The invention discloses an agricultural seedling cultivation device, which comprises an atomizer, a spraying pipe, supporting legs, a box body, a first seedling cultivation electromagnetic valve, a second seedling cultivation electromagnetic valve, a third seedling cultivation electromagnetic valve, a fourth seedling cultivation electromagnetic valve, a liquid supply pipe, a seedling cultivation box, a fifth seedling cultivation electromagnetic valve, a water pipe, a water pump, a water tank electromagnetic valve, a mixed water tank, a computer control machine, a nutrient solution tank electromagnetic valve, a nutrient solution tank, an inflow electromagnetic valve, a clear water pipe, a fluorescent lamp, a spray head, a seedling cultivation tank, a seedling cultivation box, a supporting platform, a main pipeline, a humidity sensor and a conveying pipe. The atomizer is arranged on the left side of the outer part of the box body. The spraying pipe is arranged above the box body. The water tank electromagnetic valve is connected with a mixed water tank. The mixed water tank and the fifth seedling cultivation electromagnetic valve are connected with the computer control machine. According to the invention, the device is excellent in use effect and the cultivation of different plant seedlings is realized. Nutrient solutions required by different types are added according to different types of plant seedlings. The liquid supply device can effectively mix up nutrient solutions. Therefore, the cultivation environment of plant seedlings in the box body can be effectively controlled.