Preparation method and application of Bacillus methylotrophicus and tobacco angelica sinensis extract thereof

A technology of eating methanol spores and angelica extract, which is applied in the field of tobacco flavors, can solve the problems of dry throat, many by-products, and large irritation, and achieve the effect of wide sources of raw materials, wide sources, and simple process

Inactive Publication Date: 2013-01-02
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The plant extracts currently used in cigarettes have major defects such as serious residues and high irritation, which greatly restricts their application in cigarettes
For example, CN101624555A disc

Method used

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  • Preparation method and application of Bacillus methylotrophicus and tobacco angelica sinensis extract thereof
  • Preparation method and application of Bacillus methylotrophicus and tobacco angelica sinensis extract thereof
  • Preparation method and application of Bacillus methylotrophicus and tobacco angelica sinensis extract thereof

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Experimental program
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Effect test

Embodiment 1

[0033] The present invention utilizes the microorganism Bacillus methylotrophicus VJ4-1CCTCC NO: M 2012004 to biotransform the preparation method of Angelica extract for tobacco as follows:

[0034] 1) Crush the Chinese herbal medicine Angelica sinensis to 30 mesh, then reflux extract the coarse powder of Angelica sinensis and water at a mass ratio of 1:20 for 3 hours, then filter, repeat the reflux extraction filtrate once, combine the filtrates and sterilize at 115°C for 25 minutes;

[0035] 2) Slant culture: inoculate Bacillus methylotrophicus VJ4-1CCTCC NO: M 2012004 on the potato dextrose agar slant medium, and culture it statically for 72 hours at 25°C and pH 12, then place it in a refrigerator at 4°C;

[0036] Slant medium: potato infusion 200mL / L, glucose 20g / L, agar 13g / L;

[0037] 3) Preparation of seed culture medium: use the slant culture obtained in the above step 2) to inoculate the seed culture medium, vibrate for 12 hours at 25°C and rotate at 150 rpm, and tra...

Embodiment 2

[0042] The present invention utilizes the microorganism Bacillus methylotrophicus VJ4-1CCTCC NO: M 2012004 to biotransform the preparation method of angelica extract for tobacco as follows:

[0043] 1) Crush the Chinese herbal medicine Angelica sinensis to 50 mesh, then reflux extract the coarse powder of Angelica sinensis and water at a mass ratio of 1:40 for 2 hours, then filter, repeat the reflux extraction filtrate twice, combine the filtrates and sterilize at 115°C for 25 minutes;

[0044] 2) Slant culture: inoculate Bacillus methylotrophicus VJ4-1CCTCC NO: M 2012004 on the potato dextrose agar slant medium, and culture it statically for 36 hours at 30°C and pH 10, then place it in a refrigerator at 4°C;

[0045] Slant medium: potato infusion 200mL / L, glucose 20g / L, agar 13g / L;

[0046] 3) Preparation of seed culture solution: use the slant culture obtained in the above step 2) to inoculate the seed medium, vibrate and culture at 30°C and 120 rpm for 24 hours, and make t...

Embodiment 3

[0051] The present invention utilizes the microorganism Bacillus methylotrophicus VJ4-1 CCTCC NO: M 2012004 to biotransform the preparation method of Angelica sinensis extract for tobacco as follows:

[0052] 1) Crush the Chinese herbal medicine Angelica sinensis to 80 meshes, then reflux extract the coarse powder of Angelica sinensis and water at a mass ratio of 1:60 for 1 hour, then filter, repeat the reflux extraction filtrate 3 times, combine the filtrates and sterilize;

[0053] 2) Slant culture: inoculate Bacillus methylotrophicus VJ4-1CCTCC NO: M 2012004 on the potato dextrose agar slant medium, and culture it statically for 24 hours at 35°C and pH 8, then place it in a 4°C refrigerator;

[0054] Slant medium: potato infusion 200mL / L, glucose 20g / L, agar 13g / L;

[0055] 3) Preparation of seed culture medium: use the slant culture obtained in the above step 2) to inoculate the seed culture medium, vibrate and culture at 35°C and 100 rpm for 36 hours, and make the seed c...

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Abstract

The invention discloses a Bacillus methylotrophicus strain VJ4-1 of which the preservation number is CCTCC No:M 2012004. The invention provides a tobacco angelica sinensis extract prepared by using microbe Bacillus methylotrophicus and conducting biotransformation and an application of the angelica sinensis extract in tobacco. According to the invention, by applying the fermented and purified tobacco angelica sinensis extract in cigarettes, the extract is in harmony with the smoke, increases the sweet aroma and the sweetness after taste, reduces the irritancy, reduces miscellaneous gases, strengthens the penetrability, makes the smoke soft and smooth, reduces the drying degree, make the aftertaste good, and improves the tasting; the method has the advantages of simple process, mild reaction conditions, low pollution, high concentration of the products, simple extraction steps, clean production, environmentally friendly product, safety and reliability, wide sources of main raw materials, extremely low cost, and nature, and has an industrial production prospect.

Description

technical field [0001] The invention relates to the technical field of spices for tobacco, in particular to a method for preparing Bacillus methanolate and angelica extract for tobacco. Background technique [0002] In tobacco products, the aroma and taste of tobacco are very important. Adding spices is a key link in the cigarette process, and it is also an effective means to improve the product style and improve the quality of cigarette aroma. Tobacco flavors can be divided into chemical synthetic flavors and natural flavors. In recent years, natural spices, especially Chinese herbal medicine extracts, have become the focus and hotspot of ingredient research and development because of their Chinese characteristics, natural harmlessness, and easy availability of raw materials. [0003] Angelica (Radix Angelicae Sinensis), also known as Qingui, Yungui, etc. At present, the medicinal material Angelica sinensis refers to the root of the Umbelliferae plant Angelica sinensis. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A24B3/12C12R1/07
Inventor 陈义坤魏敏罗诚浩宋旭艳李冉柯炜昌
Owner HUBEI CHINA TOBACCO IND
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