Penaeus vannamei boone half-sib family construction method
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A construction method and semi-sibling technology, applied in the field of aquaculture, can solve problems such as shedding, and achieve the effect of avoiding inbreeding depression
Inactive Publication Date: 2015-11-11
GUANGDONG OCEAN UNIVERSITY
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[0005] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a method for constructing half-sib families of Penaeus vannamei, which has a high fertilization rate, does not need artificial sperm pod transplantation, does not cause harm to female shrimp, and can simultaneously establish multiple In the half-sib family, the sperm are artificially released i...
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Embodiment 1
[0021] Embodiment 1 adopts the following steps to realize the present invention:
[0022] (1) Selection of mature broodstock:
[0023] a. In the broodstock breeding pond, the female and male shrimps were intensively cultivated respectively, and the female and male shrimps whose gonads had matured were used as alternative broodstock; , 28±0.5℃, salinity is 31±1, dissolved oxygen is controlled at 5mg.L -1 ,
[0024] b. On the day when the family line is established, select the broodstock in the broodstock breeding pond in step a according to the following conditions: select a strong body, sound appendages, transparent body color, full stomach and intestines, smooth body surface, no attachments, and the size is 35-50 gram / tail, body length 15-18cm, broodstock shrimps that do not carry specific pathogens after regular sampling inspection; among them, the selection criteria of female shrimps are: the color of gonads of female shrimps is red when observed from the surface, the l...
Embodiment 2
[0033] Embodiment 2 adopts the following steps to realize the present invention:
[0034] (1) Selection of mature broodstock: On the day when the family line was established, broodstock selection was carried out in the broodstock cultivation pool of Example 1 according to the following conditions: select strong body shape, sound appendages, transparent body color, full stomach and intestines, and smooth body surface , without attachments, the specification is 35-50 grams per tail, the body length is 15-18cm, and broodstock shrimps that do not carry specific pathogens have been regularly sampled and inspected; among them, the selection criteria for female shrimps are: the color of the gonads of female shrimps is red when observed from the surface, The ovaries of each body segment are clearly divided into lobes, the gonads have large protrusions on the first and second abdominal segments, and the eggs are full. According to the development of the gonads, it is judged that the fem...
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Abstract
The invention discloses a penaeus vannamei boone half-sib family construction method. The method comprises the steps: selecting mature parent shrimps, marking an individual parent shrimp, setting a mating scheme, acquiring spermatophore, activating sperms, laying eggs by female shrimps, collecting eggs, performing artificial insemination, hatching germ cells, collecting nauplius, and the like. Compared with the traditional artificial spermatophore transplanting method, the method disclosed by the invention has the advantages that no harm on the female shrimp is produced, and a plurality of half-sib families can be simultaneously established; the sperms are artificially released into an egg laying water body, so that the weakness of no mating or separation of the mated female shrimp spermatophore caused by the natural mating can be avoided; on the premise of a set inbreeding coefficient, the mating scheme is artificially designed, so that the inbreeding depression of the constructed family can be effectively avoided, and the method is a high-efficient penaeus vannamei boone half-sib family construction method.
Description
technical field [0001] The invention relates to a line selection method of Penaeus vannamei, in particular to a method for constructing half-sib lines of Penaeus vannamei, belonging to the technical field of aquaculture. Background technique [0002] The Penaeus vannamei industry is a pillar industry of my country's fishery economy, and there is a huge demand for Penaeus vannamei seedlings in my country every year. However, the high-quality broodstock of Penaeus vannamei in my country still mainly depends on imports, which seriously restricts the development of my country's Penaeus vannamei industry. Therefore, strengthening the breeding of fine varieties of Penaeus vannamei is the key to realizing the sustainable development of my country's vannamei industry. [0003] In recent years, the Hawaii Oceanographic Research Institute in the United States has bred fast-growing strains and taura-resistant Penaeus vannamei strains through family selection combined with comprehensiv...
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Application Information
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