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Method for breeding autotetraploid saussurea involucrata plant

A technology of autotetraploid and cultivation method, which is applied in the field of cultivation of rare ethnic medicine Tianshan Saussurea autotetraploid plants, to achieve the effects of strong pertinence, high efficiency, and accurate and reliable results

Inactive Publication Date: 2011-01-19
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can quickly realize the cultivation of autotetraploid Saussurea tianshanensis plants, thereby effectively increasing the content of its active ingredients, and overcoming the problem of species degradation in natural reproduction and artificial planting of Saussurea tianshanensis

Method used

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  • Method for breeding autotetraploid saussurea involucrata plant
  • Method for breeding autotetraploid saussurea involucrata plant
  • Method for breeding autotetraploid saussurea involucrata plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Soak the Tianshan snow lotus seeds with warm water for 20min, then disinfect the surface with 75% ethanol for 20sec, and then use 15% H 2 o 2 The solution was sterilized for 15 minutes, and the sterilized seeds were rinsed 3 times with sterile water, and then sowed on MS medium, and 10 seeds were sown in each bottle of MS medium, and the seeds of snow lotus seeds could germinate to produce sterile seedlings after 7-15 days; The germination rate was 73.3% (number of normal germinated seeds / total number of tested seeds).

[0033] Excise the roots and leaves of the germinated sterile seedlings, and keep the hypocotyl stem section;

[0034] The hypocotyl stem segment was inoculated into the germination induction medium MS+BA0.3mg / L+TDZ0.3mg / L+NAA0.05mg / L added with 2% dimethyl sulfoxide and treated for 3d (control), culture temperature 25-30℃, light intensity 50μmol m -2 ·s -1 , light time 16h·d -1 ;

[0035] Transfer the treated hypocotyl stem segment to MS+BA0.5mg / L...

Embodiment 2

[0043] Soak the Tianshan snow lotus seeds with warm water for 20min, then disinfect the surface with 75% ethanol for 20sec, and then use 15% H 2 o 2 The solution was sterilized for 15 minutes, and the sterilized seeds were rinsed twice with sterile water, and then sowed on MS medium, and 15 seeds were sown in each bottle of MS medium, and the seeds of snow lotus seeds could germinate to produce sterile seedlings after 7-15 days; The germination rate was 73.3% (number of normal germinated seeds / total number of tested seeds).

[0044] Excise the roots and leaves of the germinated sterile seedlings, and keep the hypocotyl stem section;

[0045] Inoculate the hypocotyl stem section into the germination induction medium MS+BA0.3mg / L+TDZ0.3mg / L+NAA0.05mg / L added with 2% DMSO and 0.05% colchicine for treatment 3d, culture temperature 25℃, light intensity 50μmol·m -2 ·s -1 , light time 16h·d -1 ;

[0046] Transfer the treated hypocotyl stem segment to MS+BA0.5mg / L+TDZ0.5mg / L+NAA...

Embodiment 3

[0054] Soak the Tianshan snow lotus seeds in warm water for 20min, then disinfect the surface with 75% ethanol for 30sec, and then wash them with 15% H 2 o 2 The solution was sterilized for 30 minutes, and the sterilized seeds were rinsed 4 times with sterile water, and then sowed on the MS medium, and 20 seeds were sown in each bottle of MS medium, and the seeds of snow lotus seeds could germinate to produce sterile seedlings after 7-15 days; The germination rate was 75% (number of normal germinated seeds / total number of seeds tested).

[0055] Excise the roots and leaves of the germinated sterile seedlings, and keep the hypocotyl stem section;

[0056] Inoculate the hypocotyl stem segment into the germination induction medium MS+BA0.4mg / L+TDZ0.4mg / L+NAA0.08mg / L supplemented with 2% DMSO and 0.10% colchicine for 2 days , the culture temperature was 28°C, and the light intensity was 50 μmol m -2 ·s -1 , light time 16h·d -1 ;

[0057] Transfer the treated hypocotyl stem s...

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Abstract

The invention relates to a method for breeding a saussurea involucrata plant. In the method, the lower hypocotyl stem section of the saussureae involucratae aseptic seedling after cutting off root and leaves is used as the explant, the explant is induced by using solid culture media with different concentrations of colchicine to generate a tetraploid saussureae involucratae plant, and the tetraploid saussureae involucratae is identified by using the tetraploid screening identification technology. The method provides an improvement method for optimal colchicine concentration, processing time and processing temperature for inducing generation of tetraploid, and morphology, chromosome counting and flow cytometry for identifying tetraploid. The method can be used for quickly breeding the autotetraploid saussureae involucratae plants; and the method shortens the breeding cycle, thereby effectively increasing the content of active ingredients, and overcomes the defect of variety degeneration of the saussureae involucratae in natural propagation and artificial planting.

Description

technical field [0001] The invention relates to the field of medicinal plant breeding, in particular to a method for cultivating autotetraploid plants of the rare ethnic medicine Tianshan Saussurea chinensis. Background technique [0002] Tianshan Snow Lotus (Saussurea involucrata Kar et Kir.) is a large perennial herbaceous plant with one-time flowering and fruiting in the Asteraceae family, also known as Saussurea involucrata Kar et Kir. It is a national third-class endangered species and a species endemic to Xinjiang. It grows on sub-alpine and alpine cliffs and in ice-stained rock crevices at an altitude of 2400-4100m. It is a rare and precious Chinese herbal medicine and alpine flower unique to Xinjiang, which has important medicinal and ornamental value. Snow lotus is resistant to low temperature and wind and cold, has extremely harsh growth environment, low natural reproduction rate (in the wild environment, the seed germination rate is less than 5%), long growth cyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 康喜亮王晓军郝秀英刘敏波拉提徐琴
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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