Method for preserving sargassum horneri fertilized eggs at low temperature

A technology for cryopreservation and fertilization of eggs, which is applied to the cultivation of economic brown algae, can solve problems such as difficulties in actual production, and achieve the effects of saving trouble, improving success rate, and delaying the development process.

Inactive Publication Date: 2014-11-19
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, this method will bring a lot of difficulties to actual production when it is necessary to complet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0020] 1) Collection of eggs and sperms from copper algae synchronously discharged: the male and female parents of copper algae were cultured in seawater under the conditions of light intensity of 50,000 lux, light cycle of 16 hours, and temperature of 18 degrees, and the water was changed once within 24 hours to cultivate 8 days, so that the parents realize the simultaneous discharge of eggs and sperm, and collect them within 24 hours after the parents release the eggs and sperm synchronously; the ratio of male to female individuals of the male and female parents of copper algae is 10:1.

[0021] 2) Preservation: Fertilize the collected synchronously released eggs and sperm, and the individual ratio of synchronously released eggs and sperm is 1:100. Collect the fertilized eggs within 2-3 days after fertilization, and store the collected fertilized eggs in seawater at a low temperature of 4°C and 0 lux; the collected fertilized eggs can be collected by natural sedimentation and...

Embodiment 3

[0023] 1) Collection of eggs and sperms from copper algae synchronously discharged: the male and female parents of copper algae were cultured in seawater under the conditions of light intensity of 30,000 lux, light cycle of 12 hours, and temperature of 18 degrees, and the water was changed once within 24 hours to cultivate After 10 days, the parents can release eggs and sperm synchronously, and they will be collected within 20 hours after the parents release eggs and sperm synchronously; the ratio of male and female individuals of the male and female copper algae parents is 10:1.

[0024] 2) Preservation: Fertilize the collected synchronously released eggs and sperm, and the individual ratio of synchronously released eggs and sperm is 1:50. Within 10 hours after fertilization, complete the collection of fertilized eggs at a low temperature of 1°C, and store the collected fertilized eggs at a low temperature of 1°C and 200 lux; the collected fertilized eggs can be collected by n...

Embodiment 4

[0026] 1) Collection of eggs and sperms released by copper algae synchronously: the male and female parents of copper algae were cultivated in seawater under the conditions of light intensity of 40,000 lux, light cycle of 10 hours, and temperature of 17 degrees, and the water was changed once within 24 hours. 6 days, so that the parents realize the simultaneous discharge of eggs and sperm, and collect them within 24 hours after the parents release the eggs and sperm synchronously; the ratio of male to female individuals of the male and female parents of copper algae is 10:1.

[0027] 2) Preservation: Fertilize the collected synchronously released eggs and sperm, and the individual ratio of synchronously released eggs and sperm is 1:8. The collection of fertilized eggs is completed within 14 hours after fertilization, and the collected fertilized eggs can be stored at a low temperature of 5°C and 50 lux at the same time; the collected fertilized eggs are collected by natural sed...

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Abstract

The invention relates to cultivation of economic brown seaweed and in particular relates to a method for preserving sargassum horneri fertilized eggs at low temperature. The method comprises the following steps: collecting eggs and sperms, which are synchronously discharged by economic seaweed of sargassum horneri, within 2-3 days after fertilization, and preserving the collected fertilized eggs in seawater at the temperature of 4-8 DEG C under 2000 lux. With the adoption of the method, the development process of the fertilized eggs can be greatly delayed, and trans-regional transport of the fertilized eggs in different places is finished before rhizoid elongation, so that seed production can be carried out.

Description

technical field [0001] The invention relates to the cultivation of economical brown algae, in particular to a method for preserving fertilized eggs of copper algae at low temperature. Background technique [0002] Copper algae is a dioecious economic brown algae. At present, the method of artificial seedling cultivation is usually adopted. This method uses mature parents to grow vegetables, or individuals from natural populations, or individuals cultivated artificially, or in In the artificial culture system, the male and female parents are matured synchronously through biotechnology, and the fertilized eggs are collected to complete the process of planting. These technical methods need to finish the cloth seedling process rapidly after collecting the fertilized eggs, so that the fertilized eggs can be attached to the attachment base before the elongation of the rhizome. Therefore, when it is necessary to complete cross-region seedling cultivation and transport a large amou...

Claims

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Application Information

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IPC IPC(8): A01N3/00
Inventor 逄少军李静单体锋
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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