80results about How to "Reduce screening workload" patented technology

The invention discloses an omega-transaminase mutant and an encoding gene and preparation method thereof. The amino acid sequence of the omega-transaminase mutant is shown in SEQ ID NO.2 or SEQ ID NO.4 or SEQ ID NO.6 or SEQ ID NO.8 or SEQ ID NO.10. The nucleotide sequence of the omega-transaminase mutant is shown in SEQ ID NO.1 or SEQ ID NO.3 or SEQ ID NO.5 or SEQ ID NO.7 or SEQ ID NO.9. The invention further discloses an expression box, recombinant plasmid and converter containing the gene. Mutation site prediction is conducted on a wild omega-transaminase mutant through the combination of a temperature factor (B-factor) and the energy optimizing strategy, then site-specific mutagenesis is conducted, and the omega-transaminase mutant is prepared. The thermal stability of the selected mutant is higher than that of wild transaminase.

The invention discloses a construction method and application of a promoter library. The construction method includes the following steps that firstly, an oligonucleotide sequence with promoter features is designed according to a conserved region, a semi-conserved region, a random sequence and a transcription initiation locus of a prokaryote promoter; secondly, the oligonucleotide sequence designed in the first step is subjected to annealing and extension to form double strands, the double strands are connected to a carrier with a report gene, and a mutation library of the promoter is obtained; thirdly, the mutation library, obtained in the second step, of the promoter is migrated into a host cell, a flat plate with corresponding resistance is coated with the mutation library, and the promoter library is obtained. By the adoption of the oligonucleotide sequence which is artificially designed and has the promoter features and the technologies of molecule aggregation and extension, the promoter library with ideal promoter intensity span is obtained. The method is quick, efficient, cheap and small in screening workload.

The invention discloses a vector for efficiently labeling zebra fish PGC, and a preparation method and a use of transgenic fish. A Gal4 / UAS transcription activation system is utilized and mRFP is used as a report gene to realize an inductive gene expression regulation technology in the primordial germ cells of zebra fish. The preparation method of the transgenic fish comprises the following steps: 1, respectively constructing activation transgenic line Tg (kop:KalTA4) and an effect transgenic line Tg (UAS:mRFP); and 2, hybridizing Tg (kop:KalTA4) male fish with Tg (UAS:mRFP) female fish to obtain the transgenic fish for efficiently labeling the primordial germ cells of zebra fish. The transgenic fish for efficiently labeling the primordial germ cells of zebra fish can be widely applied to the fish bioengineering.

The invention relates to a chemical mutation breeding technology in the field of biotechnologies, and particularly relates to an in-vitro sodium azide (NaN3) mutation breeding technology of dendranthema morifolium. The in-vitro NaN3 mutation breeding technology comprises the following steps of: performing culture in vitro on an immature stem, a stem tip or a leaf of the dendranthema morifolium to form an aseptic seedling; processing the stem tip and a stem segment of the aseptic seedling, which are taken as explants, by sodium azide (NaN3) in a breeding process to carry out induced mutation; rooting and transplanting a test-tube plantlet; and finally breeding excellent mutant in a field at a flowering phase. The in-vitro sodium azide (NaN3) mutation breeding technology can effectively overcome the obstacle of breed improvement of a clonal plant and the constraints of low bud-variation frequency and small mutational range under natural conditions, can be used for obtaining the excellent mutant within a short period of time, ensures high induced mutation efficiency, and has an important value to the genetic improvement of asexual propagation of the dendranthema morifolium with medicinal and ornamental values.

The invention belongs to the technical field of fermentation engineering and microbial breeding, and discloses a high-throughput screening method for bacillus circulans for generating beta-galactosidase. The high-throughput screening method includes main steps of activating initial strains; carrying out ultraviolet mutation and lithium chloride mutation; carrying out high-throughput screening by the aid of 96 pore plates to obtain bacillus circulans mutant strains with improved enzyme capacity. The high-throughput screening method has the advantages that the mutation probability of the strains can be improved by the aid of the traditional physical and chemical combined mutation modes, the screening workload can be reduced by the aid of high-throughput screening tools, the screening efficiency can be improved, strain breeding procedures can be accelerated, and the optimal strains can be screened.

The invention discloses a novel indica-japonica hybrid gene introgression sheath blight resistance breeding method. The novel indica-japonica hybrid gene introgression sheath blight resistance breeding method includes the following steps that (1), an indica-japonica variety / system to be improved and a sheath blight resistance indica-japonica resistance source are selected; (2), the toxin tolerance concentration of the selected indica-japonica variety / system to be improved is roughly identified; (3), a three-way cross F1 hybrid is acquired through cross breeding; (4), row indica culture medium anther culture is conducted on the three-way cross F1 hybrid; (5), anther-cultured seedlings are planted in a large field, multiple indica-japonica anther culture families with the improved sheath blight resistance are acquired through single-seedling harvesting and planting, the different anther culture families are further sown in the large field, a parent of the indica-japonica variety / system to be improved serves as a contrast, and the new indica-japonica strain with the good agronomic character and the improved sheath blight resistance is screened out. By the adoption of the novel indica-japonica hybrid gene introgression sheath blight resistance breeding method, indica-japonica sheath blight resistance genes can specially and efficiently seep into indica-japonica, sheath blight resistance breeding can be finished about three years, a conventional indica-japonica hybrid sheath blight resistance breeding period is remarkably shortened, and the high breeding application value is achieved.

The invention discloses a Paecilomyces thermophila mutant strain and a mutation induction method and application thereof. The mutation induction method includes: Paecilomyces thermophila screened from high-temperature-fermented compost and used for producing high-temperature-resistant and acid-resistant glucose oxidase is used as the original strain, protoplast ultraviolet and ethyl methane sulfonate compound mutation induction is performed, and the screened strain is subjected to budding spore diethyl sulfate-microwave compound mutation induction to obtain the Paecilomyces thermophila mutant strain. The Paecilomyces thermophila mutant strain is preserved in China General Microbiological Culture Collection Center on January 11th, 2016, and the preservation number of the mutant strain is CGMCC No. 13182. Compared with a conventional mutation induction method, the mutation induction method has the advantages that the method is high in efficiency and simple in fast in screening, the productivity of the mutant strain is high, the yield of the mutant stain is 277.65% of that of the Paecilomyces thermophila original strain, fermentation liquor enzyme activity reaches 185U / ml, production cost is lowered, and the glucose oxidase produced by the mutant strain is good in high-temperature resistance and acid resistance and can be used as feed enzyme applied to feed industry.

The invention discloses a method for improving hydrolase robustness in combination with high-pressure molecular dynamics simulation and free energy calculation, and belongs to the fields of computational chemistry, bioinformatics, genetic engineering and protein engineering. According to the method, high-pressure disturbance is combined with simulation calculation of a molecular dynamics model tosearch for a strong-robustness related region and a key amino acid site, virtual saturation mutation is conducted on amino acid on the key region, and a mutant library is constructed; and then, multi-scale free energy calculation software is used for carrying out stability prediction on the mutants from protein dynamics, protein thermodynamics and prediction algorithms, and false positive mutantsare eliminated as much as possible through multiple rounds of virtual selection. By utilizing the method to construct and screen the hydrolase mutant, the frequency of the positive mutant is as high as 62.5%, the screening workload is effectively reduced, the obtained mutant is strong in robustness, and the method has very high industrial application potential.

The invention relates to a method for breeding a saussurea involucrata plant. In the method, the lower hypocotyl stem section of the saussureae involucratae aseptic seedling after cutting off root and leaves is used as the explant, the explant is induced by using solid culture media with different concentrations of colchicine to generate a tetraploid saussureae involucratae plant, and the tetraploid saussureae involucratae is identified by using the tetraploid screening identification technology. The method provides an improvement method for optimal colchicine concentration, processing time and processing temperature for inducing generation of tetraploid, and morphology, chromosome counting and flow cytometry for identifying tetraploid. The method can be used for quickly breeding the autotetraploid saussureae involucratae plants; and the method shortens the breeding cycle, thereby effectively increasing the content of active ingredients, and overcomes the defect of variety degeneration of the saussureae involucratae in natural propagation and artificial planting.

The invention discloses a new bacterial-blight-resisting japonica rice breeding method. The new bacterial-blight-resisting japonica rice breeding method comprises the following steps that 1, a japonica rice variety / strain to be improved and a bacterial-blight-resisting indica type rice source are selected; 2, coarse toxin tolerance concentration authentication is conducted on the selected japonica rice variety / system; 3, crossbreeding is conduced, and a three-way cross F1 hybrid is obtained; 4, row indica type rice culture medium anther culture is conducted on the three-way cross F1 hybrid; 5, the anther cultured seedlings are planted in a field, a plurality of japonica rice anther cultured strains with improved bacterial blight resistance are harvested and obtained from singular plants, the japonica rice anther cultured strains are further planted in a field, a japonica rice variety / strain parent to be improved is used as a comparison standard, and a new japonica rice strain with good agronomic property and improved bacterial blight resistance is obtained through screening. By the adoption of the new bacterial-blight-resisting japonica rice breeding method, the bacterial blight resistance is specifically and effectively introduced into japonica rice, a bacterial-blight-resisting japonica rice variety can be bred within about three years, the period of conventional bacterial-blight-resisting japonica rice breeding is remarkably shortened, and the new bacterial-blight-resisting japonica rice breeding method has high breeding application value.

The embodiment of the invention discloses a method, a device and a system for screening credit card accounts with cheating transactions, and the method comprises the steps: obtaining a credit card account set which comprises the business feature data of a plurality of credit card accounts; performing clustering processing on the service feature data of the plurality of credit card accounts to obtain a plurality of clustering categories; determining a suspicion degree scoring interval of each clustering category according to the service characteristics of each clustering category and the card number proportion; determining a suspicion score of the credit card account according to the distance between the service feature data of the credit card account and the clustering center of the clustering category where the credit card account is located and the suspicion score interval of the clustering category where the credit card account is located; and screening credit card accounts with cheating transactions in the credit card account set according to the suspicion score. By utilizing each embodiment of the specification, the credit card accounts with cheating transaction behaviors suchas cash register and the like can be screened more accurately and efficiently.

The invention mainly discloses a soil screening machine. According to the technical scheme, the soil screening machine comprises a bottom frame, a feeding port, discharging ports and a screening device. An adjusting inserting plate is arranged at the position of the feeding port. Springs are arranged on the bottom frame. The springs are connected with the screening device. A supporting frame is arranged on the two outer sides of a vibrating frame. A vibration motor is installed on the supporting frame. The two inner sides of the vibrating frame are each provided with at least two first sliding grooves. Vibrating screens are connected into the first sliding grooves in a sliding manner. The hole diameters of screen holes of the vibrating screens on different layers are sequentially reduced from top to bottom. The upper side of the vibrating frame is provided with a dustproof cover. A second sliding groove is formed in the bottom of the dustproof cover. The second sliding groove is provided with a rake connected with the second sliding groove in a sliding manner. An air cylinder is further arranged at the bottom of the dustproof cover. Baffles are arranged on the two sides of the discharging ports. The widths of the discharging ports are gradually reduced from the head portion to the tail portion, and the discharging directions of the discharging ports are different. The soil screening machine has the beneficial effects of being simple in structure, high in screening efficiency, convenient to use and capable of saving cost.

An expression carrier for effectively screening target protein is prepared through introducing an internal ribosome to the eukaryotic expression carrier containing CMV promoter and dhfr amplification system, linking the fusion gene of anti-erbB2-seFv-Fc gene and IL-2 to the neomycin-resistant gene, site mutation to Neo gene by PCR, and configuring the expression carrier pCMV-HFI-mNeo.

The invention relates to a microsphere separation method and a screening system, which are suitable for screening and separating pellets. In the method, a stator plate and a rotor plate are used to form a narrow slit; the impulse stream is injected from the bottom of the narrow slit, and under the disturbing action of periodical starting and stopping of the stream, the microspheres are driven to disperse and pass through the narrow slit are prevented from blocking the narrow slit; and the width of the narrow slit is regulated to realize the screening of the microspheres with different diameters. The system comprises a narrow slit unit, a narrow slit setting and measuring unit, a microsphere disturbance device, a lifting device and a data gathering and controlling unit. The invention has the advantages that the narrow slit is used for screening the microspheres so as to enhance the screening efficiency of the microspheres; the impulse stream method is adopted to fully disperse the microspheres to pass through the narrow slit so as to ensure the screening quality of the microspheres; a high-precision worktable is used to drive the rotor plate so as to ensure the screening precision of the microspheres; and the whole system is controlled by a computer software interface, so that the modification of different kinds of parameter is visible and easy, and the operation is easy.

The invention discloses a method for modifying carbonyl reductase stereoselectivity, a carbonyl reductase mutant and application. The carbonyl reductase stereoselectivity is identified by combining the size and shape of an enzyme with a pocket, the external shape of the pocket of a reverse Prelog enzyme is close to a cube, the external shape of the pocket of the Prelog enzyme is more similar to acuboid, and the enzyme stereoselectivity can be reversed or improved by regulating and controlling key site amino acid. The stereoselectivity (99.5% dep, R) of the carbonyl reductase mutant KmCR-E87D / A129C / V239N prepared by the method is reversed compared with that (99.5% dep, S) of a wild KmCR, and 35.4mM of 6-cyano (3R, 5R)-dihydroxy tert-butyl caproate can be asymmetrically synthesized within10 hours.

The invention provides a method for quickly and efficiently screening a nisin high-yield strain. The method is characterized in that induced mutant lactococcus lactis servers as a starting strain, and low pH-Nisin resistance plate serves as a strain culture carrier for preliminary screening. The method is simple, and required agents are common; by adoption of the low pH resistance plate, screening efficiency is improved greatly through double indexes, a screening range is widened, possibility of screening out of the high-yield mutant strain is increased, and the grown-out strain is more representative; cell growth metabolite is treated to directly prepare a screening culture medium to screen a substrate tolerant strain, a substrate retroinhibition effect of cells is improved, cell growth and metabolism performances are improved, and the obtained strain is superior. By a re-screening step, all strains growing on the preliminary screening plate can be rescreened by one step, screening omission is avoided, and screening workload can be relieved effectively.

The application provides a domain name recognition method, a device and a server. The method comprises the following steps: acquiring a first list, wherein the first list is used for recording a to-be-processed uniform resource locator (URL), and the URL is corresponding to the domain name; computing a page feature value of a page document corresponding to each URL recorded in the first list; determining the URLs with the same page feature value in the first list as the target URL, and taking the domain name corresponding to the target URL as the recognition result. By using the technical scheme provided by the application, the bad website can be discovered without taking the domain name as the feature, the screening workload for discovering the bad website sample is lowered, and the domain name variation is effectively resisted.

The invention discloses a breeding method for rapidly screening resistant varieties of wheat root rot by using root rot fungus crude toxin. The method comprises the following steps of (1) selecting to-be-improved wheat variety / strain and wheat root rot resistance source and hybridizing to obtain F1; (2) backcrossing the F1 to obtain BCF1; (3) sowing male and female parents and the BCF1 at different stages; (4) treating the male and female parents by using root rot fungus crude toxin of gradient concentrations and selecting resistance screening pressure of the root rot; (5) treating the BCF1 by using the resistance screening pressure of the root rot to obtain BCF2; and (6) taking BCF2 for anther culture to obtain an anther culture family and screening according to root rot resistance and agronomic characters to obtain a new wheat strain with root rot resistance. Hybrid species are treated by selecting the root rot fungus crude toxin with a proper concentration at the hypersensitive stage of the root rot fungus crude toxin and the first division stage of zygote, thereby specifically screening the resistance wheat family for the root rot and greatly improving the work efficiency and the accuracy of resistant breeding of the root rot.

The invention relates to a method for breeding a saussurea involucrata plant. In the method, the lower hypocotyl stem section of the saussureae involucratae aseptic seedling after cutting off root and leaves is used as the explant, the explant is induced by using solid culture media with different concentrations of colchicine to generate a tetraploid saussureae involucratae plant, and the tetraploid saussureae involucratae is identified by using the tetraploid screening identification technology. The method provides an improvement method for optimal colchicine concentration, processing time and processing temperature for inducing generation of tetraploid, and morphology, chromosome counting and flow cytometry for identifying tetraploid. The method can be used for quickly breeding the autotetraploid saussureae involucratae plants; and the method shortens the breeding cycle, thereby effectively increasing the content of active ingredients, and overcomes the defect of variety degeneration of the saussureae involucratae in natural propagation and artificial planting.

The invention discloses an equipment operation monitoring method based on a big data technology. The method comprises the following steps: counting enterprise information and enterprise monitoring equipment basic information thereof, and storing the enterprise information and the enterprise monitoring equipment basic information in a cloud platform; monitoring equipment records / stores monitoring video images and online and offline information, sends the monitoring video images and the online and offline information to a cloud platform, generating a monitoring video image and an online state corresponding to a time axis, calculating the current day online duration of each numbered monitoring equipment, obtaining a daily online duration trend line of each monitoring equipment, and summarizing the accumulated online duration of each monitoring equipment at the same time; and enabling the platform interface to display a monitoring video picture, an online / offline state, the online durationof the day, the accumulated online duration and the daily online duration trend chart of the monitoring equipment to the platform registration user. The method has the beneficial effects that the online duration of the kitchen monitoring equipment is uniformly recorded, and the monitoring video picture of the kitchen in the restaurant is displayed in real time.

The invention provides a screening method for erythrocyte osmotic fragility, which comprises the following steps: S1, test solutions of the erythrocyte osmotic fragility are added into an enzyme plate and preheated at a temperature of 37 to 40 DEG C for 1-2 minutes; S2, 5-10 Mu l of anticoagulant whole blood to be measured is added into holding sample holes of partial areas of the enzyme plate, and then the test solution of the erythrocyte osmotic fragility and the anticoagulant whole blood are mixed evenly; S3, test solutions for stopping erythrocyte hemolysis are added into the partial areas filled with the anticoagulant whole blood to be measured, all solutions are mixed evenly and centrifugation is carried out; S4, the supernatant is removed and placed in the holding sample holes of the other partial areas of the enzyme plate; S5, erythrocyte hemolysis solutions are added into the base solution, and all solutions are mixed evenly; S6, after the hemolysis process is finished, colorimetry is carried out between the solution to be measured of the partial areas filled with the erythrocyte hemolysis test solution and the solution of the other partial areas not filled with the erythrocyte hemolysis test solution. With the advantages of simplicity, trace amounts, fast speed and large samples, the screening method for the erythrocyte osmotic fragility further has the advantages that the screening workload can be reduced, the screening can be completed fast and accurately, the reproducible results can be obtained and the experimental results can be automatically calculated.

The invention is applicable to the technical field of microbial fermentation engineering, and provides a high-throughput breeding method of an ultrahigh-concentration beer brewing strain, which comprises the following steps: step 1, obtaining an ultrahigh-concentration strain mutant library by using an ARTP mutagenesis technology; 2, breeding an ultrahigh-concentration brewing strain by using a high-throughput screening method based on a droplet microfluidic technology; 3, MMC micro-fluidic breeding and strain adaptability domestication are carried out; 4, gradient plate breeding; step 5, secondary screening of glucose metabolism power; and 6, analyzing the brewing performance of the optimized strain. According to the method, the number of strains screened each time is effectively increased, the screening period is shortened, the screening working intensity is reduced, the working efficiency and objectivity of the whole screening are improved, and the ultrahigh-concentration brewing strains suitable for the following fermentation are obtained.

The present invention relates to a mutant BtHepID204C / K208C for improving thermal stability of hexotensin I by increasing disulfide bond, and an amino acid sequence of the mutant is SEQ ID NO. 1. Themutant has better thermostability than wild-type heparinase I (BtHepI), and can effectively reduce the screening workload of a mutant library, at the same time, the thermostable heparinase I has greatsignificance for extending the shelf life of heparinase I, improving its operational stability and reusing batches in a catalytic process, , and reducing production costs. The industrial applicationvalue of heparinase I can be improved.

The invention discloses an image scoring method based on deep learning, and the method is characterized in that the method comprises the steps: 1, preparing a picture data set, and carrying out the scoring marking of a picture from different dimensions; 2, training a picture scoring model by using a deep neural network, wherein the picture scoring model is used for evaluating scores of the pictures in more than one designed dimension; 3, carrying out weighted summation on the scores under each dimension to obtain a final score S = sigma(wkScore) of the picture. According to the method, the picture scoring angle of human eyes is referred, the picture scoring model is trained by using deep nerves and the deep neural network, the picture scoring result better meets the human eye judgment standard, the scoring accuracy is greatly improved, the picture screening workload of websites or APPs can be greatly reduced, and the picture click rate of the websites can be increased.

The invention discloses an omega-transaminase mutant and an encoding gene and preparation method thereof. The amino acid sequence of the omega-transaminase mutant is shown in SEQ ID NO.2 or SEQ ID NO.4 or SEQ ID NO.6 or SEQ ID NO.8 or SEQ ID NO.10. The nucleotide sequence of the omega-transaminase mutant is shown in SEQ ID NO.1 or SEQ ID NO.3 or SEQ ID NO.5 or SEQ ID NO.7 or SEQ ID NO.9. The invention further discloses an expression box, recombinant plasmid and converter containing the gene. Mutation site prediction is conducted on a wild omega-transaminase mutant through the combination of a temperature factor (B-factor) and the energy optimizing strategy, then site-specific mutagenesis is conducted, and the omega-transaminase mutant is prepared. The thermal stability of the selected mutant is higher than that of wild transaminase.

The invention discloses a method for fast and efficient screening of a high-yield 5-aminolevulinic acid mutant strain. The method guarantees screening accuracy, reduces screening workload and improves screening efficiency. The method comprises the following steps of mutagenizing a rhodobacter sphaeroides strain by a physical or chemical mutagenesis method, washing the mutagenized strain by a stroke-physiological saline solution, carrying out liquid medium re-suspension, carrying out culture at a temperature of 32 DEG C, carrying out dilution to obtain a bacterial suspension, coating the bacterial suspension on a flat plate, picking a single bacterial colony from the flat plate, transferring it into corresponding holes of a sterile 96-deep well plate, carrying out shaking culture at a temperature of 32 DEG C in the dark for 48h, adding a precursor and succinic acid into the culture products, carrying out re-culture for 24h, and detecting spectrophotometric values at wavelength of 553nm of the bacterial liquid in all the wells by an ALA microscale development method (deep-well plate method), wherein the bacterial liquid having the maximum spectrophotometric value contains the high-yield 5-aminolevulinic acid mutant strain.

The invention discloses an integrated device for peanut drying and peeling of peanuts to prevent blockage of the air suction port. The inside of the inner box is provided with an inner box, and the inside of the inner box is provided with a drying box, the inside of the drying box is provided with a rotating shaft connected with the first motor, and the baffle is symmetrically arranged inside the drying box, The left side of the inner box is connected to the air inlet, and the right side of the inner box is provided with an air suction port, the upper side of the rotating disk is connected to the second motor, and the lower side of the rotating disk is fixed with a stirring rod, The rotating disc and the stirring rod are arranged inside the air suction port, a valve is arranged on the side of the inner box, and a screening box is arranged on the lower side of the inner box. The integrated peanut drying and seed coat peeling device to prevent the blockage of the air suction port is equipped with a screening box, and the screening box performs secondary screening on peanuts and red seed coats through vibration, so that the red coat testa can be removed. more thorough.

The invention discloses a breeding method for importing false smut resistance of indica rice into japonica rice. The method includes the following steps: (1) selecting japonica rice varieties / series to be improved and false smut resistant resources of the indica rice; (2) identifying the crude toxin tolerance concentration of the selected japonica rice varieties / series to be improved; (3) cross-breeding to obtain three-way cross coeno-species F1; (4) culturing anther of the three-way cross coeno-species F1 in a culture medium for culturing rows of indica rice; (5) field-planting flower cultured seedlings: planting a single anther cultured seedling to obtain a plurality of japonica rice flower cultured families with the false smut resistance improved, and further planting different flower cultured families in the field and screening to obtain new japonica rice varieties with good agronomic shape and improved false smut resistance, using the japonica rice varieties / series to be improved as a contrast. The breeding method provided by the invention can effectively import the false smut resistance of indica rice into japonica rice, and the false smut resistance breeding can be finished in about three years, therefore, the normal false smut resistance breeding period is greatly shortened; the breeding method has a great breeding application value.

The invention relates to a mutant that improves the thermal stability of heparanase I based on molecular dynamics analysis of enzyme flexibility, and the mutant is mutant BtHepI Q198R and / or mutant BtHepI K247W , the mutant BtHepI Q198R The amino acid sequence is SEQ ID NO.1, the mutant BtHepI K247W The amino acid sequence of is SEQ ID NO.2. The mutant of the present invention is a heparanase I mutant strain with excellent thermal stability, which has great potential for industrial application and economic value. Operational stability and batch reuse in catalytic processes, reducing production costs, etc., are of great importance.