Preparation method of young mollusks of Hongkong tetraploid oysters

A Hong Kong oyster and tetraploid technology, which is applied in the field of preparation of Hong Kong oyster tetraploid juveniles, can solve the problems that tetraploid juveniles cannot be metamorphosed and research cannot be carried out.

Active Publication Date: 2017-12-22
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

After years of research, scholars have found that by using triploid eggs to fertilize diploid sperm and inhibiting the release of the first polar body of fertilized eggs, tetraploid adults of surviving shellfish can be obt...

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  • Preparation method of young mollusks of Hongkong tetraploid oysters

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Embodiment 1

[0021] a. Prefer triploid eggs: On May 30, 2017, during the breeding season of oysters in Hong Kong, 63 triploid Hong Kong oysters with a shell height of 12-15cm were randomly selected, and the triploids were detected by flow cytometry Individuals, 57 3N individuals were found, and then the triploids were dissected, the gonads were selected with a toothpick, and the sex was identified by microscope examination. 46 female individuals, 10 gonads were not developed, and 1 male individual was found; among 46 female individuals Among them, 7 individuals with relatively full gonads and good egg quality were screened. The eggs were neatly developed, the yolk was darker, and the egg diameter was about 70 μm. The eggs were squeezed out, filtered with a 200-mesh sieve, and placed in a salinity Activated in 15ppt sea water for 30 minutes, then washed the eggs with 400-mesh silk screen, repeated 3 times to remove impurities, after calculation, the number of eggs was between 10 million and ...

Embodiment 2

[0027] a. Prefer triploid eggs: On June 3, 2017, during the oyster breeding season in Hong Kong, 35 triploid Hong Kong oysters with a shell height of 12-15cm were randomly selected, and the triploids were detected by flow cytometry Individuals, 28 3N individuals were found, and then the triploids were dissected, and the gonads were selected with a toothpick, and the sex was identified by microscope examination. 23 female individuals were found, and 5 gonads were not developed; among the 23 female individuals, 5 were screened. Individuals with relatively full gonads and good egg quality, neatly developed eggs, darker yolk, and about 70 μm in diameter, squeezed out the eggs, filtered them with a 200-mesh sieve, and placed the eggs in seawater with a salinity of 20ppt to activate for 60 minutes , and then wash the eggs with a 400-mesh sieve, and repeat twice to remove impurities. After calculation, the number of eggs is between 10 million and 20 million. It is found that the eggs ...

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Abstract

The invention discloses a preparation method of young mollusks of Hongkong tetraploid oysters. The method comprises the following technical steps of selectively choosing triploid eggs, carefully choosing diploid sperms, inhibiting second polar bodies of oosperms, obtaining tetraploid young mollusks, and raising tetraploid progenies. The feature of normal reduction mitosis of some triploid eggs is adopted so as to create a raising method for preparing tetraploid oysters by inhibiting second polar bodies of oosperms (triploid eggs +diploid sperms). The method differs from a method for obtaining tetraploid oysters by inhibiting first polar bodies of oosperms. The preparation method makes a great difference in a traditional preparation mode and helps break the monopolies of the US, France, Korea and other foreign countries on raisins technology of tetraploid oysters. It is the first time to obtain livable young mollusks of Hongkong tetraploid oysters. The method makes the biological method of producing 100% Hongkong triploid oysters feasible, promote the steady development of the industry of Hongkong oysters in the coastal region of southern China and offers the possibility of achieving improved varieties of the industry. The preparation method of young mollusks of Hongkong tetraploid oysters is being advantaged by being implementable, reusable, and applicable.

Description

Technical field: [0001] The invention belongs to the technical field of shellfish genetics and breeding in marine agriculture, and in particular relates to a preparation method of Hong Kong oyster tetraploid juvenile shellfish. Background technique: [0002] A tetraploid is a cell or organism that has four sets of chromosomes, as opposed to a diploid organism that contains only two sets of chromosomes, or a triploid organism that contains three sets of chromosomes. In nature, natural tetraploid mollusks have hardly been reported, and artificially induced tetraploids are mainly concentrated in oysters, scallops, mussels and other species. Usually, the tetraploid production method includes inhibiting the release of the polar body of the fertilized egg, inhibiting the first cleavage, promoting the chromosome doubling of the normal ploidy fertilized egg through cell fusion or gynogenesis, and forming a tetraploid through embryonic development. After years of research, scholars ...

Claims

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Application Information

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IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 张跃环喻子牛秦艳平肖述
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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