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Blueberry tissue culture propagation and ex-vitro rooting method

A technology of external rooting and blueberry, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of low rooting rate of blueberry bottles, complicated rooting procedures, and long rooting cycle, so as to reduce the cost of tissue culture and improve The effect of survival rate and cycle shortening

Inactive Publication Date: 2014-06-25
HANGZHOU YOUGUO BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a method for blueberry tissue culture expansion and rooting outside the bottle, which mainly solves the problems of low rooting rate, long rooting cycle, complicated rooting procedure and high rooting cost when blueberry is rooted in a bottle. technical problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: a kind of blueberry tissue culture propagation of this example and the method for rooting outside the bottle, its steps are:

[0018] a. Selection and disinfection of explants: Select healthy plants without diseases and insect pests, cut 8-10cm semi-lignified branches, remove leaves, rinse under running water for 15-20 minutes, disinfect with 75% alcohol in the ultra-clean workbench for 30 seconds, and then without Rinse with sterile water for 2-3 times, transfer to 0.1% mercury liter for disinfection for 8 minutes, and rinse with sterile water for 3-4 times;

[0019] b. Induce lateral bud germination: Inoculate the sterile material obtained in the previous step into the medium for inducing lateral bud germination in the ultra-clean workbench. The medium components are: improved WPM basic medium + ZT 1.0mg / L + sugar 25g / L + agar 7g / L, pH adjusted to 5.4;

[0020] c. Proliferation culture: Cut the sterile lateral buds obtained in the previous step into ...

Embodiment 2

[0023] Embodiment 2: a kind of blueberry tissue culture propagation of this example and the method for rooting outside the bottle, its steps are:

[0024] a. Selection and disinfection of explants: Select healthy plants without diseases and insect pests, cut 8-10cm semi-lignified branches, remove leaves, rinse under running water for 15-20 minutes, disinfect with 75% alcohol in the ultra-clean workbench for 30 seconds, and then without Rinse with sterile water 2-3 times, transfer to 0.1% mercury liter for disinfection for 10 minutes, rinse with sterile water 3-4 times;

[0025] b. Induce lateral bud germination: Inoculate the sterile material obtained in the previous step into the medium for inducing lateral bud germination in the ultra-clean workbench. The medium components are: improved WPM basic medium + ZT 2.0mg / L + white sugar 30g / L + agar 8g / L, pH adjusted to 5.4;

[0026] c. Proliferation culture: Cut the sterile lateral buds obtained in the previous step into 2-3cm...

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Abstract

The invention relates to the technical field of industrialized seedling production and in particular relates to a blueberry tissue culture propagation and ex-vitro rooting method. The method mainly aims to solve the technical problems that rapid industrialized blueberry industrialized seedling production is restrained due to low rooting rate, long rooting period, complex rooting program, high rooting cost and the like during in-vitro blueberry rooting. The method comprises the following steps: selecting and sterilizing an explant, inducing lateral bud germination, performing multiplication culture, and finally performing ex-vitro rooting, wherein in the culture medium for inducing lateral bud germination, the components of the culture medium comprises an improved WPM basic culture medium, 0.8-2mg / L of ZT, 25-35mg / L of white sugar and 5-10g / L of agar; the pH value is regulated to be 5.4; according to the improved WPM basic culture medium, the original potassium sulfate and calcium chloride are replaced by calcium nitrate hydrate and potassium nitrate.

Description

technical field [0001] The invention relates to the technical field of industrial seedling cultivation, in particular to a method for blueberry tissue culture propagation and rooting outside a bottle. Background technique [0002] blueberry( Vaccinium spp. ), Rhododendron Vaccinium. The fruit is blue-purple, with unique flavor and rich nutrition. The nutritional content of blueberries is not limited to fructose, fiber, vitamins A and C, but more importantly, it is rich in compounds such as antioxidants, bacterial growth inhibitors, folic acid, anthocyanins, etc., which are effective in scavenging and inhibiting free radicals in the human body and reversing human aging. Therefore, it is also listed as one of the top five healthy foods for human beings by the International Food and Agriculture Organization. [0003] Blueberry not only has good international and domestic market prospects, but also can be unique in changing the structure of agricultural and forestry planting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 申雪颖
Owner HANGZHOU YOUGUO BIO TECH
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