The invention discloses a Bacillus methylotrophicus strain VJ4-1 of which the preservation number is CCTCC No:M 2012004. The invention provides a tobacco angelica sinensis extract prepared by using microbe Bacillus methylotrophicus and conducting biotransformation and an application of the angelica sinensis extract in tobacco. According to the invention, by applying the fermented and purified tobacco angelica sinensis extract in cigarettes, the extract is in harmony with the smoke, increases the sweet aroma and the sweetness after taste, reduces the irritancy, reduces miscellaneous gases, strengthens the penetrability, makes the smoke soft and smooth, reduces the drying degree, make the aftertaste good, and improves the tasting; the method has the advantages of simple process, mild reaction conditions, low pollution, high concentration of the products, simple extraction steps, clean production, environmentally friendly product, safety and reliability, wide sources of main raw materials, extremely low cost, and nature, and has an industrial production prospect.