Megalobrama amblycephala breeding method

A technology of bream and heterogeneity, applied in fish farming, application, climate change adaptation, etc., can solve the problems of reducing the number of effective populations and increasing the probability of inbreeding

Inactive Publication Date: 2019-03-08
SHANGHAI OCEAN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, with the development of artificial breeding, there may be an accumulation of factors ...

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  • Megalobrama amblycephala breeding method
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[0052] In order to better explain the technical solutions of the present invention, the embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. The following examples are used to further illustrate the present invention, but should not be construed as fixing or limiting the present invention. Unless otherwise specified, the technical features used in the embodiments may be replaced with other technical features known in the art that have equivalent or similar functions or effects without departing from the inventive concept.

[0053] 1 Materials and methods

[0054] 1.1 Materials

[0055] The female fish used in the experiment was 3-year-old bream tolerant to hypoxia F 4 generation, for literature [5] Public F 3 F 4 For the second generation, select individuals with a body weight of about 1000g, good body shape, sexual maturity and good development, and use a one-time injection at the base of the pectoral fins,...

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Abstract

The invention discloses a megalobrama amblycephala breeding method. The breeding method comprises the following steps of a, inactivation of heterogeneous male fish sperms, wherein the heterogeneous male fish sperms which are obviously weak in activity, not dead and inactivated are prepared; b, activation of megalobrama amblycephala eggs, wherein the inactivated heterogeneous male fish sperms and the megalobrama amblycephala eggs are subjected to fertilization so as to obtain activated megalobrama amblycephala eggs; c, diploid induced activation of the megalobrama amblycephala eggs, wherein (i)or (ii) is selected; according to (i) cold shock induction, the activated megalobrama amblycephala eggs start to be subjected to cold shock treatment for 10-30 min in the environment at the temperature of 0-4 DEG C 0-6 min after activation; according to (ii) hydrostatic pressure induction, the activated megalobrama amblycephala eggs start to be subjected to hydrostatic pressure shock treatment for 1.5-5 min at the pressure of 40-50 MPa 0-6 min after activation; d, the diploid induced megalobrama amblycephala eggs obtained in step c are incubated so as to obtain megalobrama amblycephala with gynogenesis.

Description

technical field [0001] The invention belongs to the field of aquatic products, and relates to a breeding method of bream, in particular to a method for inducing gynogenesis of a new strain of bream which is resistant to hypoxia with heterologous sperm. Background technique [0002] Megalobrama amblycephala, also known as Wuchang fish, is a herbivorous economic fish with the characteristics of delicious meat, high meat content, wide feeding habits, fast growth, and strong disease resistance. [1] . According to the latest statistics from the Food and Agriculture Organization of the United Nations (FAO), the domestic production in 2016 was about 830,000 tons, making it one of the main freshwater aquaculture targets in China. However, in recent years, during the breeding process, bream has shown a trend of slowing down in growth rate, precocious puberty, thinning body shape, and declining meat quality. mixed threat [2] , its improved variety breeding work has become an urgent...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 邹曙明王东东郑国栋吴成宾陈杰徐文迪
Owner SHANGHAI OCEAN UNIV
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