The present invention provides a primer set and method for analyzing the nanos1
gene of
Macrobrachium rosenbergii, relating to the
biotechnology field of reproductive-related genes in
Macrobrachium rosenbergii. The Nanos1
gene obtained by the present invention has a cDNA sequence of 2811 base pairs (bp) long, encoding 243 amino acids. Nanos1 is specifically expressed in the
ovary, and the expression level of nanso1 mRNA is highest in
unfertilized eggs, significantly higher than after fertilization and at all stages of embryonic development. During embryonic development, expression is highest during fertilization, significantly higher than during the cleavage stage and extremely significantly higher than during the late embryonic development stage. The
gene'
s expression level during the cleavage stage is significantly higher than from the
blastocyst stage to the larvae stage; however, the expression level is lower and does not differ between the
blastocyst stage and the larvae stage. Nanos1 mRNA is expressed in the
cytoplasm of oogonia and primary oocytes (Oc1, Oc2, Oc3, and Oc4). Nanos1 is closely related to the development of female germ cells in
Macrobrachium rosenbergii and plays an important role in the reproductive development of
Macrobrachium rosenbergii, providing a theoretical basis and ideas for research on reproductive development and sex identification in
Macrobrachium rosenbergii.