Method for artificially inducing sexual glands of Anguilla marmorata to develop maturely

A mature and artificially induced technology, which is applied in the field of eel artificial reproduction technology and hormone-induced flower eel gonad development and maturation, can solve the problem that the gonad cannot mature naturally

Inactive Publication Date: 2013-10-23
HAINAN UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for artificially inducing the maturation of the gonads of the flower eel, so as to solve the problem that the gonads of the flower eel cannot mature naturally under artificial feeding conditions

Method used

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  • Method for artificially inducing sexual glands of Anguilla marmorata to develop maturely
  • Method for artificially inducing sexual glands of Anguilla marmorata to develop maturely
  • Method for artificially inducing sexual glands of Anguilla marmorata to develop maturely

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Embodiment Construction

[0021] The following implementation method is a specific application example of the present invention, but not limited to this example.

[0022] (1) Put the collected artificial pond-raised eels into glass jars at a density of 1 fish / tank for domestication, adopt an indoor circulating aquaculture system, keep away from light 24 hours a day, and control the water temperature at 22-26°C. The domestication time is 1 month. During the domestication period, live bait and eel powder are mixed and fed, 3 times a day, and the leftover feed is cleaned up after 3 hours of feeding each time.

[0023] (2) It takes a certain amount of time for broodstock to go through the whole process of osmotic pressure and physiological adjustment from fresh water to semi-sea water. Female flower eels were not fed with bait during salinity adjustment. From the second day after the feeding was stopped, the daily salinity was increased by 2-3 until the salinity increased to 14-17, which lasted for 7-9 da...

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Abstract

The invention discloses a method for artificially inducing sexual glands of Anguilla marmorata to develop maturely. The method comprises the following steps of: accelerating the maturation of male Anguilla marmorata by intramuscularly injecting human chorionic gonadotropin (HCG), wherein the dosage is 500U / kg based on the body weight of the male Anguilla marmorata, the HCG is injected once every week, and the injection time is 6 weeks; and accelerating the maturation of female Anguilla marmorata by intramuscularly injecting luteinizing hormone releasing hormone A2 (LHRH-A2) and carp pituitaryextract (CPE) homogenate, the dosage of the LHRH-A2 is 250mu g / kg based on the body weight of the female Anguilla marmorata, the dosage of CPE is 4 grains / kg based on the body weight of the female Anguilla marmorata, the LHRH-A2 and the CPE are injected once every 7 to 9 days, and the injection times are 15 to 18. By the scheme, sexual glands of parent fish are developed maturely, abdomens are remarkably protuberant, and the sexual glands are remarkably in a spongy state when convex parts of the abdomens are touched by hands; the female Anguilla marmorata can release mature eggs; and milky white semens can be collected by extruding the abdomens of the male Anguilla marmorata. The method provides a preliminary technical assurance for further researching the completely artificial propagation of the Anguilla marmorata.

Description

technical field [0001] The invention belongs to the field of aquaculture, and relates to an artificial propagation technology of eel, in particular to a method for hormone-induced gonad development and maturation of eel. Background technique [0002] Anguilla marmorata (Anguilla marmorata), commonly known as bass eel, flower eel, snow eel, king eel, stream eel, etc., belongs to Osteichthyes, Anguilliformes, Anguillidae, genus Anguilla ( Anguilla) is the most widely distributed species in the genus Eel. Its distribution ranges from the central islands of the Pacific Ocean in the east to Madagascar in the Indian Ocean in the west, southern Australia in the south, and North Korea and southern Japan in the north. In my country, it is mainly found in the rivers and seas of Hainan, Guangdong, Fujian, Taiwan and other provinces. The flower eel is a kind of migratory fish that reproduces in the sea. It grows in freshwater rivers and lakes. After sexual maturity, it migrates to the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 尹绍武林浩然齐鑫周雯伊齐兴柱骆剑
Owner HAINAN UNIV
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