Culture medium composition adapting for blueberry test tube plantlet proliferation as well as method thereof

Abstract

The invention relates to a culture medium composition suitable for the proliferation of blue berry plantlets and a method, comprising the following procedures: 1) the culture medium including a basic medium and tissue culture medium for each stage, the weight contained in each component per litre being: (1) the basic medium: WPM, wherein white sugar is 30g / L, agar is 7 to 8g / L, pH is 5.3; (2) induction medium: WPM+TDZ1.0 to 2.0mg / L+IBA0.3 to 0.5mg / L; (3) blade induction medium: WPM+TDZ2.0 to 3.0mg / L; (4) multiplication medium: WPM+TDZ0.2 to 0.5mg / L+IBA0.1 to 0.2mg / L+GA0.1mg / L; 2) selecting and sterilizing an explant; 3) inducting and culturing: directly inducing the initial generation of plumule from the explant; 4) culturing on the blade induction medium, forming embryogenic callus, or directly forming the regenerated adventitious buds having complete plants; 5) proliferating and culturing. The invention has the advantages that: 1) the culture medium has strong pertinence, good applicability and high induction rate of buds; 2) proliferation coefficient is high, and tissue culture cost is saved; 3) the genetic characters are the same, and the defect of low conventional multiplication coefficient is overcome.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and mainly relates to a culture medium composition suitable for the proliferation of blueberry test-tube seedlings and a method thereof. Background technique [0002] Blueberry (blueberry) is an emerging functional fruit. With its excellent quality, excellent processing performance and unique health functions, it has quickly become the third largest berry in the world, and has been listed as one of the five health foods for human beings by the International Food and Agriculture Organization. [0003] Blueberry fruit is low in energy and contains a variety of antioxidant ingredients, so it not only has the unique effect of anti-cancer and anti-cancer, but also can significantly reduce the incidence of cardiovascular disease, diabetes, Parkinson's disease, etc., and is also an important anti-aging, weight loss. beauty fruit. Blueberry is suitable for processing into processed products...

AI Technical Summary

Problems solved by technology

However, most of the existing literature reports use expensive zeatin for multiplication, which doubles the cost of tissue culture and makes it impossible to achieve large-scale industrial production