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Method for inducing gynogenesis of megalobrama amblycephala by koi sperm and application of method

A technique for gynogenesis and gynogenesis of bream, which is applied in the field of koi sperm-induced gynogenesis of bream, can solve problems such as the inability to improve the germplasm resources of bream, the poor hypoxia tolerance of red bream, The problem of irritable temperament of the red trout with upturned mouth can achieve the effect of good sperm activity, large amount of semen and strong resistance to stress.

Pending Publication Date: 2019-08-02
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The applicant's laboratory previously stimulated the gynogenesis of the bream with the semen of the red bream, and obtained the gynogenetic bream. However, the hybrid offspring of the bream and the red bream were produced from the gynogenetic offspring, and it was difficult to obtain gynogenetic progenies, in addition, the bream itself is not resistant to hypoxia, and the ability of red bream to tolerate low oxygen is relatively poor, so it cannot improve the germplasm resources of bream; and the bream Red bream has a violent temperament, and after induced labor, most red bream are difficult to survive, causing considerable economic losses. These problems limit the promotion and application of gynogenesis technology in roundhead bream

Method used

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  • Method for inducing gynogenesis of megalobrama amblycephala by koi sperm and application of method
  • Method for inducing gynogenesis of megalobrama amblycephala by koi sperm and application of method
  • Method for inducing gynogenesis of megalobrama amblycephala by koi sperm and application of method

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Effect test

Embodiment 1

[0041] A method for inducing gynogenesis of bream by koi sperm of the present invention comprises the following steps:

[0042] (1) Selection and cultivation of broodstock: 3 months before breeding, select male koi and female bream with beautiful body, no disease and injury, and obvious sexual maturity characteristics as broodstock for intensive rearing in separate ponds. During this period, fine bait is fed, and the water quality remains good; one month before the breeding season, water stimulation is performed every two days to promote the maturation of the gonads of broodstock;

[0043] (2) Artificial oxytocin: From the beginning of May to the end of May of the current year, when the water temperature reaches above 20°C, inject a mixed oxytocic agent of lutein-releasing hormone analogue (LRH-A) and chorionic gonadotropin (HCG) to the parent fish To induce labor, the dosage of LRH-A is 10ug / kg, and the dosage of HCG is 500IU / kg; both parent fish and parent fish are injected ...

Embodiment 2

[0067] A method for inducing gynogenesis of bream by koi sperm of the present invention comprises the following steps:

[0068] (1) Selection and cultivation of broodstock: 4 months before breeding, select male koi and female bream with beautiful body, no disease and injury, and obvious sexual maturity characteristics as broodstock for intensive rearing in separate ponds. During this period, fine bait is fed, and the water quality remains good; one month before the breeding season, water stimulation is performed every two days to promote the maturation of the gonads of broodstock;

[0069] (2) Artificial oxytocin: From the beginning of May to the end of May of the current year, when the water temperature reaches above 20°C, inject a mixed oxytocic agent of lutein-releasing hormone analogue (LRH-A) and chorionic gonadotropin (HCG) to the parent fish To induce labor, the dosage of LRH-A is 10ug / kg, and the dosage of HCG is 500IU / kg; both parent fish and parent fish are injected ...

Embodiment 3

[0077] A method for inducing gynogenesis of bream by koi sperm of the present invention comprises the following steps:

[0078] (1) Selection and cultivation of broodstock: 3 months before breeding, select male koi and female bream with beautiful body, no disease and injury, and obvious sexual maturity characteristics as broodstock for intensive rearing in separate ponds. During this period, fine bait is fed, and the water quality remains good; one month before the breeding season, water stimulation is performed every two days to promote the maturation of the gonads of broodstock;

[0079] (2) Artificial oxytocin: From the beginning of May to the end of May of the current year, when the water temperature reaches above 20°C, inject a mixed oxytocic agent of lutein-releasing hormone analogue (LRH-A) and chorionic gonadotropin (HCG) to the parent fish To induce labor, the dosage of LRH-A is 10ug / kg, and the dosage of HCG is 500IU / kg; both parent fish and parent fish are injected ...

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Abstract

The invention discloses a method for inducing the gynogenesis of megalobrama amblycephala by koi sperm, which roughly comprises the following steps: obtaining a mature ovum and the koi semen of the megalobrama amblycephala by artificial spawning, inactivating the koi semen with a 18-20W ultraviolet lamp for 26 to 30 min, then mixing with the ovum of the megalobrama amblycephala, activating the eggin normal temperature water for 2 to 3 min, then performing cold treatment in cold water at 4 to 6 DEG C for 20 to 22 min, performing running water incubation in normal temperature water, and continuing cultivation to obtain the megalobrama amblycephala in gynogenesis. The koi sperm is adopted to induce the gynogenesis of the megalobrama amblycephala, a megalobrama amblycephala offspring in gynogenesis is obtained by successful breeding, and the growth rate, the meat quality, the stress resistance and the hypoxia tolerance are obviously stronger than those of common megalobrama amblycephala during a breeding process. The method has obvious advantages in breeding, which not only provides a good foundation for improving germplasm resources of the megalobrama amblycephala, but also has greatsignificance in genetic breeding and biological evolution.

Description

technical field [0001] The invention belongs to the field of fish germplasm resources improvement, and in particular relates to a method for inducing gynogenesis of bream by koi sperm and an application thereof. Background technique [0002] Megalobrama amblycephala (Megalobrama amblycephala) belongs to the family Cyprinidae, the subfamily of Cyprinidae, and the genus Bream. It is loved by many farmers because of its beautiful body, delicious meat, and herbivorous nature. It is the main economic fish in freshwater fish polyculture. In my country Plays a very important role in aquaculture. However, in recent years, the growth rate of the bream has slowed down, and the quality of the meat has deteriorated. In addition, the bream is extremely intolerant to low oxygen, and the sweltering summer can easily lead to pond flooding, causing huge economic losses. Therefore, it is imminent to improve the germplasm resources of bream. [0003] The applicant's laboratory attaches great ...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 刘少军龚丁斌王跃群罗凯坤陶敏覃钦博张纯胡方舟王余德王石汤陈宸吴昌
Owner HUNAN NORMAL UNIVERSITY
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