Method for inducing gynogenesis of megalobrama amblycephala by koi sperm and application of method

A technique for gynogenesis and gynogenesis of bream, which is applied in the field of koi sperm-induced gynogenesis of bream, can solve problems such as the inability to improve the germplasm resources of bream, the poor hypoxia tolerance of red bream, The problem of irritable temperament of the red trout with upturned mouth can achieve the effect of good sperm activity, large amount of semen and strong resistance to stress.
CN110074022APending Publication Date: 2019-08-02HUNAN NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUNAN NORMAL UNIVERSITY
Publication Date
2019-08-02

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Abstract

The invention discloses a method for inducing the gynogenesis of megalobrama amblycephala by koi sperm, which roughly comprises the following steps: obtaining a mature ovum and the koi semen of the megalobrama amblycephala by artificial spawning, inactivating the koi semen with a 18-20W ultraviolet lamp for 26 to 30 min, then mixing with the ovum of the megalobrama amblycephala, activating the eggin normal temperature water for 2 to 3 min, then performing cold treatment in cold water at 4 to 6 DEG C for 20 to 22 min, performing running water incubation in normal temperature water, and continuing cultivation to obtain the megalobrama amblycephala in gynogenesis. The koi sperm is adopted to induce the gynogenesis of the megalobrama amblycephala, a megalobrama amblycephala offspring in gynogenesis is obtained by successful breeding, and the growth rate, the meat quality, the stress resistance and the hypoxia tolerance are obviously stronger than those of common megalobrama amblycephala during a breeding process. The method has obvious advantages in breeding, which not only provides a good foundation for improving germplasm resources of the megalobrama amblycephala, but also has greatsignificance in genetic breeding and biological evolution.
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Description

technical field

[0001] The invention belongs to the field of fish germplasm resources improvement, and in particular relates to a method for inducing gynogenesis of bream by koi sperm and an application thereof. Background technique

[0002] Megalobrama amblycephala (Megalobrama amblycephala) belongs to the family Cyprinidae, the subfamily of Cyprinidae, and the genus Bream. It is loved by many farmers because of its beautiful body, delicious meat, and herbivorous nature. It is the main economic fish in freshwater fish polyculture. In my country Plays a very important role in aquaculture. However, in recent years, the growth rate of the bream has slowed down, and the quality of the meat has deteriorated. In addition, the bream is extremely intolerant to low oxygen, and the sweltering summer can easily lead to pond flooding, causing huge economic losses. Therefore, it is imminent to improve the germplasm resources of bream.

[0003] The applicant's laboratory attaches great ...

Claims

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