with clear water.

[0022] Put 1% mercuric chloride solution in a sterile bottle on the ultra-clean workbench for 3-5 minutes (determine the length of soaking time according to the degree of tenderness and pollution), pour out the mercuric chloride solution, and use a cooled sterile Rinse with water 3-6 times, put into a sterile petri dish, cut into 1×1cm with a sterile blade 2 The size of the leaves, and scratch the back of the leaves, to be inoculated.

[0023] 2. Inoculation and establishment of sterile lines:

[0024] Place the cut leaves flat on the surface of the sterile culture medium with a sterile camera, touch the culture medium downward on the side that crosses the wound, tighten the mouth of the sterile medium bottle, mark it, a...

Embodiment 2

[0028] 1. Selection and disinfection of explants:

[0029] The leaves of the well-grown plants to be cultured are washed with clear water, soaked in a carbendazim solution with a mass content of about 5% for 10-15 minutes, and then rinsed with clear water.

[0030] Put 1% mercuric chloride solution in a sterile bottle on the ultra-clean workbench for 3-5 minutes (determine the length of soaking time according to the degree of tenderness and pollution), pour out the mercuric chloride solution, and use a cooled sterile Rinse with water 3-6 times, put into a sterile petri dish, cut into 1×1cm with a sterile blade 2 The size of the leaves, and scratch the back of the leaves, to be inoculated.

[0031] 2. Inoculation and establishment of sterile lines:

[0032] Place the cut leaves flat on the surface of the sterile culture medium with a sterile camera, touch the culture medium downward on the side that crosses the wound, tighten the mouth of the sterile medium bottle, mark it, a...

Embodiment 3

[0036] 1. Selection and disinfection of explants:

[0037] The leaves of the well-grown plants to be cultured are washed with clear water, soaked in a carbendazim solution with a mass content of about 5% for 10-15 minutes, and then rinsed with clear water.

[0038] Put 1% mercuric chloride solution in a sterile bottle on the ultra-clean workbench for 3-5 minutes (determine the length of soaking time according to the degree of tenderness and pollution), pour out the mercuric chloride solution, and use a cooled sterile Rinse with water 3-6 times, put into a sterile petri dish, cut into 1×1cm with a sterile blade 2 The size of the leaves, and scratch the back of the leaves, to be inoculated.

[0039] 2. Inoculation and establishment of sterile lines:

[0040] Place the cut leaves flat on the surface of the sterile culture medium with a sterile camera, touch the culture medium downward on the side that crosses the wound, tighten the mouth of the sterile medium bottle, mark it, a...

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Abstract

The invention provides a special culture medium for improving the tissue culture propagation speed of haworthia succulent plants and a tissue culture method. The special culture medium is characterized in that the ingredients of the special culture medium include a basic culture medium and low-dosage 2,4-D. The tissue culture method comprises the following steps of 1 explant selection and sterilization, 2 inoculation and aseptic strain establishment and 3 transfer and expanding propagation. The problem of low tissue culture propagation speed of the haworthia succulent plants can be solved, the propagation speed of the haworthia plants is remarkably improved, the cost of the whole tissue culture is reduced, and the marketing speed of products is improved.

Description

technical field [0001] The invention relates to the field of biological propagation, and mainly relates to a special culture medium and a tissue culture method for increasing the tissue culture propagation speed of succulents of the genus Twelve. Background technique [0002] Haworthia Dural is a perennial herbaceous small succulent plant belonging to the Aloe family of Liliaceae. It was named in honor of Adrian Hardn Haworth, a British botanist in the early 19th century. The genus is divided into Two types of hard leaves and soft leaves. Most of the plants of the genus Twelve are native to South Africa, and wild species are also distributed in the dry desert areas of our country, and are now widely planted all over the world. This genus is divided into about 20 lines, more than 150 varieties, and there are many horticultural varieties and hybrid varieties, and the price ranges from a few yuan to tens of thousands of yuan. The leaf shape of the genus Twelve is rich in chan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 夏时云韩伟丁亮
Owner 泓柯(天津)农业科技有限公司
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