A kind of rapid propagation method of short-distance grove japonica

A kind of short-distance grove, fast technology, applied in the field of plant tissue culture, can solve the problems of low coefficient, difficult to meet the requirements of protection and development, difficult to multiply and so on, achieve simple preparation, solve the problem of resource protection, transplant survival rate high effect

Active Publication Date: 2017-03-08
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the plants of the genus Grogonia still adopt the traditional orchid ramet technique, that is, the steps of raising seedlings, washing roots, branching, and disinfecting, etc., the multiplication will be difficult and the coefficient will be low, which will form a barrier for the effective propagation of the short-distance Grogula , it is difficult to meet the protection and development requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Seed germination: Use a healthy, disease-free plant as the mother plant, and artificially pollinate the short-distance grooved plant according to the routine, that is, in May to June each year, that is, the flowering season of short-distance grooved plant. 5-7 days after flowering, select healthy and disease-free plants for pollination and self-pollination at 10-12 a.m. on a sunny and rain-free day. The plants are not sprayed with water one day before and one day after selfing; 120 days after pollination, The mature, disease-free, and unruptured intact fruit of the capsule is routinely sterilized, that is, the capsule of the short-distance groswellia is rinsed with running water for 30 minutes, and the surface of the capsule is wiped with 70% alcohol on the super bacteria workbench, and then sequentially Wash with sterile water 3 times, sterilize with 0.1% mercury liters for 10 minutes, wash 3 times with sterile water, then soak with 10% sodium hypochlorite for 10 min...

Embodiment 2

[0026] (1) Seed germination: Use a healthy, disease-free plant as the mother plant, and artificially pollinate the short-distance grooved plant according to the routine, that is, in May to June each year, that is, the flowering season of short-distance grooved plant. 5-7 days after flowering, select healthy and disease-free plants for pollination and self-pollination at 10-12 a.m. on a sunny and rain-free day. The plants are not sprayed with water one day before and one day after selfing; 120 days after pollination, The mature, disease-free, and unruptured intact fruit of the capsule is routinely sterilized, that is, the capsule of the short-distance groswellia is rinsed with running water for 30 minutes, and the surface of the capsule is wiped with 70% alcohol on the super bacteria workbench, and then sequentially Wash with sterile water 3 times, sterilize with 0.1% mercury liters for 10 minutes, wash 3 times with sterile water, then soak with 10% sodium hypochlorite for 10 min...

Embodiment 3

[0034] (1) Seed germination: Use a healthy, disease-free plant as the mother plant, and artificially pollinate the short-distance grooved plant according to the routine, that is, in May to June each year, that is, the flowering season of short-distance grooved plant. 5-7 days after flowering, select healthy and disease-free plants for pollination and self-pollination at 10-12 a.m. on a sunny and rain-free day. The plants are not sprayed with water one day before and one day after selfing; 120 days after pollination, The mature, disease-free, and unruptured intact fruit of the capsule is routinely sterilized, that is, the capsule of the short-distance groswellia is rinsed with running water for 30 minutes, and the surface of the capsule is wiped with 70% alcohol on the super bacteria workbench, and then sequentially Wash with sterile water 3 times, sterilize with 0.1% mercury liters for 10 minutes, wash 3 times with sterile water, then soak with 10% sodium hypochlorite for 10 min...

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PUM

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Abstract

The present invention discloses a method for rapid propagation of Gosquillum spp., through the steps of seed germination, induction of protocorms, differentiation culture, strong seedling cultivation, rooting culture, hardening and transplanting of seedlings, etc., to complete the growth of Gospela spp. Rapid reproduction. Compared with the traditional branching method, the present invention can increase the multiplication coefficient of G. japonica by more than 5 times, the seedling rate can reach 95%, and the transplanting survival rate can reach more than 85%. Fast speed, strong plants, low browning rate, high transplanting survival rate, simple medium preparation, low cost, etc., can adapt to large-scale industrial production.

Description

Technical field [0001] The invention relates to a method for the propagation of rare and endangered orchid plants, in particular to a method for the rapid propagation of short-distance trough hyacinth, which belongs to the technical field of plant tissue culture. Background technique [0002] Short grooved tongue ( Holcoglossumflavescens(Schltr.)Z.H.Tsi ) Is Orchid ( Orchidaceae ) Groophyllum (Holcoglossum )plant. Produced in northern Fujian (Mount Wuyi, Jianyang), southwestern Hubei (Lichuan), southwestern Sichuan (Panzhihua), and northern Yunnan (Binchuan, Yongsheng). Born on the trunk of an evergreen broad-leaved forest at an altitude of 1200 to 2000 meters. The short-span grooved tongue orchid is a treasure in the orchid family, its shape is elegant, the flower color is gorgeous, and it exudes fragrance. It can be placed in shallow pots with fern roots and other attached plants, or hung. There are a total of 12 species of the genus Cercophyllum, distributed from Southea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李涵曹桦李慧敏蒋亚莲阮继伟黎霞赵培飞杨维杨春梅
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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