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Efficient induction method for autotetraploid loach fish fry

An autotetraploid, loach technology, applied in fish farming, application, climate change adaptation, etc., can solve the problem of low tetraploid occurrence rate and survival rate, hinder the development of new fish species such as loach, and the results are repeatable problems such as poor reproducibility, to achieve the effect of strong repeatability, efficient method and easy operation

Inactive Publication Date: 2017-09-22
HUAZHONG AGRI UNIV
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Problems solved by technology

However, in practice, the researchers found that the induction conditions of autotetraploid fish (such as treatment initiation time, shock temperature and treatment duration, etc.) The emergence rate and survival rate of the body are very low (Chang Zhongjie & Du Qiyan, 2002), and the induction effect is unstable, and the reproducibility of the results is very poor
So far, the artificial induction of autotetraploid has only been successful in rainbow trout and has been applied to production. In loach and other fishes, tetraploid fish that have both sexes and formed groups have not been obtained, which seriously hinders the development of loach and other fishes. development of new species

Method used

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  • Efficient induction method for autotetraploid loach fish fry
  • Efficient induction method for autotetraploid loach fish fry
  • Efficient induction method for autotetraploid loach fish fry

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Embodiment 1

[0025] A method for efficiently inducing loach autotetraploid fry, comprising the steps of:

[0026] (1) Diploid male and female loach with well-developed selective glands and strong physique were used as parents, and LHRH-A 2 +DOM mixed injection method was used to artificially induce labor, and after the effect time was reached, artificial insemination was carried out by semi-dry method. After mixing the fertilized eggs evenly, spread them on a petri dish (not less than 100 fertilized eggs in each petri dish), and incubate them in aerated water at 25°C for 28 minutes.

[0027] (2) Divide fertilized eggs into 4 groups:

[0028] The first group: the control group (3 repetitions), without any treatment, the fertilized eggs were directly placed in 25°C aerated water for hatching;

[0029] The second group: heat shock treatment only, 1min, 2min and 3min respectively in 40.5°C water bath (3 repetitions for each time gradient);

[0030] The third group: cold shock treatment only...

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Abstract

The invention belongs to the technical field of fish polyploid breeding, and specifically discloses an efficient induction method for autotetraploid loach fish fry. The method comprises the specific steps that eggs and sperms of wild diploid loaches are enabled to conduct insemination in the conventional way, fertilized eggs are kept in a water bath at 40.5+ / -0.5 DEG C for heat shocking treatment for 2-3 min, and transferred quickly in cold water of 1.5+ / -0.5 DEG C for cold shocking treatment for 30-60 min, and after the temperature of the fertilized eggs turns normal, the fertilized eggs are continued to be incubated until the emergence of the fish fry. By combining heat shocking with cold shocking dynamically, the efficient induction method for autotetraploid loach fish fry is established. According to the efficient induction method for autotetraploid loach fish fry, the yield rate of the autotetraploid loach fish fry reaches 10.4+ / -0.3%, far higher than that of a single heat shocking method or a single cold shocking method in the traditional way. The efficient induction method for autotetraploid loach fish fry has the advantages of being simple and easy to operate, good in repeatablity, high in induction efficiency and the like, and provides technical support for polyploid loach breeding and related genetic studies to polyploidization.

Description

technical field [0001] The invention belongs to the technical field of cell engineering breeding, and specifically discloses a method for efficiently inducing autotetraploid fry of loach. Background technique [0002] Autotetraploid refers to individuals whose somatic cells contain four sets of chromosome sets from the same source, usually resulting from the doubling of chromosomes in diploid organisms. The artificial induction of fish autotetraploid is of great significance in aquatic animal breeding research. On the one hand, fertile artificial tetraploid fish can be mated with common diploid fish to obtain a large number of triplets that can be used for aquaculture production. It solves the problems of artificially induced triploid production scale limitations, unstable induction efficiency and high embryonic mortality, and is the best method for large-scale production of triploid fish; Source tetraploid, because its origin ancestor is known exactly, and, compared with d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 周小云冯兵
Owner HUAZHONG AGRI UNIV
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