35 results about "Nicotine tobacco" patented technology

A method for the administration of nicotine, THC, tobacco, cannabidiol or a base alkaloid includes administering an absorption conditioning unit having at least two agents selected from the group consisting of (a) a buffer agent, (b) a capturing agent, (c) a penetration agent, and (d) a thermal agent, to the mammal, and then administering by inhalation a bioactive agent selected from the group consisting of nicotine, THC, cannabidiol and a base alkaloid. The absorption conditioning unit may be in a dosage form not containing a drug. The absorption conditioning unit may create a pH in the oral cavity of 7.8-10 for a period of ten minutes or more after administration, the dosage form not containing an acid and not containing a drug.

The invention discloses a high-nicotine tobacco extract and application thereof, and belongs to the field of tobacco additives. The extract is rich in aroma components such as megastigmatrienone, damascone, pyrones and furanones in tobacco, can provide tobacco aroma and roasting aroma, further contains certain nicotine, can cater for physiological needs of consumers, and improves satisfaction. Content of macromolecular substances such as proteins, pectin and polysaccharides in the extract is extremely low, solubleness is good, the high-nicotine tobacco extract can be completely dissolved into MCT (medium chain triglyceride), and can be used for perfuming a filter tip. Besides, the high-nicotine tobacco extract is added into cut stems or reconstituted tobaccos, so that stimulus can be reduced, strength is improved, a blending rate, in a tobacco group, of the cut stems or reconstituted tobaccos is increased, and content of cigarette tar is reduced while fragrance is invigorated and impurities are covered.

The invention discloses E-cigarette nicotine liquid and a preparation method thereof and belongs to the technical field of E-cigarette nicotine liquids. The E-cigarette nicotine liquid is made from high-nicotine tobacco extract, tobacco flavor and the like, wherein the tobacco extract has rich tobacco characteristic fragrant ingredients (such as megastigmatrienone, beta-damascone, pyrones, and furanones) and nicotine, can provide tobacco aroma and curing aroma and meet the physiological needs of consumers, and provides better satisfaction. The high-nicotine tobacco extract has very low contents of high-boiling-point ingredients and macromolecular substances (such as proteins, pectin, and polysaccharides), is free of burnt smell and low in sweetness, and can supplement aroma and cover peculiar odors, lower stimulus and improve taste strength. By being used with tobacco flavors, the E-cigarette nicotine liquid has enhanced curing aroma and tobacco aroma, and has strong aroma and good volatility. The preparation process of the E-cigarette nicotine liquid is simple, is simple and easy to perform, and is applicable to large-scale industrial production and application.

The invention relates to a method for early-stage screening of high-nicotine and low-nicotine tobacco converter plants. The method mainly comprises the steps of taking aseptic seedling leaves from indoor tobacco seed sprout, adopting a CTAB small-sample method to extract tobacco genome DNA, using the DNA as a template, using a nicotine converted specific primer to perform PCR amplification, adopting 2% agarose gel electrophoresis to separate and show a PCR amplification product, observing, reading and counting molecular marker band spectrums in a gel-imaging system and comparing the band spectrums with a contrast characteristic band spectrum to screen high-nicotine and low-nicotine conversion materials. The method is not limited by environment conditions and seasonal influence, does not need artificial chemical inducted tobacco conversion, high-nicotine and low-nicotine converter plant screening is performed before sowing, and time cost and economic cost are greatly saved. In addition, the method is accurate, efficient, simple and convenient to operate and easy to centralize and popularize, can be used for screening of high-nicotine and low-nicotine conversion materials of different types of tobaccos and can be also used for quick purification and identification performed according to specific nicotine conversion characters.

The invention discloses a method for rapidly screening high-nicotine tobacco mutants, and belongs to the biotechnical field. The method comprises the following steps: 1) dibbling tobacco M2-generation seeds onto an aqueous methyl jasmonate solution; 2) 15 days later, screening single plants of which root lengths are significantly suppressed; 3) transferring the single plants screened in the step 2) to an MS culture solution, and culturing for 20 days; 4) transferring the tobacco plants in the step 3) to a provisonal planting plate containing nutrient soil, and 30 days later, transplanting to a field; 5) performing field planting and routine management; 6) determinating nicotine contents at three different time points; 7) selfing and harvesting seeds. According to the method, within 20 days, high-nicotine materials are screened from 4000 China tobacco-100 mutants, and 5 high-nicotine flue-cured tobacco materials are obtained; the highest nicotine content reaches 7.09%, which is the highest nicotine content for current flue-cured tobacco; the efficiency for screening the tobacco mutants is greatly improved, the workload for screening the high-nicotine tobacco is reduced, and the production efficiency is improved.

The invention discloses liquid sea salt nicotine. The formula of the liquid sea salt nicotine comprises, by mass, the following components: 1, 250 to 350 parts of basic organic acid; 100 to 150 partsof alcohol-soluble organic acid; 350-450 parts of nicotine, 100-150 parts of absolute ethyl alcohol, 100-150 parts of sea salt, 100-150 parts of distilled water, 10-20 parts of menthol, 10-20 parts ofxylitol, 2-5 parts of natural sweetening agent, 50-70 parts of propylene glycol, 50-70 parts of glycerol, 1-3 parts of mint extracting solution and the like. The sea salt, the sweetening agent and various natural plant extracting solutions are added into tobacco tar, the taste of the tobacco tar smoked by a smoker can be improved, and therefore excessive stimulation of nicotine tobacco juice to the throat is reduced; citric acid, ascorbic acid and the like are added into organic acid components of the sea salt nicotine, the alcohol-soluble organic acid can increase the concentration of smoke,the basic organic acid and an acid-base value of the alcohol-soluble organic acid are matched to adjust the whole acid-base value, the smoking experience of the tobacco tar can be improved through the added natural plant extract, the smoker is assisted in relieving smoking addiction, and safety is high.

The invention relates to a method for screening high-nicotine tobacco variety mutants. According to the method, mutagenized plants are created by the EMS mutagenesis method, leaf glandular hair morphology screening and leaf secretion determination are performed to select first mutant strains meeting first set criteria, and M3 generation seeds are harvested for cultivation; the Tilling technology is used to screen mutant single plants of a nicotine synthesis negative regulation gene NtJAZ1, continuous backcross and self-cross are carried out between the mutant single plants and tobacco varieties to be screened as recurrent parents, and mutant strains with high-nicotine and high-secretion are obtained with the help of the molecular marker and gene chip-assisted selection technology; and comparative studies on agronomic traits, comprehensive resistance, chemical composition, sensory quality and the like of the mutant strains are performed, new varieties with increased nicotine content, excellent comprehensive traits, and significantly-improved tobacco aroma are selected from the mutant strains, and thereby the foundation for the production of highly-flavored high-quality tobaccos is laid.

The invention provides a low-nicotine tobacco extract and a preparation method thereof and tobacco tar special for a heat-not-burn cigarette. The preparation method of the low-nicotine tobacco extractcomprises the following steps of: extraction, specifically, adopting an ethanol solution for conducting digestion on tobacco raw materials, performing solid-liquid separation after digestion to obtain leach liquor, and centrifuging the leach liquor to obtain a tobacco extracting solution; concentrating: concentrating the tobacco extracting solution under reduced pressure to obtain a tobacco alcohol extract; alkalization extraction: adding ammonia water into the tobacco alcohol extract, performing uniform stirring, and mixing with an organic solvent for extraction; purification: separating liquid after extraction to obtain an organic phase and a water phase, and mixing the water phase with an ethanol solution, performing uniform stirring, and performing concentrating under reduced pressureto obtain the low-nicotine tobacco extract. The preparation method disclosed by the invention is simple in process requirement, high in yield and suitable for large-batch production. The tobacco extract prepared by adopting the method still has obvious tobacco fragrance while having lower nicotine content, and can be used for preparing heat-not-burn cigarettes.

This invention is related to an oral pharmaceutical composition that contains a cholinergic agent, a natural plant alkaloid in particular, selected from the group of lobeline, anabasine, cytisine, galantamine or their acceptable salts, in the form of tablets and capsules. The excipients of the developed oral composition include cellulose powder, calcium sulphate, silica colloidal and magnesium stearate, the total content of cellulose powder and calcium sulphate dihydrate being from 64,5 to 97,5 % of the mass of the dosage form and at least 90% of the alkaloid particles being < 100um. The oral composition contains also at least one biologically active amino acid selected from: L-carnitine, tryptophan or a combination of them. The oral composition according to this invention achieves uniform distribution of the active substance in the composition, as well as stability of the composition due to the included excipients selected so as to react to a minimum with the alkaloid to form the qualitative and quantitative related substances admissible for the pharmaceutical composition. The composition according to this invention is applicable in the treatment of dependency and addiction to nicotine, tobacco products and alcohol.

The invention discloses a tobacco nicotine metabolism related gene and application thereof. The gene is an NtLNP2 gene, the sequence of the gene is SEQ ID No.1, and after the sequence of the gene is translated, the coded protein sequence is SEQ ID No.2. A method for knocking out the tobacco nicotine metabolism related gene by using a CRISPR/Cas9 system comprises the following steps: (1) designing an sgRNA guide sequence, and constructing an sgRNA expression vector; (2) introducing the expression vector into agrobacterium; (3) infecting the callus; and (4) carrying out a detection test on the nicotine content of the squaring-stage leaves of the NtLNP2 gene homozygous knockout material by GC-MS. The gene and the method provided by the invention provide genetic materials and theoretical basis for tobacco nicotine metabolism gene function research and low-nicotine tobacco new variety cultivation research.

The invention discloses a method for creating a low-nicotine tobacco mutant by knocking out NtBBLs by using CRISPR/Cas9 and application. The method comprises the following steps: (1) constructing a CRISPR/Cas9 gene editing carrier; (2) preparing infected agrobacterium; (3) carrying out genetic transformation to obtain regenerated edited plants and homozygous T1-generation plants; and (4) carrying out a detection test on the nicotine content of leaves of the NtBBLs gene homozygous knockout material in the squaring stage by GC-MS (Gas Chromatography-Mass Spectrometer). According to the application, the NtBBLs gene is knocked out by utilizing a CRISPR/Cas9 mediated gene editing technology, so that a tobacco knockout material which keeps original agronomic characters and meanwhile, the nicotine content is greatly reduced can be obtained.

The invention discloses a preparation method of high-aroma low-nicotine tobacco shreds. The preparation method comprises the following steps: extracting tobacco shreds with a non-polar solvent, a weak-polar solvent, a medium-polar solvent and a polar solvent in sequence, removing solvents from extract solutions of the non-polar solvent, the weak-polar solvent and the medium-polar solvent, respectively backfilling the extracted tobacco shreds with the extract solutions, and performing cut tobacco drying/fluidized drying to obtain the high-aroma low-nicotine tobacco shreds. According to the method, the aroma quality of the tobacco shreds is ensured while the nicotine content is reduced.

The invention provides a high-nicotine tobacco extract, a preparation method thereof and a heat-not-burn cigarette, and the preparation method of the high-nicotine tobacco extract comprises the following steps: mixing crushed tobacco raw materials with water, and adding an enzyme preparation for treatment; mixing the treated tobacco raw material with a high-concentration ethanol solution for leaching, performing solid-liquid separation after leaching to obtain a leaching solution, and filtering the leaching solution to obtain a tobacco clear solution; concentrating the tobacco clear liquid under reduced pressure to obtain a tobacco alcohol extract; adding alkali liquor into the tobacco alcohol extract, uniformly stirring, and mixing with an organic solvent for extraction; separating liquidafter extraction to obtain an organic phase and a water phase, and concentrating the organic phase under reduced pressure to obtain a product, namely the high-nicotine tobacco extract. The nicotine content of the prepared high-nicotine tobacco extract is higher than that of a conventional tobacco extract, and the high-nicotine tobacco extract has obvious tobacco fragrance and strength. The heat-not-burn cigarette added with a small amount of high-nicotine tobacco extract is rich in tobacco fragrance and strength.