A kind of enzyme preparation using nicotine dehydrogenase as auxiliary tobacco processing and its preparation and use method

A technology of nicotine dehydrogenase and alkali dehydrogenase, which is applied in the field of enzyme preparations to improve tobacco grades, can solve the problems of affecting tobacco leaf smoking evaluation results and low nicotine content, and achieve the effect of easy use and reduced nicotine content

Active Publication Date: 2011-12-07
云南万芳生物技术有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, if the nicotine content is too low, the difference in the ratio of total nitrogen / nicotine and reducing sugar / nicotine will affect the results of tobacco leaf smoking evaluation

Method used

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  • A kind of enzyme preparation using nicotine dehydrogenase as auxiliary tobacco processing and its preparation and use method

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0041] (1) Preparation and determination of the preparation of the present invention

[0042] Nicotine dehydrogenase can be purchased directly from microbial fermentation products. Or use the corresponding nicotine dehydrogenase-producing strains to ferment and produce crude products through cell wall breaking, release and preliminary extraction. Then, refine, remove metal ions, dry and concentrate with the method of the present invention to obtain the nicotine dehydrogenation preparation.

[0043] Dissolve and dilute the solid nicotine dehydrogenase crude product with W / V=1g:20mL or liquid W / V=1mL:10mL buffer, the pH value of Britton-Robinsion buffer is 7.0; centrifuge at 3500r / min for 20min, take the Clear solution, add (NH 4 ) 2 SO 4 , so that the final concentration of ammonium sulfate is 62%, let it stand for 24h, then centrifuge at 15000r / min at 4°C for 30min, take the supernatant, and add (NH 4 ) 2 SO 4 Overnight, centrifuge again at 15000r / min ...

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Abstract

The invention relates to an enzyme preparation using nicotine dehydrogenase as an auxiliary for tobacco processing, and a preparation and use method thereof, belonging to the enzyme preparation added before tobacco re-curing to improve the tobacco grade. In the present invention, the known nicotine dehydrogenase enzyme source is removed and refined, and EDTA complexing agent is used to remove metal ions in the enzyme solution, so that the enzyme in the concentrated stock solution does not show enzymatic activity, so as to facilitate the passivation of long-term storage. It exists in a stable form, and is concentrated and prepared by freeze-drying to become an active tobacco additive. When used, it is diluted with water to replace the original second leaf moisturizing water when the cigarette is redried, which can better degrade the amount of nicotine in the tobacco and improve the smoking of tobacco. safety.

Description

technical field [0001] The invention belongs to an enzyme preparation for improving the grade of tobacco added before rebaking of tobacco. Background technique [0002] Microorganisms and enzymes play very important roles during tobacco leaf growth, conditioning, aging, processing and storage. In the mid-1850s, people began to use microorganisms or enzymes to improve the quality of tobacco leaves, tobacco stems and tobacco flakes, produce spices and degrade nicotine, which is still a hot research topic. Nicotine is an alkaloid in common tobacco, commonly known as nicotine (Nicotine), which acts on autonomic nerves, central nervous system and motor nerve endings. The content is directly proportional. With the continuous deepening of the worldwide anti-smoking campaign and consumers paying more attention to their own health, almost all types of cigarette products are developing in the direction of low-tar and low-nicotine light-flavored cigarettes. There are many upper toba...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02A24B15/20C12Q1/32
Inventor 韩伟何宝玉陈应昆
Owner 云南万芳生物技术有限公司
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