50 results about "Unfertilized Eggs" patented technology

The invention relates to a method for cultivating hybridized and backcrossed marine product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten purpuratus and Argopecten irradians, and synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten irradians; inducing the hybridized first filial generation individuals to spawn by using a conventional shade drying and temperature stimulation method so as to obtain unfertilized eggs; simultaneously, stimulating the Argopecten irradians to spawn and discharge seminal fluid, and filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten irradians; and fertilizing the hybridized first filial generation eggs with the seminal fluid of the Argopecten irradians, cultivating according to a conventional method after hatching germ cells, and thus obtaining the hybridized and backcrossed marine product fries which are above 1.5 cm in shell height and used for commercial cultivation. The hybridized and backcrossed marine product fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed marine product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed marine product fries can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

A hatching egg inspection apparatus includes a loader unit, an inspection unit for determining the viability or the like as to whether a hatching egg is an unfertilized egg or a growth-stopping egg, a rejection unit for rejecting a hatching egg determined to be an unfertilized egg or the like, and an unloader unit for sending a hatching egg toward the next step. The inspection unit is provided with a light emitting unit for emitting prescribed light toward the hatching egg, a cap attachment unit through which the light having been emitted to the hatching egg and having transmitted through the hatching egg passes, and a light receiving unit for receiving the light that has passed through the cap attachment unit. The cap attachment unit includes a cylindrical cap coming into contact with the hatching egg, and a plate to which the cap is attached.

The invention provides a wild duck breeding egg hatching method. The wild duck breeding egg hatching method comprises the steps of secondary sterilization of breeding eggs, control of hatching temperature and humidity of the breeding eggs, control of the oxygen content in an hatching chamber, egg cooling, egg turning, egg lighting, egg traying, hatching and the like. The breeding eggs are soaked by adopting a vitamin C solution, the permeability of egg shells is improved to some degree, the egg shells are channels for gas exchange and moisture evaporation in the embryonic development process and can have a certain influence on the permeability of the egg shells and the gas exchange and moisture evaporation of embryos and accordingly affect the water loss rate of the eggs and improve a hatching affecting effect, dead eggs, unfertilized eggs and dead embryos are removed in time through multiple times of egg lighting, the changing temperature, humidity and oxygen content are accurately controlled at all hatching stages, the hatching rate is high, different specifications of grouped breeding eggs are consistent in hatching time, convenience is brought to intensive work of workers, and the wild duck breeding egg hatching method can meet the demand for large-scale industrial raising.

The invention discloses a method for increasing the artificial incubation rate of tilapia mossambica. The method comprises the following steps of rinsing fertilized eggs with physiological saline; soaking the rinsed fertilized eggs with levothyrocine T4 solution; incubating the soaked fertilized eggs in running water in which the levothyrocine T4 solution with the concentration of 0.05ppm is added. The method disclosed by the invention has the beneficial effects that unfertilized eggs and eggs with a poor fertilizing effect can be rejected by rinsing, soaking and incubating; meanwhile, the fertilized eggs can absorb levothyrocine T4 from the outside environment, so that the requirement on the levothyrocine T4 during incubation can be ensured, and the incubation rate of the fertilized eggs can be increased. During artificial incubation of the tilapia mossambica, the artificial incubation rate and the fry survival rate are increased, and the yield of fries is increased.

The invention relates to a cynoglossus semilaevis triploid fry mass inducing method which comprises an inducing method and an authenticating method of a triploid fry. The inducing method comprises the steps of collection and insemination of an unfertilized egg, confirmation of triploid induction starting time and the confirmation of hydrostatic shock pressure and processing time; and the authenticating method comprises the steps of authentication with a ploidy tester and the analyzing authentication of chromosome. The method for producing cynoglossus semilaevis triploid fries massively by adopting hydrostatic induction is established at first; the proper start time and the hydrostatic shock condition of triploid induction are screened; and the triploid fry mass inducing method is established. The inducing rate of the triploid fry obtained by the method disclosed by the invention is as high as more than 95 percent. The method established by the invention has the characteristics of advancement, high efficiency, accuracy and reliability, has important application value in cynoglossus semilaevis triploid induction and sterile seedling production and has broad popularization and application prospect in fish cultivation and breeding.

The invention relates to the field of hepialus lagii egg screening technologies, in particular to a hepialus lagii egg screening device. The hepialus lagii egg screening device comprises a box and a scattering device located above the box. A rotatable adsorption barrel with static electricity is arranged in the box. A hole is formed in the top end of the box, a discharging channel of the scattering device stretches into the box through the hole, and a discharging port of the scattering device is located above the adsorption barrel. An egg receiving case with at least two boxes is arranged at the bottom of the box, and cord fabric is arranged on the inner wall of the box and covers the egg receiving case. According to different adsorption forces between the adsorption barrel with static electricity and impurities, broken eggs, unfertilized eggs, non-full fertilized eggs and full fertilized eggs, different substances will fall to different positions of the cord fabric of the egg receiving case under the rotating effect of the adsorption barrel and the gravity of the substances, and therefore the eggs can be screened.

An object of the invention is to effectively prepare a fish embryo with a correct chromosomal ploidy by nuclear transplantation in which an exogenous fish nucleus is transplanted in a cytoplasmic recipient. For this object, the invention comprises a step of preparing a fish embryo by transplanting a fish cell nucleus to an unfertilized egg. The step of preparing a fish embryo comprises a step of imposing physical and/or chemical stress to the unfertilized egg after activation. By imposing such stress, the stage of haplosis in a female nucleus which happens at the early stage of a series of developmental steps occurring in an unfertilized egg is suppressed and the correct ploidy of an obtained embryo is at least secured.

The invention provides an application method for a hatching egg sorting tool. the method comprises the following steps of A, turning on the LED tankoscope of a lower clamp plate, opening a finger ring to spread out an upper clamp plate and the lower clamp plate at the front end of a clamp arm, and conducting the egg clamping operation; B, closing the finger ring, supporting two clamp arms by positioning columns at the tail end of the clamp arms, avoiding the excessive closing of the finger ring, and avoiding the breakage of an egg between the upper clamp plate and the lower clamp plate, wherein the egg is located between the upper and lower clamp plates at the moment; C, through the perspective window of the upper clamp plate, observing the e internal condition of the egg, and judging the egg is fertilized on the condition that black points occur near the internal yolk portion of the egg under the mapping effect of the LED tankoscope; D, selecting the LED tankoscope to be red or blue. The front end of the LED tankoscope is additionally provided with a scattering cover. The method disclosed by the invention is simple and convenient to use. Based on the method, whether an egg is fertilized or not can be effectively distinguished. Therefore, unfertilized eggs and fertilized eggs are conveniently classified.

The invention relates to a quality detection method for an unfertilized egg of turbot. When the RNA (Ribose Nucleic Acid) concentration of the unfertilized egg of the turbot is 780+/-0.001mu g/egg and the ratio of Pr to RNA to DNA (Deoxyribose Nucleic Acid) is (227-250) to (12-13.5) to (1-1.2), the fertility rate and the hatchability are highest and the quality of the egg is optimal at the same time. According to the quality detection method disclosed by the invention, the quality of turbot eggs can be quickly and effectively detected, thereby quickly determining whether the eggs to be researched meet the requirement demand and increasing the production efficiency; and meanwhile, the quality evaluation system of teleostean eggs is further completed, and an effective platform and an reference are provided for the quality evaluation for researching other cultivated seawater fish eggs.

Provided are a method for preparing a mammalian ovum or embryo in which zona pellucida has been thinned or eliminated, and a method for fertilization using the mammalian ovum prepared by the aforementioned method. The method for thinning or eliminating zona pellucida of a mammalian ovum or embryo involves treating or culturing the mammalian ovum or embryo (such as an unfertilized ovum, a fertilized ovum, or an embryo in the early stages of development) in a culture medium containing a reducing agent having SH groups (such as reduced glutathione or DTT). The resulting mammalian ovum or embryo (such as an unfertilized ovum, a fertilized ovum, or an embryo in the early stages of development) in which zona pellucida has been thinned or eliminated is capable of realizing an improved fertilization rate and development rate when used for in vitro fertilization, transplantation of a fertilized ovum, or preparation of an embryo in the early stages of development used in the production of a genetically modified animal.

The invention discloses a method for inducing silkworm meiosis type parthenogernesis by liquid refrigerants, belonging to the field of insect genetic breeding technology. The method disclosed by the invention comprises the following steps: (1), collecting and cleaning unfertilized eggs; (2), classifying and preparing the liquid refrigerants; (3), performing cold shock on the unfertilized eggs; and (4), protecting the unfertilized eggs subjected to cold shock and hastening the hatching of the silkworm eggs. According to the invention, a silkworm meiosis type parthenogernesis inducing test achieves success by taking the liquid refrigerants as a medium and Chinese silkworms and Chinese-Japanese crossbreeding silkworms as materials, male silkworm individuals in a genotype homozygous type are obtained, and a new approach is provided for obtaining novel homozygous and true hybrid genetic germplasm resources of Chinese silkworms, and further developing research and utilization of gene expression, excellent character formation, heterosis mechanisms and the like.

The invention discloses a method of Platichthys stellatus Pallas reduction gynogenesis with induction of pressure shock, comprising the steps: (1) establishing sperm cold storage for perches, (2) inactivating sperm genetic substances, (3) collecting and impregnating unfertilized eggs of Platichthys stellatus Pallas, (4) conducting induction of pressure shock towards fertilized eggs, and (5) cultivating gynogenetic eggs.The method of Platichthys stellatus Pallas reduction gynogenesis with induction of pressure shock has the advantages of improving the fertility rate and developmental rate of Platichthys stellatus Pallas's gynogenesis and establishing gynogenetic groups of Platichthys stellatus Pallas.

The invention discloses an immunization method of chicken embryos, comprising the following immunization steps of (1) sealing, steaming and disinfecting the immunization space with potassium permanganate and formalin in each cubic meter; (2) disinfecting the chicken embryo air-chamber with alcohol of which the concentration is 75%; (3) candling the chicken embryos of which the age is 18-19 days to screen the dead eggs or the unfertilized eggs; (4) diluting the immunization vaccine with 200-220 milliliters of diluent in each 4000 dose; (5) immunizing the single strain with the immunization chicken embryo with the diluted vaccine, wherein the immunization part is the air-chamber part of the chicken embryo; and (6) sealing, which is to seal the immunization needle holes of the chicken embryo eggs which are immunized. In the invention, the immunization space and the immunization parts are firstly disinfected, so the infection possibility is reduced, and the temperature and the humidity of the immunization space are stabilized; and because of the immunization sealing, the immunization effect is ensured, the stress death of the chicken embryos is prevented, and the hatch loss is reduced.

The invention discloses a control method of sexuality of Gekko japonicus juveniles. The control method comprises the following steps of 1) going through the appearances and the colors of collected Gekko japonicus ova, selecting the ova, which are provided with a complete fragile egg shell and is ivory and faint pink, and removing unfertilized ova and the ova with the formed embryos; 2) placing the Gekko japonicus ova, which conform to the requirements, into a plastic incubation box provided with a cover, laying damp vermiculites with the thickness of 1-1.5 cm at the bottom of the box, and burying the half of the ova into the damp vermiculites; 3) setting the temperature of the plastic incubation box within the scope of 28-32 DEG C, and inoculating in a constant temperature room for 40-56 days. According to the control method, the Gekko japonicus juveniles with the required sexuality are obtained through controlling the inoculation temperature and dropping a letrozole anhydrous alcohol solution.

Methods are provided for delivery of at least one agent into egg(s) from an egg-producing aquatic animal including contacting fertilized or unfertilized egg(s) from said egg-producing aquatic animal with the at least one agent in the presence of a guanidine-containing compound capable of enhancing the permeability of the chorion of the egg(s). Methods are provided for the drug screening and compound toxicity assays. Methods are also provided for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of agents that lead to the failure of fertile gonadal development. Agents may be delivered to the eggs directly before the fertilization or post fertilization by contacting eggs in an immersion medium including the agent of interest.

A recoverable intra-uterine device comprises a housing (11) containing one or several elements selected from a group containing an embryo, male and/or female gametes, a fertilised oocyte, an unfertilised egg and the combination thereof, wherein said housing (11) is provided with a wall made of a biocompatible material. The wall is provided with a series of perforations (12, 12') whose size is sufficient in order to bring the intra-uterine medium into a cellular contact with the housing (11) and to keep the elements therein. The inventive device is provided with a system for loading and unloding one or several elements selected from the group containing an embryo, male and/or female gametes, a fertilised oocyte, an unfertilised egg and the combination thereof.

The invention provides a hatching egg sorting tool. The hatching egg sorting tool comprises two tongs arms, finger placing rings arranged at the tail ends of the tongs arms and a rotary shaft, wherein an upper tongs plate is arranged at the front end of one of the tongs arms, a lower tongs plate is arranged at the front end of the other tongs arm, a battery case is arranged on the lower portion of the lower tongs plate, an LED see-through lamp is arranged in the middle of the lower tongs plate and electrically connected with the battery case, the battery case is integrated with the lower tongs plate during manufacturing, and the two tongs arms are connected through the rotary shaft; a viewing window is arranged in the middle of the upper tongs plate and located above the LED see-through lamp, and the viewing window is integrated with the upper tongs plate during manufacturing; the LED see-through lamp is red or blue; a scattering cover is mounted at the front end of the LED see-through lamp. The hatching egg sorting tool is simple in structure, convenient to use, capable of effectively determining whether an egg is fertilized or not and capable of facilitating the classification of fertilized eggs and unfertilized eggs.