53 results about "Unfertilized Eggs" patented technology

The invention relates to a method for cultivating hybridized and backcrossed marine product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten purpuratus and Argopecten irradians, and synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten irradians; inducing the hybridized first filial generation individuals to spawn by using a conventional shade drying and temperature stimulation method so as to obtain unfertilized eggs; simultaneously, stimulating the Argopecten irradians to spawn and discharge seminal fluid, and filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten irradians; and fertilizing the hybridized first filial generation eggs with the seminal fluid of the Argopecten irradians, cultivating according to a conventional method after hatching germ cells, and thus obtaining the hybridized and backcrossed marine product fries which are above 1.5 cm in shell height and used for commercial cultivation. The hybridized and backcrossed marine product fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed marine product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed marine product fries can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

Provided is a method and system for screening chemical compounds or compositions, wherein replicating entities are introduced into the yolk of an (un)fertilized egg or embryo. The method may be extended to elucidate the mechanism-of-action of functional chemical compounds or compositions in the same method and system. The method and system may also be employed for identifying marker genes, marker proteins or marker metabolites.

A hatching egg inspection apparatus includes a loader unit, an inspection unit for determining the viability or the like as to whether a hatching egg is an unfertilized egg or a growth-stopping egg, a rejection unit for rejecting a hatching egg determined to be an unfertilized egg or the like, and an unloader unit for sending a hatching egg toward the next step. The inspection unit is provided with a light emitting unit for emitting prescribed light toward the hatching egg, a cap attachment unit through which the light having been emitted to the hatching egg and having transmitted through the hatching egg passes, and a light receiving unit for receiving the light that has passed through the cap attachment unit. The cap attachment unit includes a cylindrical cap coming into contact with the hatching egg, and a plate to which the cap is attached.

The invention relates to an egg pigeon artificial incubation method, which belongs to avian hatching. The invention is characterized by six steps: firstly, pigeon eggs, which are yielded at the same period, are disinfected, and then put into a hatching machine; secondly, the hatching machine is started, and the temperature inside an incubation room is controlled within 38.2 DEG C to 38.5 DEG C, and the humidity is controlled within 60 percent to 65 percent; thirdly, the eggs are turned over, and the eggs are turned over once every two hours in the process of incubating, and the turning over of the eggs should be stopped one day before squeakers are hatched; fourthly, the eggs are checked, and the first check should be carried after the eggs are incubated for 4-5 days, and the second check should be carried after the eggs are incubated for 9-10 days, and the unfertilized eggs and the addle eggs should be winkled; fifthly, the breeding eggs are shifted from incubation room to hatching room when the eggs are incubated for 16 days; sixthly, the eggs are hatched, and the breeding eggs can be hatched when the breeding eggs are incubated for 18 days, and the temperature inside the hatching room should be 38 DEG C, and the humidity should be within 70 percent to 75 percent. The invention improves the incubation rate and creates condition for pigeon eggs breeding squabs in bulk. The invention shortens the egg product period of egg pigeons. The natural incubation period is 45 days, the artificial incubation period is shortened to about 15 days and the period is improved 66.67 percent.

The invention provides a method for discriminating unfertilized eggs before incubation on the basis of a visible near infrared spectrum. The method comprises the following steps: (1) preparing a to-be-detected sample and acquiring spectral information of the to-be-detected sample; (2) selecting a representative modeling sample by a mathematical analysis method; (3) determining a discrimination model of the unfertilized eggs before optimal incubation; and (4) discriminating the to-be-detected eggs by the discrimination model of the unfertilized eggs before optimal incubation. The invention alsoprovides a device for detecting the unfertilized eggs before incubation on the basis of the visible near infrared spectrum so as to implement the above method. The experiment proves that the accuraterate of discriminating an unknown sample by the optimal discrimination model which is determined by the method for discriminating the unfertilized eggs before incubation based on the visible near infrared spectrum, which is provided by the invention, is 94.77 percent.

The invention relates to a method for cultivating hybridized and backcrossed marine product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten purpuratus and Argopecten irradians, and synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten irradians; inducing the hybridized first filial generation individuals to spawn by using a conventional shade drying and temperature stimulation method so as to obtain unfertilized eggs; simultaneously, stimulating the Argopecten irradians to spawn and discharge seminal fluid, and filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten irradians; and fertilizing the hybridized first filial generation eggs with the seminal fluid of the Argopecten irradians, cultivating according to a conventional method after hatching germcells, and thus obtaining the hybridized and backcrossed marine product fries which are above 1.5 cm in shell height and used for commercial cultivation. The hybridized and backcrossed marine productfries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed marine product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed marine product fries canbe cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

The invention discloses a method for increasing the artificial incubation rate of tilapia mossambica. The method comprises the following steps of rinsing fertilized eggs with physiological saline; soaking the rinsed fertilized eggs with levothyrocine T4 solution; incubating the soaked fertilized eggs in running water in which the levothyrocine T4 solution with the concentration of 0.05ppm is added. The method disclosed by the invention has the beneficial effects that unfertilized eggs and eggs with a poor fertilizing effect can be rejected by rinsing, soaking and incubating; meanwhile, the fertilized eggs can absorb levothyrocine T4 from the outside environment, so that the requirement on the levothyrocine T4 during incubation can be ensured, and the incubation rate of the fertilized eggs can be increased. During artificial incubation of the tilapia mossambica, the artificial incubation rate and the fry survival rate are increased, and the yield of fries is increased.

The invention relates to a cynoglossus semilaevis triploid fry mass inducing method which comprises an inducing method and an authenticating method of a triploid fry. The inducing method comprises the steps of collection and insemination of an unfertilized egg, confirmation of triploid induction starting time and the confirmation of hydrostatic shock pressure and processing time; and the authenticating method comprises the steps of authentication with a ploidy tester and the analyzing authentication of chromosome. The method for producing cynoglossus semilaevis triploid fries massively by adopting hydrostatic induction is established at first; the proper start time and the hydrostatic shock condition of triploid induction are screened; and the triploid fry mass inducing method is established. The inducing rate of the triploid fry obtained by the method disclosed by the invention is as high as more than 95 percent. The method established by the invention has the characteristics of advancement, high efficiency, accuracy and reliability, has important application value in cynoglossus semilaevis triploid induction and sterile seedling production and has broad popularization and application prospect in fish cultivation and breeding.

The invention relates to the field of hepialus lagii egg screening technologies, in particular to a hepialus lagii egg screening device. The hepialus lagii egg screening device comprises a box and a scattering device located above the box. A rotatable adsorption barrel with static electricity is arranged in the box. A hole is formed in the top end of the box, a discharging channel of the scattering device stretches into the box through the hole, and a discharging port of the scattering device is located above the adsorption barrel. An egg receiving case with at least two boxes is arranged at the bottom of the box, and cord fabric is arranged on the inner wall of the box and covers the egg receiving case. According to different adsorption forces between the adsorption barrel with static electricity and impurities, broken eggs, unfertilized eggs, non-full fertilized eggs and full fertilized eggs, different substances will fall to different positions of the cord fabric of the egg receiving case under the rotating effect of the adsorption barrel and the gravity of the substances, and therefore the eggs can be screened.

An object of the invention is to effectively prepare a fish embryo with a correct chromosomal ploidy by nuclear transplantation in which an exogenous fish nucleus is transplanted in a cytoplasmic recipient. For this object, the invention comprises a step of preparing a fish embryo by transplanting a fish cell nucleus to an unfertilized egg. The step of preparing a fish embryo comprises a step of imposing physical and / or chemical stress to the unfertilized egg after activation. By imposing such stress, the stage of haplosis in a female nucleus which happens at the early stage of a series of developmental steps occurring in an unfertilized egg is suppressed and the correct ploidy of an obtained embryo is at least secured.

The present invention relates to an automatic egg examining device for differentiating between fertilized eggs and unfertilized eggs, comprising emission means (3), which comprise, for each egg to be examined, at least one coherent laser source forming a coherent optical beam (31) directed at an egg (9) to be examined, reception means (4), which receive the light flux passing through the egg, and data processing means (7), which process the light flux received by said reception means in order to determine the state—fertilized or unfertilized—of the egg.

The invention provides a method and a device for detecting fertilization of hatching eggs before hatching based on a near infrared spectrum. The method comprises the following steps: acquiring a plurality of near infrared spectra formed by carrying out near infrared scanning on hatching eggs in a preset mode, wherein the preset mode refers to scanning each of at least two different preset positions of the hatching eggs for at least one time; acquiring the absorbance at each preset waveband in each of the plurality of near infrared spectra, and calculating an average value of the absorbance ateach preset waveband according to the absorbance at each preset waveband in each spectrum; according to the average value of the absorbance at each preset waveband and a preset weight coefficient of the absorbance at each preset waveband, calculating a predicted value for determining whether the hatching eggs are fertilized by using a preset hatching egg fertilization prediction model; according to the predicted value, determining whether the hatching eggs are fertilized. The method and the device provided by the invention can accurately determine the type of fertilized eggs and unfertilized eggs before hatching without damaging the integrity of the hatching eggs.

The invention provides an application method for a hatching egg sorting tool. the method comprises the following steps of A, turning on the LED tankoscope of a lower clamp plate, opening a finger ring to spread out an upper clamp plate and the lower clamp plate at the front end of a clamp arm, and conducting the egg clamping operation; B, closing the finger ring, supporting two clamp arms by positioning columns at the tail end of the clamp arms, avoiding the excessive closing of the finger ring, and avoiding the breakage of an egg between the upper clamp plate and the lower clamp plate, wherein the egg is located between the upper and lower clamp plates at the moment; C, through the perspective window of the upper clamp plate, observing the e internal condition of the egg, and judging the egg is fertilized on the condition that black points occur near the internal yolk portion of the egg under the mapping effect of the LED tankoscope; D, selecting the LED tankoscope to be red or blue. The front end of the LED tankoscope is additionally provided with a scattering cover. The method disclosed by the invention is simple and convenient to use. Based on the method, whether an egg is fertilized or not can be effectively distinguished. Therefore, unfertilized eggs and fertilized eggs are conveniently classified.

The invention relates to a quality detection method for an unfertilized egg of turbot. When the RNA (Ribose Nucleic Acid) concentration of the unfertilized egg of the turbot is 780+ / -0.001mu g / egg and the ratio of Pr to RNA to DNA (Deoxyribose Nucleic Acid) is (227-250) to (12-13.5) to (1-1.2), the fertility rate and the hatchability are highest and the quality of the egg is optimal at the same time. According to the quality detection method disclosed by the invention, the quality of turbot eggs can be quickly and effectively detected, thereby quickly determining whether the eggs to be researched meet the requirement demand and increasing the production efficiency; and meanwhile, the quality evaluation system of teleostean eggs is further completed, and an effective platform and an reference are provided for the quality evaluation for researching other cultivated seawater fish eggs.

Provided are a method for preparing a mammalian ovum or embryo in which zona pellucida has been thinned or eliminated, and a method for fertilization using the mammalian ovum prepared by the aforementioned method. The method for thinning or eliminating zona pellucida of a mammalian ovum or embryo involves treating or culturing the mammalian ovum or embryo (such as an unfertilized ovum, a fertilized ovum, or an embryo in the early stages of development) in a culture medium containing a reducing agent having SH groups (such as reduced glutathione or DTT). The resulting mammalian ovum or embryo (such as an unfertilized ovum, a fertilized ovum, or an embryo in the early stages of development) in which zona pellucida has been thinned or eliminated is capable of realizing an improved fertilization rate and development rate when used for in vitro fertilization, transplantation of a fertilized ovum, or preparation of an embryo in the early stages of development used in the production of a genetically modified animal.

The invention discloses a method for inducing silkworm meiosis type parthenogernesis by liquid refrigerants, belonging to the field of insect genetic breeding technology. The method disclosed by the invention comprises the following steps: (1), collecting and cleaning unfertilized eggs; (2), classifying and preparing the liquid refrigerants; (3), performing cold shock on the unfertilized eggs; and (4), protecting the unfertilized eggs subjected to cold shock and hastening the hatching of the silkworm eggs. According to the invention, a silkworm meiosis type parthenogernesis inducing test achieves success by taking the liquid refrigerants as a medium and Chinese silkworms and Chinese-Japanese crossbreeding silkworms as materials, male silkworm individuals in a genotype homozygous type are obtained, and a new approach is provided for obtaining novel homozygous and true hybrid genetic germplasm resources of Chinese silkworms, and further developing research and utilization of gene expression, excellent character formation, heterosis mechanisms and the like.

The invention discloses a method for preparing sialoglycopeptide (SGP) by large-scale separation and purification. The method comprises (1) taking fresh unfertilized egg yolk, treating with phenol, centrifuging to obtain a supernatant and lyophilizing to obtain coarse sialoglycopeptide; (2) filling a cotton hydrophilic chromatographic column with medical degreasing cotton; (3) adding a solution ofthe crude sialoglycopeptide to the cotton hydrophilic chromatographic column; (4) conducting gradient elution on the cotton hydrophilic chromatographic column with acetonitrile to remove salt and impurities and conducting elution and lyophilization on the glycopeptide with water to obtain pure sialoglycopeptide. According to the method, the cumbersome steps of Sephadex G-50 separation, Sephadex G-25 desalination, ion exchange chromatography purification and the like are omitted, the reaction conditions are mild, the experiment cost is reduced, the purification cycle is shortened, the efficiency is improved, and the purity of the sialoglycopeptide is 95% or above according to the purity detection. The method is not only suitable for enrichment of laboratory glycopeptide but also used for industrial production.

The invention discloses a method of Platichthys stellatus Pallas reduction gynogenesis with induction of pressure shock, comprising the steps: (1) establishing sperm cold storage for perches, (2) inactivating sperm genetic substances, (3) collecting and impregnating unfertilized eggs of Platichthys stellatus Pallas, (4) conducting induction of pressure shock towards fertilized eggs, and (5) cultivating gynogenetic eggs.The method of Platichthys stellatus Pallas reduction gynogenesis with induction of pressure shock has the advantages of improving the fertility rate and developmental rate of Platichthys stellatus Pallas's gynogenesis and establishing gynogenetic groups of Platichthys stellatus Pallas.

The present invention provides novel methods for improving the efficiency of artificial activation of unfertilized mammalian oocytes by reducing the intracellular concentration of Zn2+ in the oocyte. The methods of the invention may additionally comprise a preceding step of increasing the intracellular concentration of Ca2+ in the oocyte prior to reduction of the intracellular Zn2+ concentration. The invention further provides unfertilized oocytes activated by the disclosed methods and viable mammalian animals produced from unfertilized oocytes activated by the disclosed methods.

The invention discloses an immunization method of chicken embryos, comprising the following immunization steps of (1) sealing, steaming and disinfecting the immunization space with potassium permanganate and formalin in each cubic meter; (2) disinfecting the chicken embryo air-chamber with alcohol of which the concentration is 75%; (3) candling the chicken embryos of which the age is 18-19 days to screen the dead eggs or the unfertilized eggs; (4) diluting the immunization vaccine with 200-220 milliliters of diluent in each 4000 dose; (5) immunizing the single strain with the immunization chicken embryo with the diluted vaccine, wherein the immunization part is the air-chamber part of the chicken embryo; and (6) sealing, which is to seal the immunization needle holes of the chicken embryo eggs which are immunized. In the invention, the immunization space and the immunization parts are firstly disinfected, so the infection possibility is reduced, and the temperature and the humidity of the immunization space are stabilized; and because of the immunization sealing, the immunization effect is ensured, the stress death of the chicken embryos is prevented, and the hatch loss is reduced.

The invention discloses a control method of sexuality of Gekko japonicus juveniles. The control method comprises the following steps of 1) going through the appearances and the colors of collected Gekko japonicus ova, selecting the ova, which are provided with a complete fragile egg shell and is ivory and faint pink, and removing unfertilized ova and the ova with the formed embryos; 2) placing the Gekko japonicus ova, which conform to the requirements, into a plastic incubation box provided with a cover, laying damp vermiculites with the thickness of 1-1.5 cm at the bottom of the box, and burying the half of the ova into the damp vermiculites; 3) setting the temperature of the plastic incubation box within the scope of 28-32 DEG C, and inoculating in a constant temperature room for 40-56 days. According to the control method, the Gekko japonicus juveniles with the required sexuality are obtained through controlling the inoculation temperature and dropping a letrozole anhydrous alcohol solution.

The invention provides a debonding and hatching method for fertilized eggs of Thamnaconus septentrionalis, which belongs to the field of aquaculture and comprises the following steps: dissolving alkaline protease from bacillus licheniformis in seawater to prepare a 5g / L debonding solution; collecting fertilized eggs of the Thamnaconus septentrionalis, putting the fertilized eggs into a debonding solution, continuously stirring and uniformly mixing, stopping debonding when the fertilized eggs are not adhered to each other, are adhered to the barrel wall and are in a certain bright and transparent state, and separating the fertilized eggs from unfertilized eggs by increasing the salinity of seawater from the debonded fertilized eggs; washing the separated fertilized eggs clean and then hatching according to a normal procedure. According to the method, germ pollution in the hatching and seedling raising processes can be reduced, and the hatching rate and the emergence rate are increased.

An object of the invention is to effectively prepare a fish embryo with a correct chromosomal ploidy by nuclear transplantation in which an exogenous fish nucleus is transplanted in a cytoplasmic recipient. For this object, the invention comprises a step of preparing a fish embryo by transplanting a fish cell nucleus to an unfertilized egg. The step of preparing a fish embryo comprises a step of imposing physical and / or chemical stress to the unfertilized egg after activation. By imposing such stress, the stage of haplosis in a female nucleus which happens at the early stage of a series of developmental steps occurring in an unfertilized egg is suppressed and the correct ploidy of an obtained embryo is at least secured.

Methods are provided for delivery of at least one agent into egg(s) from an egg-producing aquatic animal including contacting fertilized or unfertilized egg(s) from said egg-producing aquatic animal with the at least one agent in the presence of a guanidine-containing compound capable of enhancing the permeability of the chorion of the egg(s). Methods are provided for the drug screening and compound toxicity assays. Methods are also provided for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of agents that lead to the failure of fertile gonadal development. Agents may be delivered to the eggs directly before the fertilization or post fertilization by contacting eggs in an immersion medium including the agent of interest.

The invention relates to a method of generating transgenic animals, both vertebrates and invertebrates, but preferably vertebrates and, more preferably, non-human mammals, for exogenous DNA sequences or transgenes with variable dimensions. The inventive method is characterized in that it comprises generation from transgenic embryos by means of co-microinjection of sperm or sperm heads (in the case of mice) which are bound to the exogenous DNA sequence of interest in non-fertilised oocytes. The invention also relates to the non-human transgenic animals thus obtained.

The invention relates to a cynoglossus semilaevis triploid fry mass inducing method which comprises an inducing method and an authenticating method of a triploid fry. The inducing method comprises the steps of collection and insemination of an unfertilized egg, confirmation of triploid induction starting time and the confirmation of hydrostatic shock pressure and processing time; and the authenticating method comprises the steps of authentication with a ploidy tester and the analyzing authentication of chromosome. The method for producing cynoglossus semilaevis triploid fries massively by adopting hydrostatic induction is established at first; the proper start time and the hydrostatic shock condition of triploid induction are screened; and the triploid fry mass inducing method is established. The inducing rate of the triploid fry obtained by the method disclosed by the invention is as high as more than 95 percent. The method established by the invention has the characteristics of advancement, high efficiency, accuracy and reliability, has important application value in cynoglossus semilaevis triploid induction and sterile seedling production and has broad popularization and application prospect in fish cultivation and breeding.